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Literature summary for 2.7.1.6 extracted from

  • McAuley, M.; Mesa-Torres, N.; McFall, A.; Morris, S.; Huang, M.; Pey, A.L.; Timson, D.J.
    Improving the activity and stability of human galactokinase for therapeutic and biotechnological applications (2018), ChemBioChem, 19, 1088-1095 .
    View publication on PubMed
Search for more literature for 2.7.1.6

Application

Application Comment Organism
medicine the enzyme has biotechnological potential in enzyme replacement therapy for type II galactosaemia Homo sapiens

Protein Variants

Protein Variants Comment Organism
M185L statistically significantly increased catalytic turnover, with corresponding increases in the Michaelis constants for both substrates ATP and alpha-D-galactose. The specificity constants for galactose and ATP are not significantly changed. This variant is significantly less thermally stable than the wild-type protein and is less resistant to denaturation by urea Homo sapiens
M60V/M180V modest, but significant, increase in the melting temperature compared to the wild type, but no change in the turnover number Homo sapiens
M60V/M180V/A334S none of the steady state kinetic parameters is significantly altered. The variant has significantly increased thermal stability Homo sapiens
M60V/M180V/A334S/D268E/G373S increase in thermal stability, but no significant change in the kinetic parameters Homo sapiens
M60V/M180V/A334S/G373S increase in thermal stability, but no significant change in the kinetic parameters Homo sapiens
M60V/M180V/D268E/A334S/R366Q/G373S mutant enzyme with increased thermal stability and increased turnover towards some substrates. The conformation of the protein is altered at key sites. The number of salt bridges and hydrogen bonds is increased. More stable towards denaturation by urea Homo sapiens
Y379W a variant with greater substrate promiscuity than wild-type enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0027
-
 ATP pH 7.5, 37°C, mutant enzyme M185L/Y379W, cosubstrate: D-galactosamine Homo sapiens
0.0037
-
 ATP pH 7.5, 37°C, mutant enzyme Y379W, cosubstrate: D-galactosamine Homo sapiens
0.004
-
 ATP pH 7.5, 37°C, mutant enzyme M60V/M185L/M180V/D268E/A334S/R366Q/G373S/Y379W, cosubstrate: 2-deoxy-D-galactose Homo sapiens
0.0044
-
 ATP pH 7.5, 37°C, mutant enzyme M60V/M185L/M180V/D268E/A334S/R366Q/G373S/Y379W, cosubstrate: D-galactosamine Homo sapiens
0.0066
-
 ATP pH 7.5, 37°C, mutant enzyme M60V/M180V/D268E/A334S/R366Q/G373S/Y379W, cosubstrate: D-galactosamine Homo sapiens
0.0134
-
 ATP pH 7.5, 37°C, mutant enzyme M60V/M180V/D268E/A334S/R366Q/G373S/Y379W, cosubstrate: D-talose Homo sapiens
0.016
-
 ATP pH 7.5, 37°C, mutant enzyme Y379W, cosubstrate: 2-deoxy-D-galactose Homo sapiens
