Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Anabaena sp. |
expression in Escherichia coli | Nostoc sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00176 | - |
(phosphate)45 | pH 7.5, 28°C | Nostoc sp. | |
0.00176 | - |
(Phosphate)n | at pH 7.5 and 28°C | Anabaena sp. | |
0.118 | - |
D-glucose | pH 7.5, 28°C | Nostoc sp. | |
0.118 | - |
D-glucose | at pH 7.5 and 28°C | Anabaena sp. | |
24.3 | - |
D-mannose | K0.5 value, pH 7.5, 28°C | Nostoc sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme is strictly dependent on the presence of divalent cations | Nostoc sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26000 | - |
- |
Nostoc sp. |
26000 | - |
x * 26000, SDS-PAGE | Anabaena sp. |
26600 | - |
- |
Nostoc sp. |
26600 | - |
- |
Anabaena sp. |
39000 | - |
gel filtration | Nostoc sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(phosphate)n + D-glucose | Anabaena sp. | - |
(phosphate)n-1 + D-glucose 6-phosphate | - |
? | |
(phosphate)n + D-glucose | Anabaena sp. PCC 7120 | - |
(phosphate)n-1 + D-glucose 6-phosphate | - |
? | |
(phosphate)n + mannose | Anabaena sp. | - |
(phosphate)n-1 + mannose 6-phosphate | - |
? | |
(phosphate)n + mannose | Anabaena sp. PCC 7120 | - |
(phosphate)n-1 + mannose 6-phosphate | - |
? | |
additional information | Anabaena sp. | no activity with ATP, GTP, UTP, CTP, ADP, AMP, and diphosphate | ? | - |
? | |
additional information | Anabaena sp. PCC 7120 | no activity with ATP, GTP, UTP, CTP, ADP, AMP, and diphosphate | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Anabaena sp. | Q8YX46 | - |
- |
Anabaena sp. PCC 7120 | Q8YX46 | - |
- |
Nostoc sp. | Q8YX46 | - |
- |
Nostoc sp. PCC 7120 | Q8YX46 | - |
- |
Purification (Comment) | Organism |
---|---|
glutathione Sepharose column chromatography, and gel filtration | Anabaena sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(phosphate)45 + D-glucose | - |
Nostoc sp. | (phosphate)44 + D-glucose 6-phosphate | - |
? | |
(phosphate)45 + D-glucose | - |
Nostoc sp. PCC 7120 | (phosphate)44 + D-glucose 6-phosphate | - |
? | |
(phosphate)45 + D-mannose | - |
Nostoc sp. | (phosphate)44 + D-mannose 6-phosphate | - |
? | |
(phosphate)45 + D-mannose | - |
Nostoc sp. PCC 7120 | (phosphate)44 + D-mannose 6-phosphate | - |
? | |
(phosphate)n + D-glucose | - |
Anabaena sp. | (phosphate)n-1 + D-glucose 6-phosphate | - |
? | |
(phosphate)n + D-glucose | - |
Anabaena sp. PCC 7120 | (phosphate)n-1 + D-glucose 6-phosphate | - |
? | |
(phosphate)n + mannose | - |
Anabaena sp. | (phosphate)n-1 + mannose 6-phosphate | - |
? | |
(phosphate)n + mannose | - |
Anabaena sp. PCC 7120 | (phosphate)n-1 + mannose 6-phosphate | - |
? | |
additional information | no activity with ATP, GTP, UTP, CTP, ADP, AMP, and diphosphate | Anabaena sp. | ? | - |
? | |
additional information | enzyme requires polyphosphate to confer its enzymic activity. The reaction follows a bi-bi ping-pong mechanism. No substrates: ATP, GTP, UTP, CTP, ADP, AMP, diphosphate | Nostoc sp. | ? | - |
? | |
additional information | no activity with ATP, GTP, UTP, CTP, ADP, AMP, and diphosphate | Anabaena sp. PCC 7120 | ? | - |
? | |
additional information | enzyme requires polyphosphate to confer its enzymic activity. The reaction follows a bi-bi ping-pong mechanism. No substrates: ATP, GTP, UTP, CTP, ADP, AMP, diphosphate | Nostoc sp. PCC 7120 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 26000, SDS-PAGE | Anabaena sp. |
? | x * 26600, calculated from amino acid sequence | Anabaena sp. |
dimer | or monomer, 2 * 26000, SDS-PAGE, 2 * 26600, calculated | Nostoc sp. |
monomer | or dimer, 1 * 26000, SDS-PAGE, 1 * 26600, calculated | Nostoc sp. |
Synonyms | Comment | Organism |
---|---|---|
All1371 | - |
Anabaena sp. |
All1371 | - |
Nostoc sp. |
polyphosphate glucokinase | - |
Anabaena sp. |
polyphosphate-dependent glucokinase | - |
Anabaena sp. |
PPGK | - |
Anabaena sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.21 | - |
D-mannose | pH 7.5, 28°C | Nostoc sp. | |
0.21 | - |
mannose | at pH 7.5 and 28°C | Anabaena sp. | |
48.2 | - |
(phosphate)45 | pH 7.5, 28°C | Nostoc sp. | |
48.2 | - |
D-glucose | at pH 7.5 and 28°C | Anabaena sp. | |
48.2 | - |
(Phosphate)n | at pH 7.5 and 28°C | Anabaena sp. |
General Information | Comment | Organism |
---|---|---|
physiological function | a mutant strain lacking enzymatic activity is impaired under nitrogen-fixing conditions | Nostoc sp. |