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Literature summary for 2.7.1.59 extracted from

  • Asensio, C.; Ruiz-Amil, M.
    N-Acetyl-D-glucosamine kinase. II. Escherichia coli (1966), Methods Enzymol., 9, 421-425.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
ADP strong competitive inhibitor Escherichia coli
D-glucosamine
-
Escherichia coli
N-Methyl-D-glucosamine
-
Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00005
-
N-acetyl-D-glucosamine 37°C, pH 7.8 Escherichia coli
0.00014
-
ATP 37°C, pH 7.8 Escherichia coli
0.00025
-
ITP 37°C, pH 7.8 Escherichia coli
0.005
-
D-glucose 37°C, pH 7.8 Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + N-acetyl-D-glucosamine Escherichia coli
-
ADP + N-acetyl-D-glucosamine 6-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.6
-
purified enzyme, 37°C, pH 7.8 Escherichia coli

Storage Stability

Storage Stability Organism
-20°C, stable for several months Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-glucose 15% of the rate with N-acetyl-D-glucosamine Escherichia coli ADP + D-glucose 6-phosphate
-
?
ATP + N-acetyl-D-glucosamine
-
Escherichia coli ADP + N-acetyl-D-glucosamine 6-phosphate
-
?
ITP + N-acetyl-D-glucosamine 75% of the activity with ATP Escherichia coli IDP + N-acetyl-D-glucosamine 6-phosphate
-
?
additional information N-methyl-D-glucosamine, D-glucosamine, N-acetyl-D-galactosamine, N-acetyl-D-mannosamine do not serve as substrates, very low activities with UTP, GTP and CTP Escherichia coli ?
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
heat treatment for 5 min included in purification procedure Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 9.5
-
Escherichia coli

pH Stability

pH Stability pH Stability Maximum Comment Organism
9.5
-
no loss of activity within 30 min at 30°C Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.001
-
N-Methyl-D-glucosamine 37°C, pH 7.8 Escherichia coli
0.01
-
D-glucosamine 37°C, pH 7.8 Escherichia coli