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Literature summary for 2.7.1.52 extracted from

  • Liu, T.W.; Ito, H.; Chiba, Y.; Kubota, T.; Sato, T.; Narimatsu, H.
    Functional expression of L-fucokinase/guanosine 5-diphosphate-L-fucose pyrophosphorylase from Bacteroides fragilis in Saccharomyces cerevisiae for the production of nucleotide sugars from exogenous monosaccharides (2011), Glycobiology, 21, 1228-1236.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
functional expression of HA-tagged FKP in Saccharomyces cerevisiae strain W303-1A, the biosynthesis of GDP-D-Ara in the recombinant Saccharomyces cerevisiae strain harboring the FKP gene can occur through a mechanism akin to that of GDP-L-Fuc via the salvage pathway. Expression of a loss-of-function FKP mutant in yeast Bacteroides fragilis

General Stability

General Stability Organism
instability of the enzyme occurs through chemical reduction in disulfide bonds of FKP protein, resulting in degradation Bacteroides fragilis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Bacteroides fragilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-fucose Bacteroides fragilis
-
ADP + beta-L-fucose 1-phosphate
-
?
ATP + L-fucose Bacteroides fragilis 9343
-
ADP + beta-L-fucose 1-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Bacteroides fragilis
-
-
-
Bacteroides fragilis 9343
-
-
-
no activity in Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-fucose
-
Bacteroides fragilis ADP + beta-L-fucose 1-phosphate
-
?
ATP + L-fucose
-
Bacteroides fragilis 9343 ADP + beta-L-fucose 1-phosphate
-
?
additional information fucokinase:GDP-fucose pyrophosphorylase is a dual activity enzyme metabolizing fucose and nucleotide-sugar Bacteroides fragilis ?
-
?
additional information fucokinase:GDP-fucose pyrophosphorylase is a dual activity enzyme metabolizing fucose and nucleotide-sugar Bacteroides fragilis 9343 ?
-
?

Synonyms

Synonyms Comment Organism
FKP
-
Bacteroides fragilis
Fuk
-
Bacteroides fragilis
L-fucokinase/guanosine 5'-diphosphate-L-fucose pyrophosphorylase
-
Bacteroides fragilis

Cofactor

Cofactor Comment Organism Structure
ATP
-
Bacteroides fragilis

General Information

General Information Comment Organism
metabolism the enzyme catalyzes the first step of the salvage pathway converting exogenous L-Fuc to GDP-L-Fuc Bacteroides fragilis
physiological function the bifunctional L-Fuc pyrophosphorylase, FKP, converts L-Fuc into GDP-L-Fuc via an L-Fuc-1-phosphate intermediate Bacteroides fragilis