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Literature summary for 2.7.1.48 extracted from

  • Orengo, A.; Saunders, G.F.
    Regulation of a thermostable pyrimidine ribonucleoside kinase by cytidine triphosphate (1972), Biochemistry, 11, 1761-1767.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
CTP 80-85% inhibition at approx. 2 mM Geobacillus stearothermophilus
CTP complete inhibition at approx. 2 mM Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.21
-
uridine
-
Geobacillus stearothermophilus
0.27
-
uridine 37°C Rattus norvegicus
0.64
-
uridine
-
Geobacillus stearothermophilus
1.6
-
uridine
-
Geobacillus stearothermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
109000
-
sucrose density gradient centrifugation Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
-
-
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
streptomycin, ammonium sulfate, calcium phosphate, DEAE-cellulose Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + uridine
-
Rattus norvegicus ADP + UMP
-
?
ATP + uridine
-
Geobacillus stearothermophilus ADP + UMP
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
higher activity than at 10°C and 37°C Geobacillus stearothermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
59.5
-
no loss of activity after 30 min Geobacillus stearothermophilus
59.5
-
79% loss of activity after 4 min Rattus norvegicus