Activating Compound | Comment | Organism | Structure |
---|---|---|---|
fructose 1,6-bisphosphate | Ka of liver enzyme is 0.029 mM, of anoxic liver enzyme 0.0032 mM | Trachemys scripta elegans | |
additional information | L-aspartate has no significant effect on the activity of the liver enzyme at 0-20 mM | Trachemys scripta elegans | |
additional information | neither L-aspartate nor fructose 1,6-bisphosphate has an influence on muscle pyruvate kinase in terms of activation | Trachemys scripta elegans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Trachemys scripta elegans | |
KCl | - |
Trachemys scripta elegans | |
L-alanine | - |
Trachemys scripta elegans | |
Lactate | - |
Trachemys scripta elegans | |
additional information | no inhibiton by L-alanine | Trachemys scripta elegans | |
Urea | - |
Trachemys scripta elegans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
phosphoenolpyruvate | muscle enzyme, pH 7.2, 22°C | Trachemys scripta elegans | |
0.029 | - |
phosphoenolpyruvate | muscle enzyme, pH 6.6, 22°C | Trachemys scripta elegans | |
0.051 | - |
phosphoenolpyruvate | liver enzyme, pH 6.6, 22°C | Trachemys scripta elegans | |
0.057 | - |
phosphoenolpyruvate | anoxic muscle enzyme, pH 6.6, 22°C | Trachemys scripta elegans | |
0.061 | - |
phosphoenolpyruvate | anoxic muscle enzyme, pH 7.2, 22°C | Trachemys scripta elegans | |
0.092 | - |
phosphoenolpyruvate | anoxic liver enzyme, pH 6.6, 22°C | Trachemys scripta elegans | |
0.12 | - |
ADP | liver enzyme, pH 7.2, 22°C | Trachemys scripta elegans | |
0.13 | - |
ADP | anoxic liver enzyme, pH 7.2, 22°C | Trachemys scripta elegans | |
0.2 | - |
ADP | muscle enzyme, pH 7.2, 22°C | Trachemys scripta elegans | |
0.28 | - |
ADP | anoxic muscle enzyme, pH 7.2, 22°C | Trachemys scripta elegans | |
0.31 | - |
phosphoenolpyruvate | liver enzyme, pH 7.2, 22°C | Trachemys scripta elegans | |
0.55 | - |
phosphoenolpyruvate | anoxic liver enzyme, pH 7.2, 22°C | Trachemys scripta elegans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Trachemys scripta elegans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + phosphoenolpyruvate | Trachemys scripta elegans | - |
ATP + pyruvate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trachemys scripta elegans | Q9I8C5 | - |
- |
Trachemys scripta elegans | Q9PU23 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
alkylation | lysine methylation increases dramatically in the anoxic state by 70% relative to the control condition | Trachemys scripta elegans |
alkylation | lysine methylation of muscle PK increases significantly by 43% in the anoxic state compared to control | Trachemys scripta elegans |
phosphoprotein | anoxic red muscle PK shows a 42% increase in threonine phosphorylation relative to the control. But there are no significant differences in neither tyrosine nor serine residue phosphorylation between the control and anoxic states | Trachemys scripta elegans |
phosphoprotein | liver PK shows 60% increased threonine phosphorylation levels in the anoxic state compared to control. Conversely, phosphorylation on tyrosine residues decreases by 26% for anoxic PK relative to control, whereas serine phosphorylation is unchanged | Trachemys scripta elegans |
Purification (Comment) | Organism |
---|---|
native enzyme from liver by two different steps of cation exchange chromatography, 7.3fold | Trachemys scripta elegans |
native enzyme from red muscle by cation exchange chromatography, 82.8-97.6fold to homogeneity | Trachemys scripta elegans |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | liver-specific isozyme | Trachemys scripta elegans | - |
muscle | muscle-specific isozyme | Trachemys scripta elegans | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.49 | 1.32 | purified native liver enzyme, pH 7.2, 22°C | Trachemys scripta elegans |
11.8 | 14.5 | purified native muscle enzyme, pH 7.2, 22°C | Trachemys scripta elegans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + phosphoenolpyruvate | - |
Trachemys scripta elegans | ATP + pyruvate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 57000, SDS-PAGE | Trachemys scripta elegans |
Synonyms | Comment | Organism |
---|---|---|
liver PK | - |
Trachemys scripta elegans |
muscle PK | - |
Trachemys scripta elegans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at | Trachemys scripta elegans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Trachemys scripta elegans |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.01 | - |
anoxic liver enzyme, pH 7.2, 22°C | Trachemys scripta elegans | L-alanine | |
0.1 | - |
liver enzyme, pH 7.2, 22°C | Trachemys scripta elegans | L-alanine | |
1.1 | - |
anoxic liver enzyme, pH 7.2, 22°C, in presence of fructose 1,6-bisphosphate | Trachemys scripta elegans | ATP | |
2 | - |
liver enzyme, pH 7.2, 22°C, in presence of fructose 1,6-bisphosphate | Trachemys scripta elegans | ATP | |
2.5 | - |
liver enzyme, pH 7.2, 22°C | Trachemys scripta elegans | ATP | |
2.7 | - |
anoxic liver enzyme, pH 7.2, 22°C | Trachemys scripta elegans | ATP | |
7.9 | - |
anoxic muscle enzyme, pH 7.2, 22°C | Trachemys scripta elegans | ATP | |
11.6 | - |
muscle enzyme, pH 7.2, 22°C | Trachemys scripta elegans | ATP | |
34.4 | - |
muscle enzyme, pH 7.2, 22°C | Trachemys scripta elegans | Lactate | |
51 | - |
anoxic muscle enzyme, pH 7.2, 22°C | Trachemys scripta elegans | KCl | |
54 | - |
muscle enzyme, pH 7.2, 22°C | Trachemys scripta elegans | KCl | |
131 | - |
anoxic liver enzyme, pH 7.2, 22°C | Trachemys scripta elegans | Lactate | |
177.6 | - |
anoxic muscle enzyme, pH 7.2, 22°C | Trachemys scripta elegans | Lactate | |
187 | - |
liver enzyme, pH 7.2, 22°C | Trachemys scripta elegans | Lactate | |
410 | - |
anoxic liver enzyme, pH 7.2, 22°C | Trachemys scripta elegans | KCl | |
433 | - |
liver enzyme, pH 7.2, 22°C | Trachemys scripta elegans | KCl | |
1406 | - |
anoxic muscle enzyme, pH 7.2, 22°C | Trachemys scripta elegans | Urea | |
1410 | - |
liver enzyme, pH 7.2, 22°C | Trachemys scripta elegans | Urea | |
1901 | - |
muscle enzyme, pH 7.2, 22°C | Trachemys scripta elegans | Urea | |
1980 | - |
anoxic liver enzyme, pH 7.2, 22°C | Trachemys scripta elegans | Urea |