Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of a frameshift mutations in the major expressed isoform glucuronokinase 1 to potentially redirect metabolites to ascorbic acid (AsA). But radiotracer experiments with 3H-myo-inositol reveal that the mutants in glucuronokinase 1 accumulate only glucuronic acid and incorporate less metabolite into cell wall polymers. AsA is not labelled, suggesting that Arabidopsis thaliana cannot efficiently use glucuronic acid for AsA biosynthesis. All four mutants in glucuronokinase as well as the wild type have the same level of AsA in leaves. Construction of CRISPR/Cas9 knockouts, mutants of GlcAK1 are generated using CRISPR/Cas9 technology including transformation by the Agrobacterium tumefaciens (strain GV3101) system, resulting in mutants glcak1-3 and glcak1-4. Analysis of T-DNA insertional mutants glcak1-1 and glcak1-2 | Arabidopsis thaliana |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-glucuronate | Arabidopsis thaliana | - |
ADP + 1-phospho-alpha-D-glucuronate | - |
? | |
ATP + D-glucuronate | Arabidopsis thaliana Col-0 | - |
ADP + 1-phospho-alpha-D-glucuronate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q93ZC9 | - |
- |
Arabidopsis thaliana Col-0 | Q93ZC9 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Arabidopsis thaliana | - |
seedling | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-glucuronate | - |
Arabidopsis thaliana | ADP + 1-phospho-alpha-D-glucuronate | - |
? | |
ATP + D-glucuronate | - |
Arabidopsis thaliana Col-0 | ADP + 1-phospho-alpha-D-glucuronate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
At3g01640 | - |
Arabidopsis thaliana |
GlcAK1 | - |
Arabidopsis thaliana |
glucuronokinase1 | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
malfunction | GlcAK1 knockout mutant phenotype of T-DNA insertional mutants glcak1-1, glcak1-2, glcak1-3, and glcak1-4, overview | Arabidopsis thaliana |
metabolism | one alternative pathway for ascorbic acid (AsA) synthesis, which is similar to the biosynthesis route in mammals, is controversially discussed for plants. Pathway overview for AsA biosynthesis in plants, overview. Here, myo-inositol is cleaved to glucuronic acid and then converted via L-gulonate to AsA. In contrast to animals, plants have an effective recycling pathway for glucuronic acid, being a competitor for the metabolic rate. Recycling involves a phosphorylation at C1 by the enzyme glucuronokinase | Arabidopsis thaliana |