0.0173
-
 ATP pH 7.5, 37°C, mutant enzyme M185L, cosubstrate: 2-deoxy-D-galactose Homo sapiens
0.0174
-
 ATP pH 7.5, 37°C, mutant enzyme M60V/M180V/D268E/A334S/R366Q/G373S/Y379W, cosubstrate: 2-deoxy-D-galactose Homo sapiens
0.0432
-
 ATP pH 7.5, 37°C, wild-type enzyme, cosubstrate: 2-deoxy-D-galactose Homo sapiens
0.078
-
 alpha-D-galactose pH 7.5, 37°C, mutant enzyme M60V/M180V/A334S Homo sapiens
0.09
-
 alpha-D-galactose pH 7.5, 37°C, mutant enzyme G373S Homo sapiens
0.099
-
 alpha-D-galactose pH 7.5, 37°C, mutant enzyme M180V Homo sapiens
0.15
-
 alpha-D-galactose pH 7.5, 37°C, mutant enzyme M60V/M180V/A334S/D268E/R336Q/G373S Homo sapiens
0.162
-
 ATP pH 7.5, 37°C, wild-type enzyme, cosubstrate: D-galactosamine Homo sapiens
0.17
-
 alpha-D-galactose pH 7.5, 37°C, wild type enzyme Homo sapiens
0.17
-
 ATP pH 7.5, 37°C, wild-type enzyme, cosubstrate: D-talose Homo sapiens
0.19
-
 alpha-D-galactose pH 7.5, 37°C, mutant enzyme M60V/M180V Homo sapiens
0.22
-
 alpha-D-galactose pH 7.5, 37°C, mutant enzyme M60V Homo sapiens
0.22
-
 D-galactosamine pH 7.5, 37°C, mutant enzyme Y379W Homo sapiens
0.23
-
 alpha-D-galactose pH 7.5, 37°C, mutant enzyme M60V/M180V/A334S/G373S Homo sapiens
0.27
-
 D-galactosamine pH 7.5, 37°C, mutant enzyme M185L/Y379W Homo sapiens
0.27
-
 alpha-D-galactose pH 7.5, 37°C, mutant enzyme M60V/M180V/A334S/D268E/G373S Homo sapiens
0.28
-
 alpha-D-galactose pH 7.5, 37°C, mutant enzyme A334S Homo sapiens
0.3
-
 2-deoxy-D-galactose pH 7.5, 37°C, mutant enzyme M60V/M185L/M180V/D268E/A334S/R366Q/G373S/Y379W Homo sapiens
0.31
-
 alpha-D-galactose pH 7.5, 37°C, mutant enzyme M60V/M180V/D268E/A334S/R366Q/G373S/Y379W Homo sapiens
0.37
-
 D-talose pH 7.5, 37°C, wild-type enzyme Homo sapiens
0.48
-
 alpha-D-galactose pH 7.5, 37°C, mutant enzyme M60V/A334S Homo sapiens
0.53
-
 alpha-D-galactose pH 7.5, 37°C, mutant enzyme R366Q Homo sapiens
0.57
-
 ATP pH 7.5, 37°C, mutant enzyme M60V/M180V/A334S/G373S, cosubstrate: alpha-D-galactose Homo sapiens
0.58
-
 alpha-D-galactose pH 7.5, 37°C, mutant enzyme D268E Homo sapiens
0.62
-
 D-galactosamine pH 7.5, 37°C, mutant enzyme M60V/M185L/M180V/D268E/A334S/R366Q/G373S/Y379W Homo sapiens
0.71
-
 D-galactosamine pH 7.5, 37°C, wild-type enzyme Homo sapiens
0.82
-
 ATP pH 7.5, 37°C, mutant enzyme M60V/M180V/A334S, cosubstrate: alpha-D-galactose Homo sapiens
0.97
-
 2-deoxy-D-galactose pH 7.5, 37°C, wild-type enzyme Homo sapiens
1
-
 ATP pH 7.5, 37°C, mutant enzyme M60V/M180V, cosubstrate: alpha-D-galactose Homo sapiens
1.12
-
 alpha-D-galactose pH 7.5, 37°C, mutant enzyme M185L/Y379W Homo sapiens
1.19
-
 alpha-D-galactose pH 7.5, 37°C, mutant enzyme M185L Homo sapiens
1.32
-
 alpha-D-galactose pH 7.5, 37°C, mutant enzyme Y379W Homo sapiens
