General Stability | Organism |
---|---|
EDTA stabilizes during purification | Canis lupus familiaris |
glycerol, sucrose, DTT or 2-mercaptoethanol stabilizes | Canis lupus familiaris |
ovalbumin does not stabilize purified enzyme | Canis lupus familiaris |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-fucose | substrate inhibition at high concentrations | Canis lupus familiaris | |
L-galactose | weak | Canis lupus familiaris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.017 | - |
L-fucose | pH 6.5, 37°C | Canis lupus familiaris | |
0.026 | - |
L-fucose | pH 8.3, 37°C | Canis lupus familiaris | |
0.29 | - |
ATP | pH 6.5, pH 37°C | Canis lupus familiaris | |
0.63 | - |
ATP | pH 8.3, pH 37°C | Canis lupus familiaris |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | divalent cation required, CoSO4 exhibits 12% of the activity with MgSO4 | Canis lupus familiaris | |
Mg2+ | divalent cation required Mg2+ is slightly more effective than Mn2+ | Canis lupus familiaris | |
Mn2+ | divalent cation required, MnSO4 exhibits 90% of the activity with MgSO4 | Canis lupus familiaris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
494000 | - |
gel filtration | Canis lupus familiaris |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 6-deoxy-L-galactose | Canis lupus familiaris | i.e. L-fucose, serves to utilize free L-fucose for glycoprotein synthesis | ADP + 6-deoxy-L-galactose 1-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Canis lupus familiaris | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Canis lupus familiaris |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
thyroid gland | - |
Canis lupus familiaris | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0091 | - |
radiometric assay | Canis lupus familiaris |
Storage Stability | Organism |
---|---|
2-4°C, most stable in the presence of glycerol and a sulfhydryl reagent | Canis lupus familiaris |
frozen, in the presence of glycerol or sucrose and a sulfhydryl reagent, up to 1 week | Canis lupus familiaris |
frozen, rapid loss of activity, glycerol or sucrose and DTT or 2-mercaptoethanol stabilize, no stabilization by Mg2+, EDTA, KCl or ovalbumin | Canis lupus familiaris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 6-deoxy-L-galactose | highly specific for ATP | Canis lupus familiaris | ADP + 6-deoxy-L-galactose 1-phosphate | beta-L-fucose 1-phosphate | ? | |
ATP + 6-deoxy-L-galactose | highly specific for L-fucose | Canis lupus familiaris | ADP + 6-deoxy-L-galactose 1-phosphate | beta-L-fucose 1-phosphate | ? | |
ATP + 6-deoxy-L-galactose | i.e. L-fucose, serves to utilize free L-fucose for glycoprotein synthesis | Canis lupus familiaris | ADP + 6-deoxy-L-galactose 1-phosphate | - |
? | |
CTP + 6-deoxy-L-galactose | no activity | Canis lupus familiaris | CDP + 6-deoxy-L-galactose 1-phosphate | - |
? | |
GTP + 6-deoxy-L-galactose | no activity | Canis lupus familiaris | GDP + 6-deoxy-L-galactose 1-phosphate | - |
? | |
UTP + 6-deoxy-L-galactose | no activity | Canis lupus familiaris | UDP + 6-deoxy-L-galactose 1-phosphate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
two optima: pH 6.5 and pH 8.3 | Canis lupus familiaris |
8.3 | - |
two optima: pH 6.5 and pH 8.3 | Canis lupus familiaris |