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Literature summary for 2.7.1.40 extracted from

  • Mandal, S.; Bose, P.; Dawar, S.; Rajarani, A.; Santha, I.
    Kinetic and expression profiling of cytosolic pyruvate kinase enzyme during seed development of Indian mustard (Brassica juncea) (2011), J. Plant Biochem. Biotechnol., 20, 102-109.
No PubMed abstract available
Search for more literature for 2.7.1.40

Cloned(Commentary)

Cloned (Comment) Organism
expression profiling by RT-PCR techniques Brassica juncea

Inhibitors

Inhibitors Comment Organism Structure
3-phosphoglycerate 8% inhibition at 5 mM Brassica juncea
AMP 11% inhibition at 5 mM Brassica juncea
ATP 48% inhibition at 5 mM, significant allosteric effector of the partially purified enzyme Brassica juncea
citrate 60% inhibition at 5 mM, significant allosteric effector of the partially purified enzyme Brassica juncea
isocitrate 30% inhibition at 5 mM Brassica juncea
additional information poor inhibition of glucose 1-phosphate and fumaric acid at 5 mM Brassica juncea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics, in presence of saturated substrate concentration, the enzyme exhibits hyperbolic kinetics for both ADP and PEP Brassica juncea
0.0965
-
 ADP pH 7.0, 25°C Brassica juncea
0.1785
-
 phosphoenolpyruvate pH 7.0, 25°C Brassica juncea

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast plastidial isozyme Brassica juncea 9507
-
cytoplasm cytosolic isozyme Brassica juncea 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Brassica juncea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + pyruvate Brassica juncea
-
 ADP + phosphoenolpyruvate
-
 r
ATP + pyruvate Brassica juncea Pusa Bold
-
 ADP + phosphoenolpyruvate
-
 r

Organism

Organism UniProt Comment Textmining
Brassica juncea
-
-
-
Brassica juncea Pusa Bold
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native cytosolic isozyme 12.3fold from seeds by anion exchange and hydroxyapatite chromatography, inactivation of plastidial isoform by heating the enzyme extract at 60°C for 3 min Brassica juncea

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
 Brassica juncea
-
leaf
-
 Brassica juncea
-
seed expression profiling of cytosolic pyruvate kinase enzyme during seed development, enzyme activity is highest during middle stage, overview Brassica juncea
-
seedling highest activity Brassica juncea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
690.7
-
 partially purified cytosolic isozyme, pH 7.0, 25°C Brassica juncea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + pyruvate
-
 Brassica juncea ADP + phosphoenolpyruvate
-
 r
ATP + pyruvate
-
 Brassica juncea Pusa Bold ADP + phosphoenolpyruvate
-
 r

Synonyms

Synonyms Comment Organism
PYK
-
 Brassica juncea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Brassica juncea

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 3 min, pH 7.0, plastidial isoform, inactivation Brassica juncea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 narrow optimum, cytosolic isozyme Brassica juncea
8
-
 plastidial isozyme Brassica juncea

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Brassica juncea

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.64
-
 pH 7.0, 25°C Brassica juncea citrate
5
-
 pH 7.0, 25°C Brassica juncea ATP

General Information

General Information Comment Organism
metabolism pyruvate kinase is a crucial regulatory enzyme involved in glycolysis Brassica juncea