2
-
 2-deoxy-D-galactose pH 7.5, 37°C, mutant enzyme M60V/M180V/D268E/A334S/R366Q/G373S/Y379W Homo sapiens
2.2
-
 D-talose pH 7.5, 37°C, mutant enzyme M60V/M180V/D268E/A334S/R366Q/G373S/Y379W Homo sapiens
2.2
-
 2-deoxy-D-galactose pH 7.5, 37°C, mutant enzyme Y379W Homo sapiens
2.5
-
 ATP pH 7.5, 37°C, mutant enzyme M60V/M180V/A334S/D268E/G373S, cosubstrate: alpha-D-galactose Homo sapiens
3.278
-
 alpha-D-galactose pH 7.5, 37°C, mutant enzyme M60V/M185L/M180V/D268E/A334S/R366Q/G373S/Y379W Homo sapiens
3.8
-
 ATP pH 7.5, 37°C, mutant enzyme M60V/M180V/A334S/D268E/R336Q/G373S, cosubstrate: alpha-D-galactose Homo sapiens
3.9
-
 ATP pH 7.5, 37°C, wild type enzyme, cosubstrate: alpha-D-galactose Homo sapiens
4.2
-
 ATP pH 7.5, 37°C, mutant enzyme M60V/A334S, cosubstrate: alpha-D-galactose Homo sapiens
4.8
-
 D-galactosamine pH 7.5, 37°C, mutant enzyme M60V/M180V/D268E/A334S/R366Q/G373S/Y379W Homo sapiens
5
-
 ATP pH 7.5, 37°C, mutant enzyme Y379W, cosubstrate: alpha-D-galactose Homo sapiens
5.4
-
 ATP pH 7.5, 37°C, mutant enzyme M180V, cosubstrate: alpha-D-galactose Homo sapiens
7.4
-
 2-deoxy-D-galactose pH 7.5, 37°C, mutant enzyme M185L Homo sapiens
7.7
-
 ATP pH 7.5, 37°C, mutant enzyme R366Q, cosubstrate: alpha-D-galactose Homo sapiens
7.9
-
 ATP pH 7.5, 37°C, mutant enzyme A334S, cosubstrate: alpha-D-galactose Homo sapiens
8.1
-
 ATP pH 7.5, 37°C, mutant enzyme M60V/M180V/D268E/A334S/R366Q/G373S/Y379W, cosubstrate: alpha-D-galactose Homo sapiens
11.4
-
 ATP pH 7.5, 37°C, mutant enzyme M185L/Y379W, cosubstrate: alpha-D-galactose Homo sapiens
12.4
-
 ATP pH 7.5, 37°C, mutant enzyme M60V/M185L/M180V/D268E/A334S/R366Q/G373S/Y379W, cosubstrate: alpha-D-galactose Homo sapiens
13
-
 ATP pH 7.5, 37°C, mutant enzyme D268E, cosubstrate: alpha-D-galactose Homo sapiens
13
-
 ATP pH 7.5, 37°C, mutant enzyme G373S, cosubstrate: alpha-D-galactose Homo sapiens
18
-
 ATP pH 7.5, 37°C, mutant enzyme M60V, cosubstrate: alpha-D-galactose Homo sapiens
34.5
-
 ATP pH 7.5, 37°C, mutant enzyme M185L, cosubstrate: alpha-D-galactose Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + alpha-D-galactose Homo sapiens
-
 ADP + alpha-D-galactose 1-phosphate
-
 ?

Organic Solvent Stability

Organic Solvent Comment Organism
urea 37°C, 30 min, the mutant enzyme M60V/M180V/D268E/A334S/R366Q/G373S is more stable towards urea than the wild-type enzyme Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P51570
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2-deoxy-D-galactose
-
 Homo sapiens ADP + 2-deoxy-D-galactose 1-phosphate
-
 ?
ATP + alpha-D-galactose
-
 Homo sapiens ADP + alpha-D-galactose 1-phosphate
-
 ?
ATP + D-galactosamine
-
 Homo sapiens ADP + alpha-D-galactosamine 1-phosphate
-
 ?
