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Literature summary for 2.7.1.6 extracted from
- The characterisation of a galactokinase from Streptomyces coelicolor (2019), Carbohydr. Res., 472, 132-137 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli | Streptomyces coelicolor |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 5 mM | Streptomyces coelicolor |  |
| Cu2+ | 5 mM | Streptomyces coelicolor | |
| EDTA | 5 mM | Streptomyces coelicolor | |
| Mn2+ | 5 mM | Streptomyces coelicolor | |
| Ni2+ | 5 mM | Streptomyces coelicolor | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.035 | - |
ATP | pH 7.4, 37°C | Streptomyces coelicolor | |
| 0.627 | - |
alpha-D-galactose | pH 7.4, 37°C | Streptomyces coelicolor | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 5 mM, required | Streptomyces coelicolor | |
| Zn2+ | 5 mM, slight activation | Streptomyces coelicolor | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + alpha-D-galactose | Streptomyces coelicolor | involved in Leloir pathway | ADP + alpha-D-galactose 1-phosphate | - |
? | |
| ATP + alpha-D-galactose | Streptomyces coelicolor A3(2) | involved in Leloir pathway | ADP + alpha-D-galactose 1-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces coelicolor | Q9K3S8 | - |
- |
| Streptomyces coelicolor A3(2) | Q9K3S8 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Streptomyces coelicolor |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 3-deoxy-D-galactose | 85% conversion after 2 h under optimised conditions | Streptomyces coelicolor | ADP + 3-deoxy-D-galactose 1-phosphate | - |
? | |
| ATP + 3-deoxy-D-galactose | 85% conversion after 2 h under optimised conditions | Streptomyces coelicolor A3(2) | ADP + 3-deoxy-D-galactose 1-phosphate | - |
? | |
| ATP + alpha-D-galactose | involved in Leloir pathway | Streptomyces coelicolor | ADP + alpha-D-galactose 1-phosphate | - |
? | |
| ATP + alpha-D-galactose | 91% conversion after 2 h under optimised conditions | Streptomyces coelicolor | ADP + alpha-D-galactose 1-phosphate | - |
? | |
| ATP + alpha-D-galactose | involved in Leloir pathway | Streptomyces coelicolor A3(2) | ADP + alpha-D-galactose 1-phosphate | - |
? | |
| ATP + alpha-D-galactose | 91% conversion after 2 h under optimised conditions | Streptomyces coelicolor A3(2) | ADP + alpha-D-galactose 1-phosphate | - |
? | |
| ATP + D-galactosamine | 87% conversion after 2 h under optimised conditions | Streptomyces coelicolor | ADP + D-galactosamine 1-phosphate | - |
? | |
| ATP + D-galactosamine | 87% conversion after 2 h under optimised conditions | Streptomyces coelicolor A3(2) | ADP + D-galactosamine 1-phosphate | - |
? | |
| ATP + L-arabinose | 10% conversion after 2 h under optimised conditions | Streptomyces coelicolor | ADP + L-arabinose 1-phosphate | - |
? | |
| ATP + N-acetyl-D-galactosamine | 40% conversion after 2 h under optimised conditions | Streptomyces coelicolor | ADP + N-acetyl-alpha-D-galactosamine 1-phosphate | - |
? | |
| ATP + N-acetyl-D-galactosamine | 40% conversion after 2 h under optimised conditions | Streptomyces coelicolor A3(2) | ADP + N-acetyl-alpha-D-galactosamine 1-phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GALK | - |
Streptomyces coelicolor |
| SCO3136 | - |
Streptomyces coelicolor |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Streptomyces coelicolor |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 5 | 50 | 5°C: about 60% of maximal activity, 55°C: about 50% of maximal activity | Streptomyces coelicolor |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.35 | - |
ATP | pH 7.4, 37°C | Streptomyces coelicolor | |
| 1.35 | - |
alpha-D-galactose | pH 7.4, 37°C | Streptomyces coelicolor | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 10 | - |
Streptomyces coelicolor |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 10 | pH 4.0: about 90% of maximal activity, pH 10.0: about 90% of maximal activity | Streptomyces coelicolor |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.15 | - |
alpha-D-galactose | pH 7.4, 37°C | Streptomyces coelicolor | |
| 38.9 | - |
ATP | pH 7.4, 37°C | Streptomyces coelicolor | |
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Release 2023.1 (January 2023)
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