BRENDA - Enzyme Database
show all sequences of 2.7.1.44

Identification of galacturonic acid-1-phosphate kinase, a new member of the GHMP kinase superfamily in plants, and comparison with galactose-1-phosphate kinase

Yang, T.; Bar-Peled, L.; Gebhart, L.; Lee, S.G.; Bar-Peled, M.; J. Biol. Chem. 284, 21526-21535 (2009)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
His-tag, expressed in Escherichia coli BL21(de3)plysS
Arabidopsis thaliana
Engineering
Protein Variants
Commentary
Organism
A386S
possibly critical role in catalysis, relative activity: 90% (wild-type: 100%)
Arabidopsis thaliana
A41E
possibly critical for sugar binding, relative activity: 0% (wild-type: 100%)
Arabidopsis thaliana
Y250F
possibly important for substrate specificity (galacturonic acid /glucuronic acid), relative activity: 90% (wild-type: 100%), 50% activity with D-glucuronic acid (wild-type: 0%)
Arabidopsis thaliana
Inhibitors
Inhibitors
Commentary
Organism
Structure
ADP
addition (4 mM) results in 40% reduction of activity
Arabidopsis thaliana
EDTA
-
Arabidopsis thaliana
additional information
no inhibition is observed by CTP, GTP ITP,TTP, or UTP (4 mM)
Arabidopsis thaliana
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0708
-
D-galacturonate
wild-type protein
Arabidopsis thaliana
0.195
-
ATP
wild-type protein
Arabidopsis thaliana
0.395
-
D-galacturonate
Y250F mutant protein
Arabidopsis thaliana
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
Mn2+ (32% relative activity) and Ca2+ (61% relative activity) can substitute for Mg2+
Arabidopsis thaliana
Organism
Organism
UniProt
Commentary
Textmining
Arabidopsis thaliana
Q8VYG2
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Arabidopsis thaliana
Source Tissue
Source Tissue
Commentary
Organism
Textmining
flower
-
Arabidopsis thaliana
-
leaf
-
Arabidopsis thaliana
-
root
-
Arabidopsis thaliana
-
silique
-
Arabidopsis thaliana
-
stem
-
Arabidopsis thaliana
-
Storage Stability
Storage Stability
Organism
-20C, crude recombinant enzyme is stable
Arabidopsis thaliana
-20C, purified enzyme preparations lose more than 90% of activity within one week
Arabidopsis thaliana
-80C, stable for several months when diluted with glycerol to 25% (v/v) and flash-frozen in liquid nitrogen
Arabidopsis thaliana
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
D-galacturonate + ATP
wild-type protein: no activity with D-galactose, D-glucose, D-fructose, D-mannose, D-gluconic acid, D-xylose, N-acetyl-D-galactosamine, N-acetyl-D-glucosamine, D-glucosamine, D-arabinose, L-arabinose, L-rhamnose,or L-Fucose, these monosaccharides (4 mM) have no effect on activity in the presence of galacturonic acid, neither CTP, GTP ITP,TTP, nor UTP are substrates
704516
Arabidopsis thaliana
1-phospho-alpha-D-galacturonate + ADP
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
alpha-D-galacturonic acid kinase
-
Arabidopsis thaliana
alpha-D-galacturonic acid-1-phosphate kinase
-
Arabidopsis thaliana
GalAK
-
Arabidopsis thaliana
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
-
Arabidopsis thaliana
Temperature Range [C]
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
4
42
-
Arabidopsis thaliana
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
55
-
no activity at 55C
Arabidopsis thaliana
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
7.8
-
Arabidopsis thaliana
pH Range
pH Minimum
pH Maximum
Commentary
Organism
3.3
9.5
-
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
Commentary
Organism
His-tag, expressed in Escherichia coli BL21(de3)plysS
Arabidopsis thaliana
Engineering (protein specific)
Protein Variants
Commentary
Organism
A386S
possibly critical role in catalysis, relative activity: 90% (wild-type: 100%)
Arabidopsis thaliana
A41E
possibly critical for sugar binding, relative activity: 0% (wild-type: 100%)
