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Literature summary for 2.7.1.40 extracted from

  • Tulloch, L.B.; Morgan, H.P.; Hannaert, V.; Michels, P.A.; Fothergill-Gilmore, L.A.; Walkinshaw, M.D.
    Sulphate removal induces a major conformational change in Leishmania mexicana pyruvate kinase in the crystalline state (2008), J. Mol. Biol., 383, 615-626.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
D-fructose 2,6-bisphosphate wild-type, S0.5 value 0.000082 mM Leishmania mexicana

Crystallization (Commentary)

Crystallization (Comment) Organism
crystals grown with ammonium sulfate as precipitant adopt an active-like conformation, with sulfate ions at the active and effector sites. Crystal soaking in sulfate-free buffers induces major conformational changes in the tetramer. The unwinding of the Aalpha6' helix and the inward hinge movement of the B domain are coupled with a significant widening of the tetramer caused by lateral movement of the C-domains Leishmania mexicana

Protein Variants

Protein Variants Comment Organism
D315N 3.7% of wild-type activity. S0.5 value for fructose 2,6-bisphosphate 0.001 mM Leishmania mexicana
D315S 1.4% of wild-type activity. S0.5 value for fructose 2,6-bisphosphate 0.00161 mM Leishmania mexicana
E451W 72% of wild-type activity. S0.5 value for fructose 2,6-bisphosphate 0.000177 mM Leishmania mexicana
S314N 69% of wild-type activity. S0.5 value for fructose 2,6-bisphosphate 0.000403 mM Leishmania mexicana
S314Q 48% of wild-type activity. S0.5 value for fructose 2,6-bisphosphate 0.000122 mM Leishmania mexicana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information S0.5 value of phosphoenolpyruvate is 1.06 mM for wild-type, 1.3 mM for mutant E451W Leishmania mexicana
0.214
-
ADP wild-type, pH 7.5 Leishmania mexicana
0.214
-
ADP mutant E451W, pH 7.5 Leishmania mexicana

Organism

Organism UniProt Comment Textmining
Leishmania mexicana Q27686
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + phosphoenolpyruvate
-
Leishmania mexicana ATP + pyruvate
-
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