BRENDA - Enzyme Database
show all sequences of 2.7.1.47

Purification and properties of D-ribulokinase and D-xylulokinase from Klebsiella aerogenes

Neuberger, M.S.; Hartley, B.S.; Walker, J.E.; Biochem. J. 193, 513-524 (1981)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli K12 strain NC629 harbouring multi-copy lambdaprbt-101 prophage which carries the rbt-operon
Klebsiella aerogenes
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.4
-
D-ribulose
pH 7.8, 28C
Klebsiella aerogenes
140
-
D-arabitol
pH 7.8, 28C
Klebsiella aerogenes
220
-
ribitol
pH 7.8, 28C
Klebsiella aerogenes
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
60000
-
2 * 60000, recombinant enzyme expressed in Escherichia coli strain NC629, SDS-PAGE
Klebsiella aerogenes
112000
-
recombinant enzyme expressed in E. coli strain NC629, gel filtration
Klebsiella aerogenes
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Klebsiella aerogenes
-
expressed as constitutive enzyme in Escherichia coli K12 construct strain NC629
-
Purification (Commentary)
Commentary
Organism
-
Klebsiella aerogenes
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
84
-
-
Klebsiella aerogenes
Storage Stability
Storage Stability
Organism
-20C, in 50% v/v glycerol, in the dark, 1 month
Klebsiella aerogenes
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + D-arabitol
poor substrate, not L-isomer
641637
Klebsiella aerogenes
?
-
-
-
?
ATP + D-ribulose
-
641637
Klebsiella aerogenes
ADP + D-ribulose 5-phosphate
-
641637
Klebsiella aerogenes
?
ATP + ribitol
poor substrate
641637
Klebsiella aerogenes
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
dimer
2 * 60000, recombinant enzyme expressed in Escherichia coli strain NC629, SDS-PAGE
Klebsiella aerogenes
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli K12 strain NC629 harbouring multi-copy lambdaprbt-101 prophage which carries the rbt-operon
Klebsiella aerogenes
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.4
-
D-ribulose
pH 7.8, 28C
Klebsiella aerogenes
140
-
D-arabitol
pH 7.8, 28C
Klebsiella aerogenes
220
-
ribitol
pH 7.8, 28C
Klebsiella aerogenes
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
60000
-
2 * 60000, recombinant enzyme expressed in Escherichia coli strain NC629, SDS-PAGE
Klebsiella aerogenes
112000
-
recombinant enzyme expressed in E. coli strain NC629, gel filtration
Klebsiella aerogenes
Purification (Commentary) (protein specific)
Commentary
Organism
-
Klebsiella aerogenes
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
84
-
-
Klebsiella aerogenes
Storage Stability (protein specific)
Storage Stability
Organism
-20C, in 50% v/v glycerol, in the dark, 1 month
Klebsiella aerogenes
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + D-arabitol
poor substrate, not L-isomer
641637
Klebsiella aerogenes
?
-
-
-
?
ATP + D-ribulose
-
641637
Klebsiella aerogenes
ADP + D-ribulose 5-phosphate
-
641637
Klebsiella aerogenes
?
ATP + ribitol
poor substrate
641637
Klebsiella aerogenes
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 60000, recombinant enzyme expressed in Escherichia coli strain NC629, SDS-PAGE
Klebsiella aerogenes
Other publictions for EC 2.7.1.47
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
641637
Neuberger
Purification and properties of ...
Klebsiella aerogenes
Biochem. J.
193
513-524
1981
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1
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3
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2
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3
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1
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1
1
3
1
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1
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3
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2
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1
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1
1
3
1
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-
-
-
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-
641636
Stayton
Purification, properties, and ...
Klebsiella aerogenes
J. Biol. Chem.
254
3765-3771
1979
1
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2
2
1
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1
2
1
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2
-
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1
1
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1
1
2
1
-
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-
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-
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-
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1
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-
-
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2
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2
-
1
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1
2
1
-
-
-
1
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1
1
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
641635
Fromm
D-Ribulokinase from Aerobacter ...
Klebsiella aerogenes
J. Biol. Chem.
234
3097-3101
1959
1
-
-
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1
2
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3
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1
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2
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1
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1
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3
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1
1
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1
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1
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2
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3
-
1
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1
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1
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3
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-
-
-
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1
1
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-
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