Literature summary for 2.7.1.52 extracted from

Liu, Y.; Hu, H.; Wang, J.; Zhou, Q.; Wu, P.; Yan, N.; Wang, H.; Wu, J.; Sun, L.
Cryo-EM structure of L-fucokinase/GDP-fucose pyrophosphorylase (FKP) in Bacteroides fragilis (2019), Protein Cell, 10, 365-369 .

Protein Variants

Protein Variants	Comment	Organism
A723R	the fucokinase activity of the mutant is totally abolished	Bacteroides fragilis
D594A	the mutant retains hardly any fucokinase activity	Bacteroides fragilis
D601A	the mutant retains hardly any fucokinase activity	Bacteroides fragilis
D762A	the mutant retains hardly any fucokinase activity	Bacteroides fragilis
G597A	the mutant retains hardly any fucokinase activity	Bacteroides fragilis
G598A	the mutant retains hardly any fucokinase activity	Bacteroides fragilis
G713A	the fucokinase activity of the mutant is totally abolished	Bacteroides fragilis
G715A	the fucokinase activity of the mutant is totally abolished	Bacteroides fragilis
G717A	the fucokinase activity of the mutant is totally abolished	Bacteroides fragilis
G757A	the mutant has greatly reduced fucokinase activity compared to the wild type enzyme	Bacteroides fragilis
G758A	the mutant retains hardly any fucokinase activity	Bacteroides fragilis
G759A	the mutant retains hardly any fucokinase activity	Bacteroides fragilis
G899A	the mutant retains hardly any fucokinase activity	Bacteroides fragilis
G901A	the mutant retains hardly any fucokinase activity	Bacteroides fragilis
G902A	the mutant has greatly reduced fucokinase activity compared to the wild type enzyme	Bacteroides fragilis
Q761A	the mutant retains hardly any fucokinase activity	Bacteroides fragilis
R592A	the mutant retains hardly any fucokinase activity	Bacteroides fragilis
S714A	the fucokinase activity of the mutant is totally abolished	Bacteroides fragilis
S719A	the fucokinase activity of the mutant is totally abolished	Bacteroides fragilis
S720A	the fucokinase activity of the mutant is totally abolished	Bacteroides fragilis
T602A	the mutant has greatly reduced fucokinase activity compared to the wild type enzyme	Bacteroides fragilis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.06629	-	L-fucose	pH and temperature not specified in the publication	Bacteroides fragilis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
338000	-	ultracentrifugation sedimentation velocity analysis	Bacteroides fragilis
440000	-	gel filtration	Bacteroides fragilis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + L-fucose	Bacteroides fragilis	-	ADP + beta-L-fucose 1-phosphate	-	?
ATP + L-fucose	Bacteroides fragilis 9343	-	ADP + beta-L-fucose 1-phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Bacteroides fragilis	Q58T34	-	-
Bacteroides fragilis 9343	Q58T34	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + L-fucose	-	Bacteroides fragilis	ADP + beta-L-fucose 1-phosphate	-	?
ATP + L-fucose	-	Bacteroides fragilis 9343	ADP + beta-L-fucose 1-phosphate	-	?

Subunits

Subunits	Comment	Organism
homotetramer	4 * 105000, calculated from amino acid sequence	Bacteroides fragilis

Synonyms

Synonyms	Comment	Organism
FKP	bifunctional enzyme with L-fucokinase/GDP-fucose diphosphorylase activities	Bacteroides fragilis
L-fucokinase	-	Bacteroides fragilis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.39	-	L-fucose	pH and temperature not specified in the publication	Bacteroides fragilis