ATP + D-talose
-
 Homo sapiens ADP + alpha-D-talose 1-phosphate
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50.33
-
 Tm-value, mutant enzyme M185L/Y379W Homo sapiens
50.51
-
 Tm-value, mutant enzyme M185L Homo sapiens
52.07
-
 Tm-value, mutant enzyme Y379W Homo sapiens
52.31
-
 Tm-value, mutant enzyme A334S/M60V Homo sapiens
52.31
-
 Tm-value, mutant enzyme D268E Homo sapiens
53.83
-
 Tm-value, mutant enzyme M60V/M185L/M180V/D268E/A334S/R366Q/G373S/Y379W Homo sapiens
54.41
-
 Tm-value, mutant enzyme A334S Homo sapiens
55.01
-
 Tm-value, mutant enzyme G373S Homo sapiens
55.17
-
 Tm-value, mutant enzyme M180V Homo sapiens
55.74
-
 Tm-value, wild type enzyme Homo sapiens
55.81
-
 Tm-value, mutant enzyme R366Q Homo sapiens
55.93
-
 Tm-value, mutant enzyme M60V Homo sapiens
57.14
-
 Tm-value, mutant enzyme M60V/M180V/D268E/A334S/R366Q/G373S/Y379W Homo sapiens
57.7
-
 Tm-value, mutant enzyme M60V/M180V Homo sapiens
58.08
-
 Tm-value, mutant enzyme M180V/M60V/A334S Homo sapiens
58.09
-
 Tm-value, mutant enzyme M180V/M60V/A334S/G373S/D268E Homo sapiens
58.11
-
 Tm-value, mutant enzyme M180V/M60V/A334S/G373S Homo sapiens
58.12
-
 Tm-value, mutant enzyme M60V/M180V/A334S/D268E/R336Q/G373S Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.2
-
 D-talose pH 7.5, 37°C, mutant enzyme M60V/M180V/D268E/A334S/R366Q/G373S/Y379W Homo sapiens
0.2
-
 ATP pH 7.5, 37°C, mutant enzyme M60V/M180V/D268E/A334S/R366Q/G373S/Y379W, cosubstrate: D-talose Homo sapiens
0.2
-
 D-galactosamine pH 7.5, 37°C, mutant enzyme Y379W Homo sapiens
0.2
-
 ATP pH 7.5, 37°C, mutant enzyme Y379W, cosubstrate: D-galactosamine Homo sapiens
0.3
-
 D-galactosamine pH 7.5, 37°C, mutant enzyme M60V/M185L/M180V/D268E/A334S/R366Q/G373S/Y379W Homo sapiens
0.3
-
 ATP pH 7.5, 37°C, mutant enzyme M60V/M185L/M180V/D268E/A334S/R366Q/G373S/Y379W, cosubstrate: D-galactosamine Homo sapiens
0.4
-
 2-deoxy-D-galactose pH 7.5, 37°C, mutant enzyme M185L Homo sapiens
0.7
-
 2-deoxy-D-galactose pH 7.5, 37°C, mutant enzyme M60V/M185L/M180V/D268E/A334S/R366Q/G373S/Y379W Homo sapiens
0.7
-
 ATP pH 7.5, 37°C, mutant enzyme M60V/M185L/M180V/D268E/A334S/R366Q/G373S/Y379W, cosubstrate: 2-deoxy-D-galactose Homo sapiens
0.8
-
 2-deoxy-D-galactose pH 7.5, 37°C, mutant enzyme Y379W Homo sapiens
0.8
-
 ATP pH 7.5, 37°C, mutant enzyme Y379W, cosubstrate: 2-deoxy-D-galactose Homo sapiens
1
-
 alpha-D-galactose pH 7.5, 37°C, mutant enzyme M180V Homo sapiens
1
-
