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Literature summary for 2.7.1.48 extracted from
- Purification, activity, and expression levels of two uridine-cytidine kinase isoforms in neuroblastoma cell lines (2016), Nucleosides Nucleotides Nucleic Acids, 35, 613-618 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene UCK1, recombinant expression of N-terminally His-tagged isozyme in Escherichia coli strain BL21(DE3) | Homo sapiens |
| gene UCK2, recombinant expression of N-terminally His-tagged isozyme in Escherichia coli strain BL21(DE3) | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | steady-state kinetics | Homo sapiens | |
| 0.1 | - |
cytidine | recombinant isozyme UCK2, pH 7.6, 37°C | Homo sapiens |  |
| 0.13 | - |
uridine | recombinant isozyme UCK2, pH 7.6, 37°C | Homo sapiens | |
| 4 | - |
cytidine | recombinant isozyme UCK1, pH 7.6, 37°C | Homo sapiens | |
| 5.1 | - |
uridine | recombinant isozyme UCK1, pH 7.6, 37°C | Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + cytidine | Homo sapiens | - |
ADP + CMP | - |
? | |
| ATP + uridine | Homo sapiens | - |
ADP + UMP | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q9BZX2 | - |
- |
| Homo sapiens | Q9HA47 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant N-terminally His-tagged isozyme UCK1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Homo sapiens |
| recombinant N-terminally His-tagged isozyme UCK2 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| FISK cell | - |
Homo sapiens | - |
| IMR-32 cell | - |
Homo sapiens | - |
| NASS cell | - |
Homo sapiens | - |
| neuroblastoma cell | generation of a specific antibody against UCK1 and analysis of expression of both UCK isoforms 1 and 2 in a panel of neuroblastoma cell lines, overview. No differences are observed for UCK1 and UCK2 expression between MYCN-single copy cell lines and MYCN-amplified neuroblastoma cell lines | Homo sapiens | - |
| SH-SY5Y cell | - |
Homo sapiens | - |
| SJNB-10 cell | - |
Homo sapiens | - |
| SJNB-8 cell | - |
Homo sapiens | - |
| SK-N-BE(2) cell | - |
Homo sapiens | - |
| SK-N-SH cell | - |
Homo sapiens | - |
| Tet21N cell | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + cytidine | - |
Homo sapiens | ADP + CMP | - |
? | |
| ATP + uridine | - |
Homo sapiens | ADP + UMP | - |
? | |
| additional information | uridine-cytidine kinase (UCK) catalyzes the phosphorylation of uridine, cytidine, and several pyrimidine ribonucleoside analogues | Homo sapiens | ? | - |
- |
|
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 29000, recombinant isozyme UCK2, SDS-PAGE | Homo sapiens |
| ? | x * 31000, recombinant isozyme UCK1, SDS-PAGE | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| UCK1 | - |
Homo sapiens |
| UCK2 | - |
Homo sapiens |
| uridine-cytidine kinase | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
assay at | Homo sapiens |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.59 | - |
uridine | recombinant isozyme UCK1, pH 7.6, 37°C | Homo sapiens | |
| 0.69 | - |
cytidine | recombinant isozyme UCK1, pH 7.6, 37°C | Homo sapiens | |
| 210 | - |
cytidine | recombinant isozyme UCK2, pH 7.6, 37°C | Homo sapiens | |
| 470 | - |
uridine | recombinant isozyme UCK2, pH 7.6, 37°C | Homo sapiens | |
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