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Literature summary for 2.7.1.6 extracted from

  • Stambolian, D.; Scarpino-Myers, V.; Harris, H.
    Purification of human galactokinase and evidence for its existence as a monomer form (1985), Biochim. Biophys. Acta, 831, 306-312.
    View publication on PubMed

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000 38000 gel filtration Homo sapiens
38000
-
1 * 38000, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte
-
Homo sapiens
-
placenta
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.62
-
enzyme from erythrocyte Homo sapiens
1.737
-
enzyme from placenta Homo sapiens

Storage Stability

Storage Stability Organism
-20°C, unstable in 0.01 M KH2PO4-K2HPO4, pH 7.2 buffer, 0.01 M MgCl2, 1 mM dithiothreitol, 0.2 mM ATP without glycerol Homo sapiens
4°C, 0.01 M KH2PO4-K2HPO4, pH 7.2 buffer, 0.01 M MgCl2, 1 mM dithiothreitol, about 40% loss of activity after 24 h Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-galactose
-
Homo sapiens ADP + alpha-D-galactose 1-phosphate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 38000, SDS-PAGE Homo sapiens

pI Value

Organism Comment pI Value Maximum pI Value
Homo sapiens isoelectric focusing
-
5.7