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Literature summary for 2.7.1.42 extracted from

  • Katagiri, H.; Yamada, H.; Imai, K.
    On the transphosphorylation reactions catalyzed by glucose-1-phosphate phosphotransferase of Escherichia coli (1959), J. Biochem., 46, 1119-1126.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
(NH4)2MoO4 0.5%: 100% inhibition Escherichia coli
EDTA 2 mM: 3% inhibition Escherichia coli
fructose 0.2 M: 24% inhibition Escherichia coli
glucose 0.2 M: 84% inhibition Escherichia coli
KH2AsO4 5 mM: 25% inhibition Escherichia coli
KHSO4 5 mM: 13% inhibition, 50 mM: 100% inhibition Escherichia coli
maltose 0.2 M: 20% inhibition Escherichia coli
MgSO4 0.1 mM: 7% inhibition Escherichia coli
NaF 25 mM, 100% inhibition Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alpha-D-glucose 1-phosphate + riboflavin Escherichia coli
-
alpha-D-glucose + FMN
-
?
additional information Escherichia coli may be involved in biosynthesis of phosphate compounds that are essential for bacterial growth ?
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
neapolitanus, strain B-25
-

Purification (Commentary)

Purification (Comment) Organism
partial Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.12
-
pH 5.4, 30°C Escherichia coli

Storage Stability

Storage Stability Organism
0°C, 1 week, stable Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-D-glucose 1-phosphate + riboflavin
-
Escherichia coli alpha-D-glucose + FMN
-
?
beta-D-glucose 1-phosphate + riboflavin about 50% of activity compared to alpha-glucose 1-phosphate Escherichia coli beta-D-glucose + FMN
-
?
D-glucose 1-phosphate + 6-methyl-9-(D-1'-ribityl)-isoalloxazine 93% of activity compared to riboflavin Escherichia coli D-glucose + 6-methyl-9-(D-1'-ribityl)-isoalloxazine 5'-phosphate
-
?
D-glucose 1-phosphate + araboflavin 93% of activity compared to riboflavin Escherichia coli D-glucose + araboflavin 5'-phosphate
-
?
D-glucose 1-phosphate + isoriboflavin 73% of activity compared to riboflavin Escherichia coli D-glucose + isoriboflavin 5'-phosphate
-
?
additional information ATP, diphosphate, adenylate, glucose 6-phosphate, fructose 1,6-diphosphate, riboflavin ketoic acid and hydroxyethylisoalloxazine are no substrates Escherichia coli ?
-
?
additional information may be involved in biosynthesis of phosphate compounds that are essential for bacterial growth Escherichia coli ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3
-
broad Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
4 8 75% of maximal activity at pH 4, 20% of maximal activity at pH 8 Escherichia coli