Literature summary for 2.7.1.60 extracted from

Moeller, H.; Boehrsch, V.; Lucka, L.; Hackenberger, C.P.; Hinderlich, S.
Efficient metabolic oligosaccharide engineering of glycoproteins by UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase (GNE) knock-down (2011), Mol. Biosyst., 7, 2245-2251.

Search for more literature for 2.7.1.60

Application

Application	Comment	Organism
molecular biology	GNE-deficient cells, with dramatically increased incorporation of N-acetylmannosamine analogues into glycoproteins, can efficiently be decorated with reactive functional groups, which can be employed in bioorthogonal functionalization strategies for fluorescence labelling or biotinylation	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
expression of GFP-tagged enzyme in HEK-293 cells, the expression is efficiently blocked by sh70-shRNA, sh68 has no effect on GNE protein expression because its target sequence at the 3'-UTR is not present in the GFP-hGNE vector construct	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
additional information	stable knock-down of UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase in HEK-293 cells by RNAi dramatically increases incorporation of N-acetylmannosamine analogues into glycoproteins of HEK-293 cells. HEK293 cells transfected with sh68- and sh70-shRNA almost completely loose GNE mRNA expression, while other shRNAs are less effective. Peanut agglutinin, recognizing asialo structures, reveals a 3-4fold higher binding affinity to GNE knock-down cells compared to controls	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Synonyms

Synonyms	Comment	Organism
GNE	-	Homo sapiens
UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase	-	Homo sapiens

General Information

General Information	Comment	Organism
malfunction	stable knock-down of UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase in HEK-293 cells dramatically increases incorporation of N-acetylmannosamine analogues into glycoproteins of HEK-293 cells	Homo sapiens
metabolism	UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase is the key enzyme in the sialic acid biosynthetic pathway	Homo sapiens

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)