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Literature summary for 2.7.1.40 extracted from
- Expression, purification, and characterization of pyruvate kinase from Mycobacterium tuberculosis a key allosteric regulatory enzyme (2018), Int. J. Mycobacteriol., 7, 368-374 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene pyk, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Mycobacterium tuberculosis |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Mycobacterium tuberculosis |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + phosphoenolpyruvate | Mycobacterium tuberculosis | - |
ATP + pyruvate | - |
? | |
| ADP + phosphoenolpyruvate | Mycobacterium tuberculosis H37Rv | - |
ATP + pyruvate | - |
? | |
| ADP + phosphoenolpyruvate | Mycobacterium tuberculosis ATCC 25618 | - |
ATP + pyruvate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | P9WKE5 | - |
- |
| Mycobacterium tuberculosis ATCC 25618 | P9WKE5 | - |
- |
| Mycobacterium tuberculosis H37Rv | P9WKE5 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Mycobacterium tuberculosis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5.31 | - |
purified recombinant His-tagged enzyme, pH 7.6, 37°C | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + phosphoenolpyruvate | - |
Mycobacterium tuberculosis | ATP + pyruvate | - |
? | |
| ADP + phosphoenolpyruvate | - |
Mycobacterium tuberculosis H37Rv | ATP + pyruvate | - |
? | |
| ADP + phosphoenolpyruvate | - |
Mycobacterium tuberculosis ATCC 25618 | ATP + pyruvate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 54000, recombinant enzyme, SDS-PAGE | Mycobacterium tuberculosis |
| More | secondary structure analysis by circular dichroism spectroscopy revealing a content of 17% alpha-helix, 34% beta-sheet, and 49% turns in the enzyme | Mycobacterium tuberculosis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PYK | - |
Mycobacterium tuberculosis |
| Rv1617 | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Mycobacterium tuberculosis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
assay at | Mycobacterium tuberculosis |
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Release 2023.1 (January 2023)
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