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Literature summary for 2.7.1.40 extracted from

  • Akhtar, K.; Gupta, V.; Koul, A.; Alam, N.; Bhat, R.; Bamezai, R.N.
    Differential behavior of missense mutations in the intersubunit contact domain of the human pyruvate kinase M2 isozyme (2009), J. Biol. Chem., 284, 11971-11981.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.40

Activating Compound

Activating Compound Comment Organism Structure
D-fructose 1,6-bisphosphate 20.5% increase of activity at 2.5 mM Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
H391Y pyruvate kinase M2 isozyme missense mutation found in cells from Bloom syndrome patients prone to develop cancer, the mutant protein maintains its homotetrameric structure, similar to the wild type protein, but shows a loss of activity of 20%, the mutant shows a 6fold increase in affinity for phosphoenolpyruvate and behaves like a non-allosteric protein with compromised cooperative binding Homo sapiens
K422R pyruvate kinase M2 isozyme missense mutation found in cells from Bloom syndrome patients prone to develop cancer, the mutant protein maintains its homotetrameric structure, similar to the wild type protein, but shows a loss of activity of 75%, the affinity for phosphoenolpyruvate is lost significantly in K422R Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
L-Phe 23.5% activity left at 1 mM L-Phe in the absence of D-fructose 1,6-bisphosphate Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P14618
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and DEAE-Sepharose column chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + phosphoenolpyruvate
-
 Homo sapiens ATP + pyruvate
-
 ?

Subunits

Subunits Comment Organism
homotetramer
-
 Homo sapiens

Synonyms

Synonyms Comment Organism
pyruvate kinase M2 isozyme Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
 the wild type enzyme and mutant enzyme K422R shows complete loss of activity after 15 min at 4°C, the mutant enzyme H391Y shows 36% loss of activity after 15 min at 4°C Homo sapiens
37
-
 the wild type enzyme shows 56% loss of activity after 3 h at 37°C, the mutant enzyme K422R shows 80% loss of activity after 3 h at 37°C, the mutant enzyme H391Y shows 1% loss of activity after 3 h at 37°C Homo sapiens
60
-
 the wild type enzyme and mutant enzyme K422R show complete loss of activity after 10 min at 60°C, while mutant enzyme H391Y displays only 2% loss of activity after 10 min at 60°C Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ADP
-
 Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.067
-
 L-Phe mutant enzyme K422R, in the absence of D-fructose 1,6-bisphosphate Homo sapiens
0.162
-
 L-Phe mutant enzyme K422R, in the presence of 2.5 mM D-fructose 1,6-bisphosphate Homo sapiens
0.176
-
 L-Phe wild type enzyme, in the absence of D-fructose 1,6-bisphosphate Homo sapiens
0.261
-
 L-Phe wild type enzyme, in the presence of 2.5 mM D-fructose 1,6-bisphosphate Homo sapiens
1.431
-
 L-Phe mutant enzyme H391Y, in the absence of D-fructose 1,6-bisphosphate Homo sapiens
1.78
-
 L-Phe mutant enzyme H391Y, in the presence of 2.5 mM D-fructose 1,6-bisphosphate Homo sapiens