BRENDA - Enzyme Database
show all sequences of 2.7.1.44

Phosphorylation of D-galacturonic acid by extracts from germinating seeds of Phaseolus aureus

Neufeld, E.F.; Feingold, D.S.; Ilves, S.M.; Kessler, G.; Hassid, W.Z.; J. Biol. Chem. 236, 3102-3105 (1961)

Data extracted from this reference:

General Stability
General Stability
Organism
mercaptoethanol, 0.05 M, is necessary for preservation of activity in dialysis buffer, lowering its concentration to 0.01 M reduces enzyme activity by 30 to 50%, omission of mercaptoethanol results in complete loss of activity
Vigna radiata var. radiata
mercaptoethanol, 0.05 M, is necessary for preservation of activity in dialysis buffer, lowering its concentration to 0.01 M reduces enzyme activity by 30 to 50%, omission of mercaptoethanol results in complete loss of activity
Pisum sativum
mercaptoethanol, 0.05 M, is necessary for preservation of activity in dialysis buffer, lowering its concentration to 0.01 M reduces enzyme activity by 30 to 50%, omission of mercaptoethanol results in complete loss of activity
Petroselinum crispum
mercaptoethanol, 0.05 M, is necessary for preservation of activity in dialysis buffer, lowering its concentration to 0.01 M reduces enzyme activity by 30 to 50%, omission of mercaptoethanol results in complete loss of activity
Raphanus sativus
mercaptoethanol, 0.05 M, is necessary for preservation of activity in dialysis buffer, lowering its concentration to 0.01 M reduces enzyme activity by 30 to 50%, omission of mercaptoethanol results in complete loss of activity
Fagopyrum esculentum
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Co2+
divalent metal ion is required, lower activity than Mg2+ or Mn2+
Fagopyrum esculentum
Co2+
divalent metal ion is required, lower activity than Mg2+ or Mn2+
Petroselinum crispum
Co2+
divalent metal ion is required, lower activity than Mg2+ or Mn2+
Pisum sativum
Co2+
divalent metal ion is required, lower activity than Mg2+ or Mn2+
Raphanus sativus
Co2+
divalent metal ion is required, lower activity than Mg2+ or Mn2+
Vigna radiata var. radiata
Mg2+
divalent metal ion is required, Zn2+ or Ni2+ are ineffective
Fagopyrum esculentum
Mg2+
divalent metal ion is required, Zn2+ or Ni2+ are ineffective
Petroselinum crispum
Mg2+
divalent metal ion is required, Zn2+ or Ni2+ are ineffective
Pisum sativum
Mg2+
divalent metal ion is required, Zn2+ or Ni2+ are ineffective
Raphanus sativus
Mg2+
divalent metal ion is required, Zn2+ or Ni2+ are ineffective
Vigna radiata var. radiata
Mn2+
divalent metal ion is required, higher activity than Mg2+. Zn2+ or Ni2+ are ineffective
Fagopyrum esculentum
Mn2+
divalent metal ion is required, higher activity than Mg2+. Zn2+ or Ni2+ are ineffective
Petroselinum crispum
Mn2+
divalent metal ion is required, higher activity than Mg2+. Zn2+ or Ni2+ are ineffective
Pisum sativum
Mn2+
divalent metal ion is required, higher activity than Mg2+. Zn2+ or Ni2+ are ineffective
Raphanus sativus
Mn2+
divalent metal ion is required, higher activity than Mg2+. Zn2+ or Ni2+ are ineffective
Vigna radiata var. radiata
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
ATP + D-galacturonate
Vigna radiata var. radiata
involved in formation of pectin
ADP + 1-phospho-alpha-D-galacturonate
-
-
-
ATP + D-galacturonate
Pisum sativum
involved in formation of pectin
ADP + 1-phospho-alpha-D-galacturonate