Arabidopsis thaliana
Y250F
possibly important for substrate specificity (galacturonic acid /glucuronic acid), relative activity: 90% (wild-type: 100%), 50% activity with D-glucuronic acid (wild-type: 0%)
Arabidopsis thaliana
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
ADP
addition (4 mM) results in 40% reduction of activity
Arabidopsis thaliana
EDTA
-
Arabidopsis thaliana
additional information
no inhibition is observed by CTP, GTP ITP,TTP, or UTP (4 mM)
Arabidopsis thaliana
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0708
-
D-galacturonate
wild-type protein
Arabidopsis thaliana
0.195
-
ATP
wild-type protein
Arabidopsis thaliana
0.395
-
D-galacturonate
Y250F mutant protein
Arabidopsis thaliana
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
Mn2+ (32% relative activity) and Ca2+ (61% relative activity) can substitute for Mg2+
Arabidopsis thaliana
Purification (Commentary) (protein specific)
Commentary
Organism
-
Arabidopsis thaliana
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
flower
-
Arabidopsis thaliana
-
leaf
-
Arabidopsis thaliana
-
root
-
Arabidopsis thaliana
-
silique
-
Arabidopsis thaliana
-
stem
-
Arabidopsis thaliana
-
Storage Stability (protein specific)
Storage Stability
Organism
-20C, crude recombinant enzyme is stable
Arabidopsis thaliana
-20C, purified enzyme preparations lose more than 90% of activity within one week
Arabidopsis thaliana
-80C, stable for several months when diluted with glycerol to 25% (v/v) and flash-frozen in liquid nitrogen
Arabidopsis thaliana
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
D-galacturonate + ATP
wild-type protein: no activity with D-galactose, D-glucose, D-fructose, D-mannose, D-gluconic acid, D-xylose, N-acetyl-D-galactosamine, N-acetyl-D-glucosamine, D-glucosamine, D-arabinose, L-arabinose, L-rhamnose,or L-Fucose, these monosaccharides (4 mM) have no effect on activity in the presence of galacturonic acid, neither CTP, GTP ITP,TTP, nor UTP are substrates
704516
Arabidopsis thaliana
1-phospho-alpha-D-galacturonate + ADP
-
-
-
?
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
-
Arabidopsis thaliana
Temperature Range [C] (protein specific)
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
4
42
-
Arabidopsis thaliana
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
55
-
no activity at 55C
Arabidopsis thaliana
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
7.8
-
Arabidopsis thaliana
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
3.3
9.5
-
Arabidopsis thaliana
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.76
-
Alpha-D-glucuronic acid
Y250F mutant protein
Arabidopsis thaliana
6.3
-
ATP
wild-type protein
Arabidopsis thaliana
24
-
D-galacturonate
Y250F mutant protein
Arabidopsis thaliana
36
-
D-galacturonate
wild-type protein
Arabidopsis thaliana
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.76
-
Alpha-D-glucuronic acid
Y250F mutant protein
Arabidopsis thaliana
6.3
-
ATP
wild-type protein
Arabidopsis thaliana
24
-
D-galacturonate
Y250F mutant protein
Arabidopsis thaliana
36
-
D-galacturonate
wild-type protein
Arabidopsis thaliana
Other publictions for EC
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
704516
Yang
Identification of galacturonic ...
Arabidopsis thaliana
J. Biol. Chem.
284
21526-21535
2009
-
-
1
-
3
-
3
3
-
1
-
-
-
4
-
-
1
-
-
5
-
3
1
-
3
1
1
1
-
1
1
-
-
-
-
-
-
-
1
-
-
3
-
-
3
-
3
-
1
-
-
-
-
-
1
-
5
-
3
1
-
1
1
1
-
1
1
-
-
-
-
-
-
4
4
637498
Neufeld
Phosphorylation of D-galacturo ...
Fagopyrum esculentum, Petroselinum crispum, Pisum sativum, Raphanus sativus, Vigna radiata var. radiata
J. Biol. Chem.
236
3102-3105
1961
-
-
-
-
-
5
-
-
-
15
-
5
-
5
-
-
5
-
-
5
-
5
15
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
15
-
5
-
-
-
5
-
5
-
5
15
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-