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Literature summary for 2.7.1.50 extracted from

  • Tani, Y.; Kimura, K.; Mihara, H.
    Purification and properties of 4-methyl-5-hydroxyethylthiazole kinase from Escherichia coli (2016), Biosci. Biotechnol. Biochem., 80, 514-517.
    View publication on PubMed
Search for more literature for 2.7.1.50

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ highest activity in the presence of Co2+ Escherichia coli
Mg2+ activates Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
 x * 28000, SDS-PAGE Escherichia coli
28100
-
-
 Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P76423
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Ni-NTA column chromatography, and gel filtration Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.9
-
 with UTP as phosphate donor, in the presence of Mg2+, at pH 10.0 and 50°C Escherichia coli
1.1
-
 with CTP as phosphate donor, in the presence of Mg2+, at pH 10.0 and 50°C Escherichia coli
1.4
-
 with ATP as phosphate donor, in the presence of Mg2+, at pH 10.0 and 50°C Escherichia coli
3.2
-
 with GTP as phosphate donor, in the presence of Mg2+, at pH 10.0 and 50°C Escherichia coli
3.9
-
 with dCTP as phosphate donor, in the presence of Mg2+, at pH 10.0 and 50°C Escherichia coli
4
-
 with dUTP as phosphate donor, in the presence of Mg2+, at pH 10.0 and 50°C Escherichia coli
4.3
-
 with UTP as phosphate donor, in the presence of Co2+, at pH 10.0 and 50°C Escherichia coli
5.2
-
 with CTP as phosphate donor, in the presence of Co2+, at pH 10.0 and 50°C Escherichia coli
8.9
-
 with TTP as phosphate donor, in the presence of Mg2+, at pH 10.0 and 50°C Escherichia coli
9.2
-
 with dATP as phosphate donor, in the presence of Mg2+, at pH 10.0 and 50°C Escherichia coli
11
-
 with ATP as phosphate donor, in the presence of Co2+, at pH 10.0 and 50°C Escherichia coli
11
-
 with dGTP as phosphate donor, in the presence of Co2+, at pH 10.0 and 50°C Escherichia coli
14
-
 with dGTP as phosphate donor, in the presence of Mg2+, at pH 10.0 and 50°C Escherichia coli
14
-
 with dUTP as phosphate donor, in the presence of Co2+, at pH 10.0 and 50°C Escherichia coli
15
-
 with dCTP as phosphate donor, in the presence of Co2+, at pH 10.0 and 50°C Escherichia coli
24
-
 with GTP as phosphate donor, in the presence of Co2+, at pH 10.0 and 50°C Escherichia coli
37
-
 with dATP as phosphate donor, in the presence of Co2+, at pH 10.0 and 50°C Escherichia coli
45
-
 with TTP as phosphate donor, in the presence of Co2+, at pH 10.0 and 50°C Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 4-methyl-5-(2-hydroxyethyl)thiazole
-
 Escherichia coli ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole
-
 ?
CTP + 4-methyl-5-(2-hydroxyethyl)thiazole
-
 Escherichia coli CDP + 4-methyl-5-(2-phosphonooxyethyl)thiazole
-
 ?
dATP + 4-methyl-5-(2-hydroxyethyl)thiazole the enzyme shows a preference for dATP as a phosphate donor over ATP Escherichia coli dADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole
-
 ?
dCTP + 4-methyl-5-(2-hydroxyethyl)thiazole
-
 Escherichia coli dCDP + 4-methyl-5-(2-phosphonooxyethyl)thiazole
-
 ?
dGTP + 4-methyl-5-(2-hydroxyethyl)thiazole
-
 Escherichia coli dGDP + 4-methyl-5-(2-phosphonooxyethyl)thiazole
-
 ?
dUTP + 4-methyl-5-(2-hydroxyethyl)thiazole
-
 Escherichia coli dUDP + 4-methyl-5-(2-phosphonooxyethyl)thiazole
-
 ?
GTP + 4-methyl-5-(2-hydroxyethyl)thiazole
-
 Escherichia coli GDP + 4-methyl-5-(2-phosphonooxyethyl)thiazole
-
 ?
TTP + 4-methyl-5-(2-hydroxyethyl)thiazole
-
 Escherichia coli TDP + 4-methyl-5-(2-phosphonooxyethyl)thiazole
-
 ?
UTP + 4-methyl-5-(2-hydroxyethyl)thiazole
-
 Escherichia coli UDP + 4-methyl-5-(2-phosphonooxyethyl)thiazole
-
 ?

Subunits

Subunits Comment Organism
? x * 28000, SDS-PAGE Escherichia coli
? x * 28100, calculated from amino acid sequence Escherichia coli

Synonyms

Synonyms Comment Organism
4-methyl-5-hydroxyethylthiazole kinase
-
 Escherichia coli
ThiM
-
 Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 60 no decrease in the enzyme activity is detected at temperatures lower than 50°C, although about 60% of its original activity is lost at 60°C after 20 min Escherichia coli