-
-
-
ATP + D-galacturonate
Petroselinum crispum
involved in formation of pectin
ADP + 1-phospho-alpha-D-galacturonate
-
-
-
ATP + D-galacturonate
Raphanus sativus
involved in formation of pectin
ADP + 1-phospho-alpha-D-galacturonate
-
-
-
ATP + D-galacturonate
Fagopyrum esculentum
involved in formation of pectin
ADP + 1-phospho-alpha-D-galacturonate
-
-
-
Organism
Organism
UniProt
Commentary
Textmining
Fagopyrum esculentum
-
buckwheat
-
Petroselinum crispum
-
parsley
-
Pisum sativum
-
pea
-
Raphanus sativus
-
radish
-
Vigna radiata var. radiata
-
mung bean
-
Purification (Commentary)
Purification (Commentary)
Organism
partial
Fagopyrum esculentum
partial
Petroselinum crispum
partial
Pisum sativum
partial
Raphanus sativus
partial
Vigna radiata var. radiata
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Petroselinum crispum
-
root
-
Raphanus sativus
-
seed
germinating
Fagopyrum esculentum
-
seed
germinating
Pisum sativum
-
seed
germinating
Vigna radiata var. radiata
-
Storage Stability
Storage Stability
Organism
4°C, 0.05 M mercaptoethanol, stable up to 10 days
Vigna radiata var. radiata
4°C, 0.05 M mercaptoethanol, stable up to 10 days
Pisum sativum
4°C, 0.05 M mercaptoethanol, stable up to 10 days
Petroselinum crispum
4°C, 0.05 M mercaptoethanol, stable up to 10 days
Raphanus sativus
4°C, 0.05 M mercaptoethanol, stable up to 10 days
Fagopyrum esculentum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
ATP + D-galacturonate
-
637498
Vigna radiata var. radiata
ADP + 1-phospho-alpha-D-galacturonate
-
637498
Vigna radiata var. radiata
?
ATP + D-galacturonate
-
637498
Pisum sativum
ADP + 1-phospho-alpha-D-galacturonate
-
637498
Pisum sativum
?
ATP + D-galacturonate
-
637498
Petroselinum crispum
ADP + 1-phospho-alpha-D-galacturonate
-
637498
Petroselinum crispum
?
ATP + D-galacturonate
-
637498
Raphanus sativus
ADP + 1-phospho-alpha-D-galacturonate
-
637498
Raphanus sativus
?
ATP + D-galacturonate
-
637498
Fagopyrum esculentum
ADP + 1-phospho-alpha-D-galacturonate
-
637498
Fagopyrum esculentum
?
ATP + D-galacturonate
involved in formation of pectin
637498
Vigna radiata var. radiata
ADP + 1-phospho-alpha-D-galacturonate
-
-
-
-
ATP + D-galacturonate
involved in formation of pectin
637498
Pisum sativum
ADP + 1-phospho-alpha-D-galacturonate
-
-
-
-
ATP + D-galacturonate
involved in formation of pectin
637498
Petroselinum crispum
ADP + 1-phospho-alpha-D-galacturonate
-
-
-
-
ATP + D-galacturonate
involved in formation of pectin
637498
Raphanus sativus
ADP + 1-phospho-alpha-D-galacturonate
-
-
-
-
ATP + D-galacturonate
involved in formation of pectin
637498
Fagopyrum esculentum
ADP + 1-phospho-alpha-D-galacturonate
-
-
-
-
additional information
D-glucuronic acid, D-galactose and L-arabinose are no substrates, ATP cannot be replaced by other nucleotides
637498
Vigna radiata var. radiata
?
-
-
-
-
additional information
D-glucuronic acid, D-galactose and L-arabinose are no substrates, ATP cannot be replaced by other nucleotides
637498
Pisum sativum
?
-
-
-
-
additional information
D-glucuronic acid, D-galactose and L-arabinose are no substrates, ATP cannot be replaced by other nucleotides
637498
Petroselinum crispum
?
-
-
-
-
additional information
D-glucuronic acid, D-galactose and L-arabinose are no substrates, ATP cannot be replaced by other nucleotides
637498
Raphanus sativus
?
