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Literature summary for 2.7.1.60 extracted from

  • Blume, A.; Weidemann, W.; Stelzl, U.; Wanker, E.E.; Lucka, L.; Donner, P.; Reutter, W.; Horstkorte, R.; Hinderlich, S.
    Domain-specific characteristics of the bifunctional key enzyme of sialic acid biosynthesis, UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase (2004), Biochem. J., 384, 599-607.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed as His-tag fusion protein in Sf9, and Sf-900 cells Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
DELTA1-234 monomer molecular mass of 58000 Da, complete loss of epimerase activity, 90% loss of kinase activity Rattus norvegicus
DELTA1-359 monomer molecular mass of 45000 Da, complete loss of epimerase activity, almost complete loss of kinase activity Rattus norvegicus
DELTA1-39 monomer molecular mass of 79000 Da, complete loss of epimerase activity, 75% loss of kinase activity Rattus norvegicus
DELTA383-722 monomer molecular mass of 49000 Da, almost complete loss of epimerase activity, complete loss of kinase activity Rattus norvegicus
DELTA490-722 monomer molecular mass of 61000 Da, more than 80% loss of epimerase activity, complete loss of kinase activity Rattus norvegicus
DELTA597-722 monomer molecular mass of 72000 Da, almost complete loss of epimerase activity, and kinase activity Rattus norvegicus
DELTA697-722 monomer molecular mass of 83000 Da, 25% loss of epimerase activity, 60% loss of kinase activity Rattus norvegicus
DELTA717-722 monomer molecular mass of 85000 Da, less than 10% loss of epimerase activity, 20% loss of kinase activity Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
79000
-
6 * 79000, His-tagged protein has a mass of 85000 Da, SDS-PAGE, native mass by gel filtration Rattus norvegicus
500000
-
gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + N-acyl-D-mannosamine Rattus norvegicus key enzyme in the synthesis of N-acetylneuraminic acid and therefore of nearly all other sialic acids ADP + N-acyl-D-mannosamine 6-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + N-acyl-D-mannosamine
-
Rattus norvegicus ADP + N-acyl-D-mannosamine 6-phosphate
-
?
ATP + N-acyl-D-mannosamine key enzyme in the synthesis of N-acetylneuraminic acid and therefore of nearly all other sialic acids Rattus norvegicus ADP + N-acyl-D-mannosamine 6-phosphate
-
?

Subunits

Subunits Comment Organism
hexamer 6 * 79000, His-tagged protein has a mass of 85000 Da, SDS-PAGE, native mass by gel filtration Rattus norvegicus
trimer some of the deletion mutants tends to form trimers Rattus norvegicus

Synonyms

Synonyms Comment Organism
UDP-GlcNAc 2-epimerase/ManNAc kinase
-
Rattus norvegicus
UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase two different enzyme activities combined in a single bifunctional enzyme, N-terminal region is for epimerase activity, C-terminal region is for kinase activity Rattus norvegicus