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show all sequences of 2.7.1.58

Structure of 2-oxo-3-deoxygalactonate kinase from Klebsiella pneumoniae

Michalska, K.; Cuff, M.E.; Tesar, C.; Feldmann, B.; Joachimiak, A.; Acta Crystallogr. Sect. D 67, 678-689 (2011)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli strain BL21 (DE3) from plasmid pRK1037 harboring a fusion protein construct containing an N-terminal maltose-binding protein followed by a tobacco vein mottling virus protease cleavage site, a His6 -tag, a TEV protease cleavage site and the target protein 2-oxo-3-deoxygalactonate kinase
Klebsiella pneumoniae
Crystallization (Commentary)
Crystallization
Organism
purified recombinant wild-type and mselenomethionine-labeled enzymes, sitting-drop vapor-diffusion, mixing of 0.0004 ml of 30 mg/m protein in 20 mM HEPES, pH 8.0, 250 mM NaCl, and 2 mM dithiothreitol, with 0.0004 ml of crystallization solution containing 0.1 M magnesium formate, 15%(w/v) PEG 3350, equilibration against 0.135 ml of crystallization solution, at 4°C or 24°C, method optimization, X-ray diffraction structure determination and analysis at 2.1 A resolution
Klebsiella pneumoniae
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Klebsiella pneumoniae
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
31100
-
el filtration, recombinant detagged enzyme
Klebsiella pneumoniae
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + 2-dehydro-3-deoxy-D-galactonate
Klebsiella pneumoniae
-
ADP + 2-dehydro-3-deoxy-6-phospho-D-galactonate
-
-
?
ATP + 2-dehydro-3-deoxy-D-galactonate
Klebsiella pneumoniae MGH 78578
-
ADP + 2-dehydro-3-deoxy-6-phospho-D-galactonate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Klebsiella pneumoniae
A6TFZ6
subsp. pneumoniae
-
Klebsiella pneumoniae MGH 78578
A6TFZ6
subsp. pneumoniae
-
Purification (Commentary)
Commentary
Organism
recombinant N-terminally maltose-binding protein fused , His6 -tagged enzyme from Escherichia coli strain BL21 (DE3) by amylose and nickel affinity chromatographies interveined by the cleavage of the tags, to homogeneity, followed by ultrafiltration
Klebsiella pneumoniae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + 2-dehydro-3-deoxy-D-galactonate
-
721158
Klebsiella pneumoniae
ADP + 2-dehydro-3-deoxy-6-phospho-D-galactonate
-
-
-
?
ATP + 2-dehydro-3-deoxy-D-galactonate
-
721158
Klebsiella pneumoniae MGH 78578
ADP + 2-dehydro-3-deoxy-6-phospho-D-galactonate
-
-
-
?
Subunits
Subunits
Commentary
Organism
More
the enzyme is composed of two alpha/betaa domains
Klebsiella pneumoniae
Cofactor
Cofactor
Commentary
Organism
Structure
ATP
-
Klebsiella pneumoniae
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli strain BL21 (DE3) from plasmid pRK1037 harboring a fusion protein construct containing an N-terminal maltose-binding protein followed by a tobacco vein mottling virus protease cleavage site, a His6 -tag, a TEV protease cleavage site and the target protein 2-oxo-3-deoxygalactonate kinase
Klebsiella pneumoniae
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
ATP
-
Klebsiella pneumoniae
Crystallization (Commentary) (protein specific)
Crystallization
Organism
purified recombinant wild-type and mselenomethionine-labeled enzymes, sitting-drop vapor-diffusion, mixing of 0.0004 ml of 30 mg/m protein in 20 mM HEPES, pH 8.0, 250 mM NaCl, and 2 mM dithiothreitol, with 0.0004 ml of crystallization solution containing 0.1 M magnesium formate, 15%(w/v) PEG 3350, equilibration against 0.135 ml of crystallization solution, at 4°C or 24°C, method optimization, X-ray diffraction structure determination and analysis at 2.1 A resolution
Klebsiella pneumoniae
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Klebsiella pneumoniae
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
31100
-
el filtration, recombinant detagged enzyme
Klebsiella pneumoniae
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + 2-dehydro-3-deoxy-D-galactonate
Klebsiella pneumoniae
-
ADP + 2-dehydro-3-deoxy-6-phospho-D-galactonate
-
-
?
