Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 1-2.5 mM, 40-50% activation of Pb2+-precipitated uridine kinase | Bos taurus | |
dithiothreitol | 1-2.5 mM, 40-50% activation of Pb2+-precipitated uridine kinase | Bos taurus |
General Stability | Organism |
---|---|
stability of insoluble metal ion precipitated form of the enzyme | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | - |
Bos taurus | |
CTP | 0.1 mM, complete inhibition | Bos taurus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | freeze dried preparations of uridine kinase precipitated by Pb2+ or Zn2+ are highly active | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
calf | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Bos taurus | - |
Storage Stability | Organism |
---|---|
5°C, Pb2+ or Zn2+-precipitated uridine kinase, 6 months, 10-15% loss of activity | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + uridine | - |
Bos taurus | ADP + UMP | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
3% loss of activity after 10 min | Bos taurus |
100 | - |
dry uridine kinase precipitated with Pb2+, 30% loss of activity after 1 h, dry uridine kinase precipitated with Zn2+, 25% loss of activity after 1 h | Bos taurus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 7.5 | complete loss of activity below pH 4.0 | Bos taurus |