 ATP pH 7.5, 37°C, mutant enzyme M180V, cosubstrate: alpha-D-galactose Homo sapiens
1.5
-
 alpha-D-galactose pH 7.5, 37°C, mutant enzyme M60V/M180V Homo sapiens
1.5
-
 ATP pH 7.5, 37°C, mutant enzyme M60V/M180V, cosubstrate: alpha-D-galactose Homo sapiens
1.6
-
 alpha-D-galactose pH 7.5, 37°C, mutant enzyme M60V/M180V/A334S/D268E/R336Q/G373S Homo sapiens
1.6
-
 ATP pH 7.5, 37°C, mutant enzyme M60V/M180V/A334S/D268E/R336Q/G373S, cosubstrate: alpha-D-galactose Homo sapiens
1.7
-
 D-galactosamine pH 7.5, 37°C, wild-type enzyme Homo sapiens
1.7
-
 alpha-D-galactose pH 7.5, 37°C, mutant enzyme M60V/M180V/A334S Homo sapiens
1.7
-
 ATP pH 7.5, 37°C, mutant enzyme M60V/M180V/A334S, cosubstrate: alpha-D-galactose Homo sapiens
1.7
-
 alpha-D-galactose pH 7.5, 37°C, mutant enzyme M60V/M180V/A334S/D268E/G373S Homo sapiens
1.7
-
 ATP pH 7.5, 37°C, mutant enzyme M60V/M180V/A334S/D268E/G373S, cosubstrate: alpha-D-galactose Homo sapiens
1.7
-
 alpha-D-galactose pH 7.5, 37°C, mutant enzyme M60V/M180V/A334S/G373S Homo sapiens
1.7
-
 ATP pH 7.5, 37°C, mutant enzyme M60V/M180V/A334S/G373S, cosubstrate: alpha-D-galactose Homo sapiens
1.7
-
 alpha-D-galactose pH 7.5, 37°C, wild type enzyme Homo sapiens
1.7
-
 ATP pH 7.5, 37°C, wild type enzyme, cosubstrate: alpha-D-galactose Homo sapiens
1.7
-
 ATP pH 7.5, 37°C, wild-type enzyme, cosubstrate: D-galactosamine Homo sapiens
2
-
 D-talose pH 7.5, 37°C, wild-type enzyme Homo sapiens
2
-
 ATP pH 7.5, 37°C, wild-type enzyme, cosubstrate: D-talose Homo sapiens
2.1
-
 alpha-D-galactose pH 7.5, 37°C, mutant enzyme R366Q Homo sapiens
2.1
-
 ATP pH 7.5, 37°C, mutant enzyme R366Q, cosubstrate: alpha-D-galactose Homo sapiens
2.4
-
 alpha-D-galactose pH 7.5, 37°C, mutant enzyme G373S Homo sapiens
2.4
-
 ATP pH 7.5, 37°C, mutant enzyme G373S, cosubstrate: alpha-D-galactose Homo sapiens
2.5
-
 alpha-D-galactose pH 7.5, 37°C, mutant enzyme M185L/Y379W Homo sapiens
2.5
-
 ATP pH 7.5, 37°C, mutant enzyme M185L/Y379W, cosubstrate: alpha-D-galactose Homo sapiens
2.6
-
 alpha-D-galactose pH 7.5, 37°C, mutant enzyme M60V/A334S Homo sapiens
2.6
-
 ATP pH 7.5, 37°C, mutant enzyme M60V/A334S, cosubstrate: alpha-D-galactose Homo sapiens
3.2
-
 alpha-D-galactose pH 7.5, 37°C, mutant enzyme D268E Homo sapiens
3.2
-
 ATP pH 7.5, 37°C, mutant enzyme D268E, cosubstrate: alpha-D-galactose Homo sapiens
4
-
 D-galactosamine pH 7.5, 37°C, mutant enzyme M60V/M180V/D268E/A334S/R366Q/G373S/Y379W Homo sapiens
4
-
