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Literature summary for 2.7.1.59 extracted from
- Purification and some properties of inducible N-acetylglucosamine kinase from Candida albicans (1980), Biochim. Biophys. Acta, 614, 350-356.
General Stability
| General Stability | Organism |
|---|---|
| stable to dialysis | Candida albicans |
| unstable to freezing and thawing, heat and low pH | Candida albicans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| N-ethylmaleimide | - |
Candida albicans |  |
| p-chloromercuribenzoate | - |
Candida albicans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.33 | - |
N-acetyl-D-glucosamine | 30°C, pH 7.6 | Candida albicans | |
| 1.82 | - |
ATP | 30°C, pH 7.6 | Candida albicans | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | can partially replace Mg2+ in activation | Candida albicans | |
| Ca2+ | can partially replace Mg2+ in activation | Candida albicans | |
| Co2+ | can partially replace Mg2+ in activation | Candida albicans | |
| Mg2+ | required | Candida albicans | |
| Mn2+ | can partially replace Mg2+ in activation | Candida albicans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 75000 | - |
gel filtration | Candida albicans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + N-acetyl-D-glucosamine | Candida albicans | first reaction of inducible N-acetylglucosamine catabolic pathway, enables organism to grow on N-acetylglucosamine as sole carbon source | ADP + N-acetyl-D-glucosamine 6-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Candida albicans | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Candida albicans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 9.5 | - |
purified enzyme, 30°C, pH 7.6 | Candida albicans |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0-2°C, 20 mM potassium phosphate, pH 7.6, 1 mM EDTA, 10 mM 2-mercaptoethanol, stable for at least 1 week | Candida albicans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + N-acetyl-D-glucosamine | highly specific | Candida albicans | ADP + N-acetyl-D-glucosamine 6-phosphate | - |
? | |
| ATP + N-acetyl-D-glucosamine | first reaction of inducible N-acetylglucosamine catabolic pathway, enables organism to grow on N-acetylglucosamine as sole carbon source | Candida albicans | ADP + N-acetyl-D-glucosamine 6-phosphate | - |
? | |
| CTP + N-acetyl-D-glucosamine | - |
Candida albicans | CDP + N-acetyl-D-glucosamine 6-phosphate | - |
? | |
| dATP + N-acetyl-D-glucosamine | - |
Candida albicans | dADP + N-acetyl-D-glucosamine 6-phosphate | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
complete inactivation with 2 min | Candida albicans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | - |
Candida albicans |
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