-
-
-
-
additional information
D-glucuronic acid, D-galactose and L-arabinose are no substrates, ATP cannot be replaced by other nucleotides
637498
Fagopyrum esculentum
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Fagopyrum esculentum
37
-
assay at
Petroselinum crispum
37
-
assay at
Pisum sativum
37
-
assay at
Raphanus sativus
37
-
assay at
Vigna radiata var. radiata
General Stability (protein specific)
General Stability
Organism
mercaptoethanol, 0.05 M, is necessary for preservation of activity in dialysis buffer, lowering its concentration to 0.01 M reduces enzyme activity by 30 to 50%, omission of mercaptoethanol results in complete loss of activity
Vigna radiata var. radiata
mercaptoethanol, 0.05 M, is necessary for preservation of activity in dialysis buffer, lowering its concentration to 0.01 M reduces enzyme activity by 30 to 50%, omission of mercaptoethanol results in complete loss of activity
Pisum sativum
mercaptoethanol, 0.05 M, is necessary for preservation of activity in dialysis buffer, lowering its concentration to 0.01 M reduces enzyme activity by 30 to 50%, omission of mercaptoethanol results in complete loss of activity
Petroselinum crispum
mercaptoethanol, 0.05 M, is necessary for preservation of activity in dialysis buffer, lowering its concentration to 0.01 M reduces enzyme activity by 30 to 50%, omission of mercaptoethanol results in complete loss of activity
Raphanus sativus
mercaptoethanol, 0.05 M, is necessary for preservation of activity in dialysis buffer, lowering its concentration to 0.01 M reduces enzyme activity by 30 to 50%, omission of mercaptoethanol results in complete loss of activity
Fagopyrum esculentum
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Co2+
divalent metal ion is required, lower activity than Mg2+ or Mn2+
Fagopyrum esculentum
Co2+
divalent metal ion is required, lower activity than Mg2+ or Mn2+
Petroselinum crispum
Co2+
divalent metal ion is required, lower activity than Mg2+ or Mn2+
Pisum sativum
Co2+
divalent metal ion is required, lower activity than Mg2+ or Mn2+
Raphanus sativus
Co2+
divalent metal ion is required, lower activity than Mg2+ or Mn2+
Vigna radiata var. radiata
Mg2+
divalent metal ion is required, Zn2+ or Ni2+ are ineffective
Fagopyrum esculentum
Mg2+
divalent metal ion is required, Zn2+ or Ni2+ are ineffective
Petroselinum crispum
Mg2+
divalent metal ion is required, Zn2+ or Ni2+ are ineffective
Pisum sativum
Mg2+
divalent metal ion is required, Zn2+ or Ni2+ are ineffective
Raphanus sativus
Mg2+
divalent metal ion is required, Zn2+ or Ni2+ are ineffective
Vigna radiata var. radiata
Mn2+
divalent metal ion is required, higher activity than Mg2+. Zn2+ or Ni2+ are ineffective
Fagopyrum esculentum
Mn2+
divalent metal ion is required, higher activity than Mg2+. Zn2+ or Ni2+ are ineffective
Petroselinum crispum
Mn2+
divalent metal ion is required, higher activity than Mg2+. Zn2+ or Ni2+ are ineffective
Pisum sativum
Mn2+
divalent metal ion is required, higher activity than Mg2+. Zn2+ or Ni2+ are ineffective
Raphanus sativus
Mn2+
divalent metal ion is required, higher activity than Mg2+. Zn2+ or Ni2+ are ineffective
Vigna radiata var. radiata
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
ATP + D-galacturonate
Vigna radiata var. radiata
involved in formation of pectin
ADP + 1-phospho-alpha-D-galacturonate
-
-
-
ATP + D-galacturonate
Pisum sativum
involved in formation of pectin
ADP + 1-phospho-alpha-D-galacturonate
-
-
-
ATP + D-galacturonate
Petroselinum crispum
involved in formation of pectin
ADP + 1-phospho-alpha-D-galacturonate
-
-
-
ATP + D-galacturonate
Raphanus sativus
involved in formation of pectin
ADP + 1-phospho-alpha-D-galacturonate
-
-
-
ATP + D-galacturonate
Fagopyrum esculentum
involved in formation of pectin
ADP + 1-phospho-alpha-D-galacturonate
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Fagopyrum esculentum
partial
Petroselinum crispum
partial
Pisum sativum
partial
Raphanus sativus
partial
Vigna radiata var. radiata
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Petroselinum crispum
-
root
-
Raphanus sativus
-
seed
germinating
Fagopyrum esculentum
-
seed
germinating
Pisum sativum
-
seed
germinating
Vigna radiata var. radiata
-
Storage Stability (protein specific)
Storage Stability
Organism
4°C, 0.05 M mercaptoethanol, stable up to 10 days
Vigna radiata var. radiata
4°C, 0.05 M mercaptoethanol, stable up to 10 days
Pisum sativum
4°C, 0.05 M mercaptoethanol, stable up to 10 days
Petroselinum crispum
4°C, 0.05 M mercaptoethanol, stable up to 10 days
Raphanus sativus
4°C, 0.05 M mercaptoethanol, stable up to 10 days
Fagopyrum esculentum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
ATP + D-galacturonate
-
637498
Vigna radiata var. radiata
ADP + 1-phospho-alpha-D-galacturonate
-
637498
Vigna radiata var. radiata
?
