BRENDA - Enzyme Database show
show all sequences of 2.7.1.47

D-Ribulokinase from Aerobacter aerogenes

Fromm, H.J.; J. Biol. Chem. 234, 3097-3101 (1959)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
2-mercaptoethanol
activation
Klebsiella aerogenes
Inhibitors
Inhibitors
Commentary
Organism
Structure
Mg2+
inhibition at a Mg/ATP ratio above 2, 11% inhibition at a ratio of 3
Klebsiella aerogenes
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.83
-
ATP
pH 7.7, 27°C
Klebsiella aerogenes
0.92
-
D-ribulose
pH 7.7, 37°C
Klebsiella aerogenes
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
activation, can replace Mg2+ with 23% efficiency
Klebsiella aerogenes
Mg2+
optimum Mg/ATP-ratio: 1-2, inhibits at higher values
Klebsiella aerogenes
Mn2+
activation, can replace Mg2+ with 54% efficiency
Klebsiella aerogenes
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + D-ribulose
Klebsiella aerogenes
inducible enzyme of ribitol catabolism
?
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Klebsiella aerogenes
-
-
-
Purification (Commentary)
Commentary
Organism
partial
Klebsiella aerogenes
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Klebsiella aerogenes
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + D-ribulose
specific for D-isomer
641635
Klebsiella aerogenes
ADP + D-ribulose 5-phosphate
-
641635
Klebsiella aerogenes
?
ATP + D-ribulose
ATP cannot be replaced by ITP, GTP or ADP
641635
Klebsiella aerogenes
ADP + D-ribulose 5-phosphate
-
641635
Klebsiella aerogenes
?
ATP + D-ribulose
inducible enzyme of ribitol catabolism
641635
Klebsiella aerogenes
?
-
-
-
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
9.5
broad
Klebsiella aerogenes
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
10.5
pH 6.0: about 70% of maximal activity, pH 10.5: about half-maximal activity at pH 10.5
Klebsiella aerogenes
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
2-mercaptoethanol
activation
Klebsiella aerogenes
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Mg2+
inhibition at a Mg/ATP ratio above 2, 11% inhibition at a ratio of 3
Klebsiella aerogenes
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.83
-
ATP
pH 7.7, 27°C
Klebsiella aerogenes
0.92
-
D-ribulose
pH 7.7, 37°C
Klebsiella aerogenes
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
activation, can replace Mg2+ with 23% efficiency
Klebsiella aerogenes
Mg2+
optimum Mg/ATP-ratio: 1-2, inhibits at higher values
Klebsiella aerogenes
Mn2+
activation, can replace Mg2+ with 54% efficiency
Klebsiella aerogenes
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + D-ribulose
Klebsiella aerogenes
inducible enzyme of ribitol catabolism
?
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Klebsiella aerogenes
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Klebsiella aerogenes
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + D-ribulose
specific for D-isomer
641635
Klebsiella aerogenes
ADP + D-ribulose 5-phosphate
-
641635
Klebsiella aerogenes
?
ATP + D-ribulose
ATP cannot be replaced by ITP, GTP or ADP
641635
Klebsiella aerogenes
ADP + D-ribulose 5-phosphate
-
641635
Klebsiella aerogenes
?
ATP + D-ribulose
inducible enzyme of ribitol catabolism
641635
Klebsiella aerogenes
?
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
9.5
broad
Klebsiella aerogenes
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
10.5
pH 6.0: about 70% of maximal activity, pH 10.5: about half-maximal activity at pH 10.5
Klebsiella aerogenes
Other publictions for EC 2.7.1.47
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
641637
Neuberger
Purification and properties of ...
Klebsiella aerogenes
Biochem. J.
193
513-524
1981
-
-
1
-
-
-
-
3
-
-
2
-
-
3
-
-
1
-
-
-
1
1
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
3
-
-
2
-
-
-
-
1
-
-
1
1
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
641636
Stayton
Purification, properties, and ...
Klebsiella aerogenes
J. Biol. Chem.
254
3765-3771
1979
1
-
-
-
-
2
2
1
-
1
2
1
-
2
-
-
1
1
-
-
1
1
2
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
2
-
1
-
1
2
1
-
-
-
1
-
-
1
1
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
641635
Fromm
D-Ribulokinase from Aerobacter ...
Klebsiella aerogenes
J. Biol. Chem.
234
3097-3101
1959
1
-
-
-
-
-
1
2
-
3
-
1
-
2
-
-
1
-
-
-
1
-
3
-
-
-
-
-
1
1
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
2
-
3
-
1
-
-
-
1
-
-
1
-
3
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-