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Literature summary for 2.7.1.48 extracted from

  • Liacouras, A.S.; Anderson, E.P.
    Uridine-cytidine kinase. Purification from a murine neoplasm and characterization of the enzyme (1975), Arch. Biochem. Biophys., 168, 66-73.
    View publication on PubMed
Search for more literature for 2.7.1.48

General Stability

General Stability Organism
complete loss of activity after storage at -18°C for 24 h in the presence of 0.1 mM dithiothreitol Mus musculus
to achieve stability phosphate buffers are used throughout purification and for storage of enzyme fractions Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 13 mM, 66% of activity with Mg2+ Mus musculus
Fe2+ can partially replace Mg2+ Mus musculus
Fe2+ 13 mM, 65% of activity with Mg2+ Mus musculus
Mg2+ required for full activity Mus musculus
Mg2+ maximal activity at 13 mM Mus musculus
Mn2+ can partially replace Mg2+ Mus musculus
Mn2+ 13 mM, 80% of activity with Mg2+ Mus musculus
additional information not activated by Ca2+ and Cu2+ Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
160000
-
 gel filtration Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-cellulose, ammonium sulfate, hydroxyapatite Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
mastocytoma cell P815 cell line Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.03
-
-
 Mus musculus

Storage Stability

Storage Stability Organism
-18°C, phosphate buffer, pH 7.4-8.2, several months, no loss of activity Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 5-azacytidine 39.1% of the activity with uridine Mus musculus ADP + 5-azacytidine 5'-monophosphate
-
 ?
ATP + 5-fluorouridine 66.9% of the activity with uridine Mus musculus ADP + 5-fluorouridine 5'-monophosphate
-
 ?
ATP + 6-azacytidine 60.4% of the activity with uridine Mus musculus ADP + 6-azacytidine 5'-monophosphate
-
 ?
ATP + 6-azauridine 65.5% of the activity with uridine Mus musculus ADP + 6-azauridine 5'-monophosphate
-
 ?
ATP + cytidine 50% of activity with uridine Mus musculus ADP + CMP
-
 ?
ATP + cytidine 35.6% of the activity with uridine Mus musculus ADP + CMP
-
 ?
ATP + uridine no activity with thymine riboside, deoxyribosides, cytosine arabinoside, adenosine, purine deoxyribosides, UMP, CMP and deoxyribosides Mus musculus ADP + UMP
-
 ?
ATP + uridine ATP most effective as phosphate donor Mus musculus ADP + UMP
-
 ?
dATP + uridine 20.5% of activity with ATP Mus musculus dADP + UMP
-
 ?
dCTP + uridine 49.7% of activity with ATP Mus musculus dCDP + UMP
-
 ?
dGTP + uridine 23.5% of activity with ATP Mus musculus dGDP + UMP
-
 ?
dUTP + uridine 66.7% of activity with ATP Mus musculus dUDP + UMP
-
 ?
GTP + uridine
-
 Mus musculus UMP + GDP
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7
-
 Mus musculus