Application | Comment | Organism |
---|---|---|
molecular biology | GNE-deficient cells, with dramatically increased incorporation of N-acetylmannosamine analogues into glycoproteins, can efficiently be decorated with reactive functional groups, which can be employed in bioorthogonal functionalization strategies for fluorescence labelling or biotinylation | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression of GFP-tagged enzyme in HEK-293 cells, the expression is efficiently blocked by sh70-shRNA, sh68 has no effect on GNE protein expression because its target sequence at the 3'-UTR is not present in the GFP-hGNE vector construct | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | stable knock-down of UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase in HEK-293 cells by RNAi dramatically increases incorporation of N-acetylmannosamine analogues into glycoproteins of HEK-293 cells. HEK293 cells transfected with sh68- and sh70-shRNA almost completely loose GNE mRNA expression, while other shRNAs are less effective. Peanut agglutinin, recognizing asialo structures, reveals a 3-4fold higher binding affinity to GNE knock-down cells compared to controls | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Synonyms | Comment | Organism |
---|---|---|
GNE | - |
Homo sapiens |
UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
malfunction | stable knock-down of UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase in HEK-293 cells dramatically increases incorporation of N-acetylmannosamine analogues into glycoproteins of HEK-293 cells | Homo sapiens |
metabolism | UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase is the key enzyme in the sialic acid biosynthetic pathway | Homo sapiens |