ATP + 2-dehydro-3-deoxy-D-galactonate
Klebsiella pneumoniae MGH 78578
-
ADP + 2-dehydro-3-deoxy-6-phospho-D-galactonate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant N-terminally maltose-binding protein fused , His6 -tagged enzyme from Escherichia coli strain BL21 (DE3) by amylose and nickel affinity chromatographies interveined by the cleavage of the tags, to homogeneity, followed by ultrafiltration
Klebsiella pneumoniae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + 2-dehydro-3-deoxy-D-galactonate
-
721158
Klebsiella pneumoniae
ADP + 2-dehydro-3-deoxy-6-phospho-D-galactonate
-
-
-
?
ATP + 2-dehydro-3-deoxy-D-galactonate
-
721158
Klebsiella pneumoniae MGH 78578
ADP + 2-dehydro-3-deoxy-6-phospho-D-galactonate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
More
the enzyme is composed of two alpha/betaa domains
Klebsiella pneumoniae
General Information
General Information
Commentary
Organism
evolution
the enzyme belongs to the ASKHA (acetate and sugar kinases/hsc70/actin) family of phosphotransferases, ASKHA signature motifs in KDGal kinase, overview
Klebsiella pneumoniae
metabolism
the 2-oxo-3-deoxygalactonate kinase catalyzes the penultimate step of an alternative route of D-galactose assimilation, the so-called De Ley-Doudoroff pathway, in which D-galactose is metabolized into pyruvate and D-glyceraldehyde 3-phosphate in five consecutive reactions carried out by specific enzymes. The pathway involves the phosphorylation of 2-oxo-3-deoxygalactonate to 2-oxo-3-deoxygalactonate 6-phosphate catalyzed by the enzyme with ATP serving as a phosphoryl-group donor. Comparison with other D-galactose metabolic pathways, overview. 2-oxo-3-deoxygalactonate 6-phosphate decomposes into puruvate and D-glyceraldehyde 3-phosphate, both products are utilized by the glycolytic pathway
Klebsiella pneumoniae
additional information
the putative substrate-binding site is created mostly by the N-terminal domain, substrate binding includes the conserved Tyr78 from loop L6, His141 and Lys143 from L15, as well as Glu119 from the H4 helix, nucleotide-binding site structure, overview
Klebsiella pneumoniae
General Information (protein specific)
General Information
Commentary
Organism
evolution
the enzyme belongs to the ASKHA (acetate and sugar kinases/hsc70/actin) family of phosphotransferases, ASKHA signature motifs in KDGal kinase, overview
Klebsiella pneumoniae
metabolism
the 2-oxo-3-deoxygalactonate kinase catalyzes the penultimate step of an alternative route of D-galactose assimilation, the so-called De Ley-Doudoroff pathway, in which D-galactose is metabolized into pyruvate and D-glyceraldehyde 3-phosphate in five consecutive reactions carried out by specific enzymes. The pathway involves the phosphorylation of 2-oxo-3-deoxygalactonate to 2-oxo-3-deoxygalactonate 6-phosphate catalyzed by the enzyme with ATP serving as a phosphoryl-group donor. Comparison with other D-galactose metabolic pathways, overview. 2-oxo-3-deoxygalactonate 6-phosphate decomposes into puruvate and D-glyceraldehyde 3-phosphate, both products are utilized by the glycolytic pathway
Klebsiella pneumoniae
additional information
the putative substrate-binding site is created mostly by the N-terminal domain, substrate binding includes the conserved Tyr78 from loop L6, His141 and Lys143 from L15, as well as Glu119 from the H4 helix, nucleotide-binding site structure, overview
Klebsiella pneumoniae
Other publictions for EC 2.7.1.58
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738267
Pickl
Identification and characteriz ...
Haloferax volcanii
FEMS Microbiol. Lett.
361
76-83
2014
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1
1
721158
Michalska
Structure of 2-oxo-3-deoxygala ...
Klebsiella pneumoniae, Klebsiella pneumoniae MGH 78578
Acta Crystallogr. Sect. D
67
678-689
2011
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3
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641690
Brechtel
L-Glucitol catabolism in Steno ...
Stenotrophomonas maltophilia
Appl. Environ. Microbiol.
68
582-587
2002
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1
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1
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1
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1
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5090
Szumilo
Pathway for D-galactonate cata ...
Mycobacterium butyricum
J. Bacteriol.
148
368-370
1981
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1
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5088
Deacon
D-Galactonate utilisation by e ...
Escherichia coli, Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium LT2
FEBS Lett.
77
201-205
1977
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5
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43
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1
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1
12
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1
12
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641687
Stouthamer
-
Glucose and galactose metaboli ...
Gluconacetobacter liquefaciens
Biochim. Biophys. Acta
48
484-500
1961
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