ATP + D-galacturonate
-
637498
Pisum sativum
ADP + 1-phospho-alpha-D-galacturonate
-
637498
Pisum sativum
?
ATP + D-galacturonate
-
637498
Petroselinum crispum
ADP + 1-phospho-alpha-D-galacturonate
-
637498
Petroselinum crispum
?
ATP + D-galacturonate
-
637498
Raphanus sativus
ADP + 1-phospho-alpha-D-galacturonate
-
637498
Raphanus sativus
?
ATP + D-galacturonate
-
637498
Fagopyrum esculentum
ADP + 1-phospho-alpha-D-galacturonate
-
637498
Fagopyrum esculentum
?
ATP + D-galacturonate
involved in formation of pectin
637498
Vigna radiata var. radiata
ADP + 1-phospho-alpha-D-galacturonate
-
-
-
-
ATP + D-galacturonate
involved in formation of pectin
637498
Pisum sativum
ADP + 1-phospho-alpha-D-galacturonate
-
-
-
-
ATP + D-galacturonate
involved in formation of pectin
637498
Petroselinum crispum
ADP + 1-phospho-alpha-D-galacturonate
-
-
-
-
ATP + D-galacturonate
involved in formation of pectin
637498
Raphanus sativus
ADP + 1-phospho-alpha-D-galacturonate
-
-
-
-
ATP + D-galacturonate
involved in formation of pectin
637498
Fagopyrum esculentum
ADP + 1-phospho-alpha-D-galacturonate
-
-
-
-
additional information
D-glucuronic acid, D-galactose and L-arabinose are no substrates, ATP cannot be replaced by other nucleotides
637498
Vigna radiata var. radiata
?
-
-
-
-
additional information
D-glucuronic acid, D-galactose and L-arabinose are no substrates, ATP cannot be replaced by other nucleotides
637498
Pisum sativum
?
-
-
-
-
additional information
D-glucuronic acid, D-galactose and L-arabinose are no substrates, ATP cannot be replaced by other nucleotides
637498
Petroselinum crispum
?
-
-
-
-
additional information
D-glucuronic acid, D-galactose and L-arabinose are no substrates, ATP cannot be replaced by other nucleotides
637498
Raphanus sativus
?
-
-
-
-
additional information
D-glucuronic acid, D-galactose and L-arabinose are no substrates, ATP cannot be replaced by other nucleotides
637498
Fagopyrum esculentum
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Fagopyrum esculentum
37
-
assay at
Petroselinum crispum
37
-
assay at
Pisum sativum
37
-
assay at
Raphanus sativus
37
-
assay at
Vigna radiata var. radiata
Other publictions for EC 2.7.1.44
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
704516
Yang
Identification of galacturonic ...
Arabidopsis thaliana
J. Biol. Chem.
284
21526-21535
2009
-
-
1
-
3
-
3
3
-
1
-
-
-
4
-
-
1
-
-
5
-
3
1
-
3
1
1
1
-
1
1
-
-
-
-
-
-
-
1
-
-
3
-
-
3
-
3
-
1
-
-
-
-
-
1
-
5
-
3
1
-
1
1
1
-
1
1
-
-
-
-
-
-
4
4
637498
Neufeld
Phosphorylation of D-galacturo ...
Fagopyrum esculentum, Petroselinum crispum, Pisum sativum, Raphanus sativus, Vigna radiata var. radiata
J. Biol. Chem.
236
3102-3105
1961
-
-
-
-
-
5
-
-
-
15
-
5
-
5
-
-
5
-
-
5
-
5
15
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
15
-
5
-
-
-
5
-
5
-
5
15
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-