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Literature summary for 2.7.1.43 extracted from

  • Ivanov Kavkova, E.; Bloechl, C.; Tenhaken, R.
    The Myo-inositol pathway does not contribute to ascorbic acid synthesis (2019), Plant Biol., 21 Suppl 1, 95-102 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
additional information generation of a frameshift mutations in the major expressed isoform glucuronokinase 1 to potentially redirect metabolites to ascorbic acid (AsA). But radiotracer experiments with 3H-myo-inositol reveal that the mutants in glucuronokinase 1 accumulate only glucuronic acid and incorporate less metabolite into cell wall polymers. AsA is not labelled, suggesting that Arabidopsis thaliana cannot efficiently use glucuronic acid for AsA biosynthesis. All four mutants in glucuronokinase as well as the wild type have the same level of AsA in leaves. Construction of CRISPR/Cas9 knockouts, mutants of GlcAK1 are generated using CRISPR/Cas9 technology including transformation by the Agrobacterium tumefaciens (strain GV3101) system, resulting in mutants glcak1-3 and glcak1-4. Analysis of T-DNA insertional mutants glcak1-1 and glcak1-2 Arabidopsis thaliana

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + D-glucuronate Arabidopsis thaliana
-
ADP + 1-phospho-alpha-D-glucuronate
-
?
ATP + D-glucuronate Arabidopsis thaliana Col-0
-
ADP + 1-phospho-alpha-D-glucuronate
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q93ZC9
-
-
Arabidopsis thaliana Col-0 Q93ZC9
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Arabidopsis thaliana
-
seedling
-
Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-glucuronate
-
Arabidopsis thaliana ADP + 1-phospho-alpha-D-glucuronate
-
?
ATP + D-glucuronate
-
Arabidopsis thaliana Col-0 ADP + 1-phospho-alpha-D-glucuronate
-
?

Synonyms

Synonyms Comment Organism
At3g01640
-
Arabidopsis thaliana
GlcAK1
-
Arabidopsis thaliana
glucuronokinase1
-
Arabidopsis thaliana

General Information

General Information Comment Organism
malfunction GlcAK1 knockout mutant phenotype of T-DNA insertional mutants glcak1-1, glcak1-2, glcak1-3, and glcak1-4, overview Arabidopsis thaliana
metabolism one alternative pathway for ascorbic acid (AsA) synthesis, which is similar to the biosynthesis route in mammals, is controversially discussed for plants. Pathway overview for AsA biosynthesis in plants, overview. Here, myo-inositol is cleaved to glucuronic acid and then converted via L-gulonate to AsA. In contrast to animals, plants have an effective recycling pathway for glucuronic acid, being a competitor for the metabolic rate. Recycling involves a phosphorylation at C1 by the enzyme glucuronokinase Arabidopsis thaliana