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Literature summary for 2.7.1.48 extracted from

  • Cihak, A.
    Stability of the insoluble form of uridine kinase coupled to Zn2+ or Pb2+ ions (1976), Hoppe-Seyler's Z. Physiol. Chem., 357, 345-350.
    View publication on PubMed
Search for more literature for 2.7.1.48

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 1-2.5 mM, 40-50% activation of Pb2+-precipitated uridine kinase Bos taurus
dithiothreitol 1-2.5 mM, 40-50% activation of Pb2+-precipitated uridine kinase Bos taurus

General Stability

General Stability Organism
stability of insoluble metal ion precipitated form of the enzyme Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
Ag+
-
 Bos taurus
CTP 0.1 mM, complete inhibition Bos taurus

Metals/Ions

Metals/Ions Comment Organism Structure
additional information freeze dried preparations of uridine kinase precipitated by Pb2+ or Zn2+ are highly active Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
 calf
-

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Bos taurus
-

Storage Stability

Storage Stability Organism
5°C, Pb2+ or Zn2+-precipitated uridine kinase, 6 months, 10-15% loss of activity Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + uridine
-
 Bos taurus ADP + UMP
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 3% loss of activity after 10 min Bos taurus
100
-
 dry uridine kinase precipitated with Pb2+, 30% loss of activity after 1 h, dry uridine kinase precipitated with Zn2+, 25% loss of activity after 1 h Bos taurus

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 7.5 complete loss of activity below pH 4.0 Bos taurus