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Literature summary for 2.7.1.56 extracted from
- Purification and properties of 1-phosphofructokinase from Escherichia coli (1985), FEMS Microbiol. Lett., 29, 231-235.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | kinetics | Escherichia coli |  |
| D-fructose 1,6-bisphosphate | kinetics | Escherichia coli | |
| additional information | not inhibited by D-fructose 6-phosphate; not inhibited by D-glucose 1-phosphate; not inhibited by D-glucose 6-phosphate | Escherichia coli | |
| phenylmethanesulfonyl fluoride | 1 mM, 65% irreversible inhibition, 1.5 M ATP protect | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.12 | - |
ATP | pH 7.6, 37°C | Escherichia coli | |
| 0.25 | - |
D-Fructose 1-phosphate | pH 7.6, 37°C | Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 5 mM, 25% of activity with Mg2+ | Escherichia coli | |
| Mg2+ | required for activity | Escherichia coli | |
| Mn2+ | required for activity | Escherichia coli | |
| Mn2+ | 5 mM, 50% of activity with Mg2+ | Escherichia coli | |
| additional information | not activated by Ca2+, Cu2+ and Fe2+ | Escherichia coli |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
2 * 30000, SDS-PAGE | Escherichia coli |
| 57000 | - |
gel filtration | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
K12 strain jOD5 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-cellulose, ammonium sulfate, hydroxylapatite, Sephadex G-75, DEAE-cellulose | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-fructose 1-phosphate | no activity with D-fructose 6-phosphate | Escherichia coli | ADP + D-fructose 1,6-bisphosphate | - |
? | |
| ATP + D-fructose 1-phosphate | no activity with D-glucose 6-phosphate, D-glucose 1-phosphate | Escherichia coli | ADP + D-fructose 1,6-bisphosphate | - |
? | |
| CTP + D-fructose 1-phosphate | 10% of activity with ATP | Escherichia coli | CDP + D-fructose 1,6-bisphosphate | - |
? | |
| GTP + D-fructose 1-phosphate | 60% of activity with ATP | Escherichia coli | GDP + D-fructose 1,6-bisphosphate | - |
? | |
| UTP + D-fructose 1-phosphate | 20% of activity with ATP | Escherichia coli | UDP + D-fructose 1,6-bisphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 30000, SDS-PAGE | Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
50 mM Tris-HCl | Escherichia coli |
| 8.5 | - |
50 mM NH4HCO3 | Escherichia coli |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.28 | - |
ADP | pH 7.6, 37°C | Escherichia coli | |
| 6.1 | - |
D-fructose-1,6-bisphosphate | pH 7.6, 37°C | Escherichia coli | |
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