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Literature summary for 2.7.1.60 extracted from

  • Blume, A.; Ghaderi, D.; Liebich, V.; Hinderlich, S.; Donner, P.; Reutter, W.; Lucka, L.
    UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase, functionally expressed in and purified from Escherichia coli, yeast, and insect cells (2004), Protein Expr. Purif., 35, 387-396.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed with or without His-tag in Escherichia coli BL21 Star(DE3), Saccharomyces cerevisiae, Pichia pastoris, High five, Sf9, and Sf-900 cells, low yield in Escherichia coli and yeast, high expression of enzyme in High five, and Sf-900 cells Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
79000
-
6 * 79000 Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + N-acyl-D-mannosamine Rattus norvegicus key enzyme in the synthesis of N-acetylneuraminic acid and therefore of nearly all other sialic acids ADP + N-acyl-D-mannosamine 6-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzymes from all species, purification from Sf-900 can yield milligram amounts of soluble, active protein Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10.9
-
purified enzyme from Sf-900 cells, pH 8.1, 37°C Rattus norvegicus

Storage Stability

Storage Stability Organism
4°C, enzyme can be stored without substantial loss of activity Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + N-acyl-D-mannosamine
-
Rattus norvegicus ADP + N-acyl-D-mannosamine 6-phosphate
-
?
ATP + N-acyl-D-mannosamine key enzyme in the synthesis of N-acetylneuraminic acid and therefore of nearly all other sialic acids Rattus norvegicus ADP + N-acyl-D-mannosamine 6-phosphate
-
?

Subunits

Subunits Comment Organism
hexamer 6 * 79000 Rattus norvegicus

Synonyms

Synonyms Comment Organism
UDP-GlcNAc 2-epimerase/ManNAc kinase
-
Rattus norvegicus
UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase two different enzyme activities combined in a single bifunctional enzyme Rattus norvegicus

pH Stability

pH Stability pH Stability Maximum Comment Organism
1 2 pH above 12 results in almost complete disaggregation and complete loss of activity Rattus norvegicus
6
-
pH below 6 results in almost complete disaggregation and complete loss of activity Rattus norvegicus