 ATP pH 7.5, 37°C, mutant enzyme M60V/M180V/D268E/A334S/R366Q/G373S/Y379W, cosubstrate: D-galactosamine Homo sapiens
4.3
-
 alpha-D-galactose pH 7.5, 37°C, mutant enzyme A334S Homo sapiens
4.3
-
 ATP pH 7.5, 37°C, mutant enzyme A334S, cosubstrate: alpha-D-galactose Homo sapiens
4.8
-
 alpha-D-galactose pH 7.5, 37°C, mutant enzyme M60V Homo sapiens
4.8
-
 ATP pH 7.5, 37°C, mutant enzyme M60V, cosubstrate: alpha-D-galactose Homo sapiens
5
-
 alpha-D-galactose pH 7.5, 37°C, mutant enzyme M60V/M180V/D268E/A334S/R366Q/G373S/Y379W Homo sapiens
5
-
 ATP pH 7.5, 37°C, mutant enzyme M60V/M180V/D268E/A334S/R366Q/G373S/Y379W, cosubstrate: alpha-D-galactose Homo sapiens
6.8
-
 2-deoxy-D-galactose pH 7.5, 37°C, wild-type enzyme Homo sapiens
6.8
-
 2-deoxy-D-galactose pH 7.5, 37°C, mutant enzyme M60V/M180V/D268E/A334S/R366Q/G373S/Y379W Homo sapiens
6.8
-
 ATP pH 7.5, 37°C, mutant enzyme M60V/M180V/D268E/A334S/R366Q/G373S/Y379W, cosubstrate: 2-deoxy-D-galactose Homo sapiens
6.8
-
 alpha-D-galactose pH 7.5, 37°C, mutant enzyme Y379W Homo sapiens
6.8
-
 ATP pH 7.5, 37°C, mutant enzyme Y379W, cosubstrate: alpha-D-galactose Homo sapiens
6.8
-
 ATP pH 7.5, 37°C, wild-type enzyme, cosubstrate: 2-deoxy-D-galactose Homo sapiens
8.1
-
 alpha-D-galactose pH 7.5, 37°C, mutant enzyme M185L Homo sapiens
8.1
-
 ATP pH 7.5, 37°C, mutant enzyme M185L, cosubstrate: alpha-D-galactose Homo sapiens
9.4
-
 ATP pH 7.5, 37°C, mutant enzyme M185L, cosubstrate: 2-deoxy-D-galactose Homo sapiens
12.9
-
 alpha-D-galactose pH 7.5, 37°C, mutant enzyme M60V/M185L/M180V/D268E/A334S/R366Q/G373S/Y379W Homo sapiens
12.9
-
 ATP pH 7.5, 37°C, mutant enzyme M60V/M185L/M180V/D268E/A334S/R366Q/G373S/Y379W, cosubstrate: alpha-D-galactose Homo sapiens
25
-
 D-galactosamine pH 7.5, 37°C, mutant enzyme M185L/Y379W Homo sapiens
25
-
 ATP pH 7.5, 37°C, mutant enzyme M185L/Y379W, cosubstrate: D-galactosamine Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.006
-
 2-deoxy-D-galactose pH 7.5, 37°C, mutant enzyme M185L Homo sapiens
0.2
-
 D-talose pH 7.5, 37°C, mutant enzyme M60V/M180V/D268E/A334S/R366Q/G373S/Y379W Homo sapiens
0.3
-
 D-galactosamine pH 7.5, 37°C, mutant enzyme Y379W Homo sapiens
0.39
-
 2-deoxy-D-galactose pH 7.5, 37°C, mutant enzyme Y379W Homo sapiens
0.4
-
 D-galactosamine pH 7.5, 37°C, mutant enzyme M60V/M185L/M180V/D268E/A334S/R366Q/G373S/Y379W Homo sapiens
0.9
-
 D-galactosamine pH 7.5, 37°C, mutant enzyme M60V/M180V/D268E/A334S/R366Q/G373S/Y379W Homo sapiens
2.3
-
