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Literature summary for 2.7.1.47 extracted from

  • Neuberger, M.S.; Hartley, B.S.; Walker, J.E.
    Purification and properties of D-ribulokinase and D-xylulokinase from Klebsiella aerogenes (1981), Biochem. J., 193, 513-524.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli K12 strain NC629 harbouring multi-copy lambdaprbt-101 prophage which carries the rbt-operon Klebsiella aerogenes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
D-ribulose pH 7.8, 28°C Klebsiella aerogenes
140
-
D-arabitol pH 7.8, 28°C Klebsiella aerogenes
220
-
ribitol pH 7.8, 28°C Klebsiella aerogenes

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
2 * 60000, recombinant enzyme expressed in Escherichia coli strain NC629, SDS-PAGE Klebsiella aerogenes
112000
-
recombinant enzyme expressed in E. coli strain NC629, gel filtration Klebsiella aerogenes

Organism

Organism UniProt Comment Textmining
Klebsiella aerogenes
-
expressed as constitutive enzyme in Escherichia coli K12 construct strain NC629
-

Purification (Commentary)

Purification (Comment) Organism
-
Klebsiella aerogenes

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
84
-
-
Klebsiella aerogenes

Storage Stability

Storage Stability Organism
-20°C, in 50% v/v glycerol, in the dark, 1 month Klebsiella aerogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-arabitol poor substrate, not L-isomer Klebsiella aerogenes ?
-
?
ATP + D-ribulose
-
Klebsiella aerogenes ADP + D-ribulose 5-phosphate
-
?
ATP + ribitol poor substrate Klebsiella aerogenes ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 60000, recombinant enzyme expressed in Escherichia coli strain NC629, SDS-PAGE Klebsiella aerogenes