Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(NH4)2MoO4 | 0.5%: 100% inhibition | Escherichia coli | |
EDTA | 2 mM: 3% inhibition | Escherichia coli | |
fructose | 0.2 M: 24% inhibition | Escherichia coli | |
glucose | 0.2 M: 84% inhibition | Escherichia coli | |
KH2AsO4 | 5 mM: 25% inhibition | Escherichia coli | |
KHSO4 | 5 mM: 13% inhibition, 50 mM: 100% inhibition | Escherichia coli | |
maltose | 0.2 M: 20% inhibition | Escherichia coli | |
MgSO4 | 0.1 mM: 7% inhibition | Escherichia coli | |
NaF | 25 mM, 100% inhibition | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-D-glucose 1-phosphate + riboflavin | Escherichia coli | - |
alpha-D-glucose + FMN | - |
? | |
additional information | Escherichia coli | may be involved in biosynthesis of phosphate compounds that are essential for bacterial growth | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
neapolitanus, strain B-25 | - |
Purification (Comment) | Organism |
---|---|
partial | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.12 | - |
pH 5.4, 30°C | Escherichia coli |
Storage Stability | Organism |
---|---|
0°C, 1 week, stable | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-D-glucose 1-phosphate + riboflavin | - |
Escherichia coli | alpha-D-glucose + FMN | - |
? | |
beta-D-glucose 1-phosphate + riboflavin | about 50% of activity compared to alpha-glucose 1-phosphate | Escherichia coli | beta-D-glucose + FMN | - |
? | |
D-glucose 1-phosphate + 6-methyl-9-(D-1'-ribityl)-isoalloxazine | 93% of activity compared to riboflavin | Escherichia coli | D-glucose + 6-methyl-9-(D-1'-ribityl)-isoalloxazine 5'-phosphate | - |
? | |
D-glucose 1-phosphate + araboflavin | 93% of activity compared to riboflavin | Escherichia coli | D-glucose + araboflavin 5'-phosphate | - |
? | |
D-glucose 1-phosphate + isoriboflavin | 73% of activity compared to riboflavin | Escherichia coli | D-glucose + isoriboflavin 5'-phosphate | - |
? | |
additional information | ATP, diphosphate, adenylate, glucose 6-phosphate, fructose 1,6-diphosphate, riboflavin ketoic acid and hydroxyethylisoalloxazine are no substrates | Escherichia coli | ? | - |
? | |
additional information | may be involved in biosynthesis of phosphate compounds that are essential for bacterial growth | Escherichia coli | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.3 | - |
broad | Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 8 | 75% of maximal activity at pH 4, 20% of maximal activity at pH 8 | Escherichia coli |