 2-deoxy-D-galactose pH 7.5, 37°C, mutant enzyme M60V/M185L/M180V/D268E/A334S/R366Q/G373S/Y379W Homo sapiens
2.4
-
 alpha-D-galactose pH 7.5, 37°C, mutant enzyme M185L/Y379W Homo sapiens
2.6
-
 D-talose pH 7.5, 37°C, wild-type enzyme Homo sapiens
2.9
-
 D-galactosamine pH 7.5, 37°C, wild-type enzyme Homo sapiens
3.1
-
 2-deoxy-D-galactose pH 7.5, 37°C, mutant enzyme M60V/M180V/D268E/A334S/R366Q/G373S/Y379W Homo sapiens
3.9
-
 alpha-D-galactose pH 7.5, 37°C, mutant enzyme M60V/M185L/M180V/D268E/A334S/R366Q/G373S/Y379W Homo sapiens
4
-
 alpha-D-galactose pH 7.5, 37°C, mutant enzyme R366Q Homo sapiens
5.2
-
 alpha-D-galactose pH 7.5, 37°C, mutant enzyme Y379W Homo sapiens
5.4
-
 alpha-D-galactose pH 7.5, 37°C, mutant enzyme M60V/A334S Homo sapiens
6
-
 alpha-D-galactose pH 7.5, 37°C, mutant enzyme D268E Homo sapiens
6.3
-
 alpha-D-galactose pH 7.5, 37°C, mutant enzyme M60V/M180V/A334S/D268E/G373S Homo sapiens
7.1
-
 2-deoxy-D-galactose pH 7.5, 37°C, wild-type enzyme Homo sapiens
7.3
-
 alpha-D-galactose pH 7.5, 37°C, mutant enzyme M60V/M180V/A334S/G373S Homo sapiens
8
-
 alpha-D-galactose pH 7.5, 37°C, mutant enzyme M185L Homo sapiens
8.8
-
 alpha-D-galactose pH 7.5, 37°C, mutant enzyme M60V/M180V Homo sapiens
10.1
-
 alpha-D-galactose pH 7.5, 37°C, mutant enzyme M180V Homo sapiens
10.1
-
 alpha-D-galactose pH 7.5, 37°C, wild type enzyme Homo sapiens
10.9
-
 alpha-D-galactose pH 7.5, 37°C, mutant enzyme M60V/M180V/A334S/D268E/R336Q/G373S Homo sapiens
15.4
-
 alpha-D-galactose pH 7.5, 37°C, mutant enzyme A334S Homo sapiens
16.1
-
 alpha-D-galactose pH 7.5, 37°C, mutant enzyme M60V/M180V/D268E/A334S/R366Q/G373S/Y379W Homo sapiens
17.6
-
 ATP pH 7.5, 37°C, mutant enzyme M60V/M180V/D268E/A334S/R366Q/G373S/Y379W, cosubstrate: D-talose Homo sapiens
19.4
-
 ATP pH 7.5, 37°C, mutant enzyme M60V/M185L/M180V/D268E/A334S/R366Q/G373S/Y379W, cosubstrate: 2-deoxy-D-galactose Homo sapiens
20
-
 alpha-D-galactose pH 7.5, 37°C, mutant enzyme M60V/M180V/A334S Homo sapiens
21.8
-
 alpha-D-galactose pH 7.5, 37°C, mutant enzyme M60V Homo sapiens
23
-
 ATP pH 7.5, 37°C, mutant enzyme M185L, cosubstrate: 2-deoxy-D-galactose Homo sapiens
25
-
 alpha-D-galactose pH 7.5, 37°C, mutant enzyme G373S Homo sapiens
50
-
 ATP pH 7.5, 37°C, mutant enzyme Y379W, cosubstrate: 2-deoxy-D-galactose Homo sapiens
50
-
 ATP pH 7.5, 37°C, mutant enzyme Y379W, cosubstrate: D-galactosamine Homo sapiens
67
-
 ATP pH 7.5, 37°C, mutant enzyme M60V/M185L/M180V/D268E/A334S/R366Q/G373S/Y379W, cosubstrate: D-galactosamine Homo sapiens
