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Literature summary for 2.7.1.63 extracted from

  • Koide, M.; Miyanaga, A.; Kudo, F.; Eguchi, T.
    Characterization of polyphosphate glucokinase SCO5059 from Streptomyces coelicolor A3(2) (2013), Biosci. Biotechnol. Biochem., 77, 2322-2324.
    View publication on PubMed
Search for more literature for 2.7.1.63

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Streptomyces coelicolor
expression in Escherichia coli Streptomyces coelicolor

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
 (phosphate)6 pH 7.7, 28°C Streptomyces coelicolor
0.004
-
 (Phosphate)n at pH 7.7 and 28°C Streptomyces coelicolor
0.012
-
 D-glucose pH 7.7, 28°C Streptomyces coelicolor
0.012
-
 D-glucose at pH 7.7 and 28°C Streptomyces coelicolor

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
-
 Streptomyces coelicolor
200000
-
 gel filtration Streptomyces coelicolor

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(phosphate)n + D-glucose Streptomyces coelicolor
-
 (phosphate)n-1 + D-glucose 6-phosphate
-
 ?
(phosphate)n + D-glucose Streptomyces coelicolor A3(2)
-
 (phosphate)n-1 + D-glucose 6-phosphate
-
 ?
(phosphate)n + D-glucose Streptomyces coelicolor ATCC BAA-471
-
 (phosphate)n-1 + D-glucose 6-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces coelicolor Q9ADE8
-
-
Streptomyces coelicolor A3(2) Q9ADE8
-
-
Streptomyces coelicolor ATCC BAA-471 Q9ADE8
-
-

Purification (Commentary)

Purification (Comment) Organism
Talon metal affinity column chromatography and PD-10 gel filtration Streptomyces coelicolor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(phosphate)6 + D-glucose
-
 Streptomyces coelicolor (phosphate)5 + D-glucose 6-phosphate
-
 ?
(phosphate)6 + D-glucose
-
 Streptomyces coelicolor A3(2) (phosphate)5 + D-glucose 6-phosphate
-
 ?
(phosphate)6 + D-glucose
-
 Streptomyces coelicolor ATCC BAA-471 (phosphate)5 + D-glucose 6-phosphate
-
 ?
(phosphate)n + D-glucose
-
 Streptomyces coelicolor (phosphate)n-1 + D-glucose 6-phosphate
-
 ?
(phosphate)n + D-glucose
-
 Streptomyces coelicolor A3(2) (phosphate)n-1 + D-glucose 6-phosphate
-
 ?
(phosphate)n + D-glucose
-
 Streptomyces coelicolor ATCC BAA-471 (phosphate)n-1 + D-glucose 6-phosphate
-
 ?
ATP + D-glucose
-
 Streptomyces coelicolor ADP + D-glucose 6-phosphate allosteric behavior in presence of ATP, no activity below 1.5 mM ATP ?
ATP + D-glucose
-
 Streptomyces coelicolor A3(2) ADP + D-glucose 6-phosphate allosteric behavior in presence of ATP, no activity below 1.5 mM ATP ?
ATP + D-glucose
-
 Streptomyces coelicolor ATCC BAA-471 ADP + D-glucose 6-phosphate allosteric behavior in presence of ATP, no activity below 1.5 mM ATP ?

Subunits

Subunits Comment Organism
oligomer x * 28000, calculated, His-tagged protein Streptomyces coelicolor

Synonyms

Synonyms Comment Organism
polyphosphate glucokinase
-
 Streptomyces coelicolor
PP-Glk
-
 Streptomyces coelicolor
SCO5059
-
 Streptomyces coelicolor

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
-
 Streptomyces coelicolor

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.25
-
 D-glucose pH 7.7, 28°C Streptomyces coelicolor
0.3
-
 D-glucose at pH 7.7 and 28°C Streptomyces coelicolor
0.3
-
 (Phosphate)n at pH 7.7 and 28°C Streptomyces coelicolor
0.31
-
 (phosphate)6 pH 7.7, 28°C Streptomyces coelicolor

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5 10
-
 Streptomyces coelicolor