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show all sequences of 2.7.1.45

Crystallization and preliminary crystallographic analysis of 2-keto-3-deoxygluconate kinase from Thermus thermophilus

Inagaki, E.; Ukita, Y.; Kumei, M.; Kajihara, Y.; Tahirov, T.H.; Acta Crystallogr. Sect. D 60, 761-763 (2004)

Data extracted from this reference:

Crystallization (Commentary)
Crystallization
Organism
purified recombinant enzyme, free enzyme or in complex with ATP and 2-dehydro-3-deoxy-D-gluconate, method 1: automated microbatch method, 500 nl 23 mg/ml protein mixed with 500 nl screen solution containing 44% v/v methylpentanediol, 10% v/v dioxane, 0.1 M Na HEPES, pH 7.7, and covered with 0.015 ml silicone and parraffin oil mixture, 18°C, crystals grow to final dimensions within 1 month after appearance, method 2: sitting drop vapour diffusion method at 25°C, 0.001 ml 10 mg/ml protein solution mixed with equal volume of reservoir solution and equilibrated against 0.1 ml reservoir solution containing 28% v/v methylpentanediol, 10 mM CaCl2, 0.1 M trisodium citrate buffer, pH 5.6, or for cocrystallization of enzyme with ligands containing 0.35-0.45 M ammonium sulfate and 0.1 M Tris-HCl, pH 8.5, X-ray diffraction structure determination and analysis at 2.1-3.2 A resolution
Thermus thermophilus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + 2-dehydro-3-deoxy-D-gluconate
Thermus thermophilus
-
ADP + 6-phospho-2-dehydro-3-deoxy-D-gluconate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Thermus thermophilus
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + 2-dehydro-3-deoxy-D-gluconate
-
657469
Thermus thermophilus
ADP + 6-phospho-2-dehydro-3-deoxy-D-gluconate
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
ATP
-
Thermus thermophilus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
ATP
-
Thermus thermophilus
Crystallization (Commentary) (protein specific)
Crystallization
Organism
purified recombinant enzyme, free enzyme or in complex with ATP and 2-dehydro-3-deoxy-D-gluconate, method 1: automated microbatch method, 500 nl 23 mg/ml protein mixed with 500 nl screen solution containing 44% v/v methylpentanediol, 10% v/v dioxane, 0.1 M Na HEPES, pH 7.7, and covered with 0.015 ml silicone and parraffin oil mixture, 18°C, crystals grow to final dimensions within 1 month after appearance, method 2: sitting drop vapour diffusion method at 25°C, 0.001 ml 10 mg/ml protein solution mixed with equal volume of reservoir solution and equilibrated against 0.1 ml reservoir solution containing 28% v/v methylpentanediol, 10 mM CaCl2, 0.1 M trisodium citrate buffer, pH 5.6, or for cocrystallization of enzyme with ligands containing 0.35-0.45 M ammonium sulfate and 0.1 M Tris-HCl, pH 8.5, X-ray diffraction structure determination and analysis at 2.1-3.2 A resolution
Thermus thermophilus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + 2-dehydro-3-deoxy-D-gluconate
Thermus thermophilus
-
ADP + 6-phospho-2-dehydro-3-deoxy-D-gluconate
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + 2-dehydro-3-deoxy-D-gluconate
-
657469
Thermus thermophilus
ADP + 6-phospho-2-dehydro-3-deoxy-D-gluconate
-
-
-
?
Other publictions for EC 2.7.1.45
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738267
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1
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1
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1
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1
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1
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3
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1
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1
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1
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676988
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6
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5
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1
-
12
2
1
2
4
2
1
1
2
1
-
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2
1
-
-
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-
-
-
7
-
12
7
-
-
-
-
2
-
-
1
-
12
2
2
2
6
4
2
1
2
-
-
-
-
-
-
-
695053
Mathews
-
Crystal structure of 2-keto-3- ...
Thermotoga maritima
Proteins Struct. Funct. Bioinform.
70
603-608
2007
-
-
1
1
-
-
-
-
-
-
-
-
-
1
-
-
1
1
-
-
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-
-
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-
-
1
-
1
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1
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-
-
-
-
672718
Jung
-
Identification and characteriz ...
Thermoplasma acidophilum
Biotechnol. Bioprocess Eng.
10
535-539
2005
-
-
-
-
-
1
-
-
-
-
3
-
-
1
-
1
1
-
-
-
1
-
2
1
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
3
-
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-
1
1
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-
1
-
2
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
657469
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Crystallization and preliminar ...
Thermus thermophilus
Acta Crystallogr. Sect. D
60
761-763
2004
-
-
-
1
-
-
-
-
-
-
-
1
-
2
-
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1
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1
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1
1
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1
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1
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659714
Ohshima
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J. Mol. Biol.
340
477-489
2004
-
-
-
1
-
-
-
2
-
3
1
-
-
93
-
-
1
1
-
-
-
-
6
2
1
-
-
2
1
-
-
1
-
-
-
-
-
-
1
1
-
-
-
-
-
2
-
3
1
-
-
-
-
1
-
-
-
-
6
2
1
-
-
2
1
-
-
-
-
-
-
-
-
-
636961
Pouyssegur
Study of the common degradativ ...
Escherichia coli
Biochimie
53
771-781
1971
-
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1
-
-
-
-
1
-
-
-
1
-
1
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-
1
-
-
-
-
-
3
-
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-
1
-
1
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1
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1
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1
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1
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3
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1
-
1
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-
636960
Cynkin
Uronic acid metabolism in bact ...
Escherichia coli, Klebsiella aerogenes, no activity in Azobacter agilis, no activity in Neurospora crassa, no activity in Salmonella typhimurium
J. Biol. Chem.
235
1576-1579
1969
-
-
-
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-
1
1
-
3
-
-
-
5
-
-
1
-
-
-
1
1
10
-
-
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-
-
1
1
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-
-
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-
-
-
1
-
1
-
3
-
-
-
-
-
1
-
-
1
1
10
-
-
-
-
-
1
1
-
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