91
-
 D-galactosamine pH 7.5, 37°C, mutant enzyme M185L/Y379W Homo sapiens
110
-
 ATP pH 7.5, 37°C, wild-type enzyme, cosubstrate: D-galactosamine Homo sapiens
110
-
 ATP pH 7.5, 37°C, wild-type enzyme, cosubstrate: D-talose Homo sapiens
185
-
 ATP pH 7.5, 37°C, mutant enzyme M180V, cosubstrate: alpha-D-galactose Homo sapiens
190
-
 ATP pH 7.5, 37°C, mutant enzyme G373S, cosubstrate: alpha-D-galactose Homo sapiens
190
-
 ATP pH 7.5, 37°C, wild-type enzyme, cosubstrate: 2-deoxy-D-galactose Homo sapiens
220
-
 ATP pH 7.5, 37°C, mutant enzyme M185L/Y379W, cosubstrate: alpha-D-galactose Homo sapiens
236
-
 ATP pH 7.5, 37°C, mutant enzyme M185L, cosubstrate: alpha-D-galactose Homo sapiens
240
-
 ATP pH 7.5, 37°C, mutant enzyme D268E, cosubstrate: alpha-D-galactose Homo sapiens
270
-
 ATP pH 7.5, 37°C, mutant enzyme M60V, cosubstrate: alpha-D-galactose Homo sapiens
270
-
 ATP pH 7.5, 37°C, mutant enzyme R366Q, cosubstrate: alpha-D-galactose Homo sapiens
390
-
 ATP pH 7.5, 37°C, mutant enzyme M60V/M180V/A334S/D268E/R336Q/G373S, cosubstrate: alpha-D-galactose Homo sapiens
390
-
 ATP pH 7.5, 37°C, mutant enzyme M60V/M180V/D268E/A334S/R366Q/G373S/Y379W, cosubstrate: 2-deoxy-D-galactose Homo sapiens
430
-
 ATP pH 7.5, 37°C, wild type enzyme, cosubstrate: alpha-D-galactose Homo sapiens
550
-
 ATP pH 7.5, 37°C, mutant enzyme A334S, cosubstrate: alpha-D-galactose Homo sapiens
550
-
 ATP pH 7.5, 37°C, mutant enzyme M60V/M180V/D268E/A334S/R366Q/G373S/Y379W, cosubstrate: alpha-D-galactose Homo sapiens
600
-
 ATP pH 7.5, 37°C, mutant enzyme M60V/M180V/D268E/A334S/R366Q/G373S/Y379W, cosubstrate: D-galactosamine Homo sapiens
620
-
 ATP pH 7.5, 37°C, mutant enzyme M60V/A334S, cosubstrate: alpha-D-galactose Homo sapiens
680
-
 ATP pH 7.5, 37°C, mutant enzyme M60V/M180V/A334S/D268E/G373S, cosubstrate: alpha-D-galactose Homo sapiens
950
-
 ATP pH 7.5, 37°C, mutant enzyme M185L/Y379W, cosubstrate: D-galactosamine Homo sapiens
1000
-
 ATP pH 7.5, 37°C, mutant enzyme M60V/M185L/M180V/D268E/A334S/R366Q/G373S/Y379W Homo sapiens
1360
-
 ATP pH 7.5, 37°C, mutant enzyme Y379W, cosubstrate: alpha-D-galactose Homo sapiens
1430
-
 ATP pH 7.5, 37°C, mutant enzyme M60V/M180V, cosubstrate: alpha-D-galactose Homo sapiens
2000
-
 ATP pH 7.5, 37°C, mutant enzyme M60V/M180V/A334S, cosubstrate: alpha-D-galactose Homo sapiens
3000
-
 ATP pH 7.5, 37°C, mutant enzyme M60V/M180V/A334S/G373S, cosubstrate: alpha-D-galactose Homo sapiens