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Literature summary for 2.7.1.49 extracted from

  • Park, J.H.; Burns, K.; Kinsland, C.; Begley, T.P.
    Characterization of two kinases involved in thiamine pyrophosphate and pyridoxal phosphate biosynthesis in Bacillus subtilis: 4-amino-5-hydroxymethyl-2methylpyrimidine kinase and pyridoxal kinase (2004), J. Bacteriol., 186, 1571-1573.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.49

Cloned(Commentary)

Cloned (Comment) Organism
gene annotated ThiD, instead of YjbV, subcloning in Escherichia coli strain DH5alpha, overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Bacillus subtilis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ MgCl2 at 2 mM Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 4-amino-5-hydroxymethyl-2-methylpyrimidine Bacillus subtilis
-
 ADP + 4-amino-5-phosphomethyl-2-methylpyrimidine
-
 ?
ATP + 4-amino-5-phosphomethyl-2-methylpyrimidine Bacillus subtilis enzyme also performs the reaction of EC 2.7.4.7, phosphomethylpyrimidine kinase ADP + 4-amino-5-diphosphomethyl-2-methylpyrimidine
-
 ?
additional information Bacillus subtilis the enzyme has both a biosynthetic and a salvage pathway function ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
 gene annotated ThiD, instead of YjbV
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 4-amino-5-hydroxymethyl-2-methylpyrimidine
-
 Bacillus subtilis ADP + 4-amino-5-phosphomethyl-2-methylpyrimidine
-
 ?
ATP + 4-amino-5-phosphomethyl-2-methylpyrimidine enzyme also performs the reaction of EC 2.7.4.7, phosphomethylpyrimidine kinase Bacillus subtilis ADP + 4-amino-5-diphosphomethyl-2-methylpyrimidine
-
 ?
additional information the enzyme has both a biosynthetic and a salvage pathway function Bacillus subtilis ?
-
 ?
additional information no activity with pyridoxal, pyridoxine, or pyridoxamine Bacillus subtilis ?
-
 ?

Synonyms

Synonyms Comment Organism
4-amino-5-hydroxymethyl-2-methylpyrimidine kinase
-
 Bacillus subtilis
4-amino-5-hydroxymethyl-2-methylpyrimidine pyrophosphate kinase
-
 Bacillus subtilis
ThiD
-
 Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Bacillus subtilis

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Bacillus subtilis