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Literature summary for 2.7.1.49 extracted from

  • Lewin, L.M.; Brown, G.M.
    The biosynthesis of thiamine. III. Mechanism of enzymatic formation of the pyrophosphate ester of 2-methyl-4-amino-5-hydroxymethylpyrimidine (1961), J. Biol. Chem., 236, 2768-2771.
No PubMed abstract available
Search for more literature for 2.7.1.49

General Stability

General Stability Organism
protected from inactivation by cysteine Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
additional information stable to treatment with p-hydroxymercuribenzoate Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no metal requirement Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 2-methyl-4-amino-5-hydroxymethylpyrimidine Saccharomyces cerevisiae involved in thiamine biosynthetic pathway, first steps ADP + 2-methyl-4-amino-5-phosphomethylpyrimidine
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
 bakers' yeast
-

Purification (Commentary)

Purification (Comment) Organism
partially Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2-methyl-4-amino-5-hydroxymethylpyrimidine
-
 Saccharomyces cerevisiae ADP + 2-methyl-4-amino-5-phosphomethylpyrimidine
-
 ?
ATP + 2-methyl-4-amino-5-hydroxymethylpyrimidine involved in thiamine biosynthetic pathway, first steps Saccharomyces cerevisiae ADP + 2-methyl-4-amino-5-phosphomethylpyrimidine
-
 ?
ATP + 4-amino-5-hydroxy-2-methylpyrimidine
-
 Saccharomyces cerevisiae ADP + 4-amino-2-methyl-5-phosphopyrimidine
-
 ?
CTP + 5-hydroxy-2-methylpyrimidine
-
 Saccharomyces cerevisiae CDP + 2-methyl-5-phosphopyrimidine
-
 ?
GTP + 5-hydroxy-2-methylpyrimidine
-
 Saccharomyces cerevisiae GDP + 2-methyl-5-phosphopyrimidine
-
 ?
UTP + 5-hydroxy-2-methylpyrimidine
-
 Saccharomyces cerevisiae UDP + 5-hydroxy-2-methylpyrimidine monophosphate
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 stable to heating for 10 min Saccharomyces cerevisiae