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Literature summary for 2.7.1.40 extracted from

  • Suzuki, K.; Ito, S.; Shimizu-Ibuka, A.; Sakai, H.
    Crystallization and preliminary X-ray analysis of pyruvate kinase from Bacillus stearothermophilus (2005), Acta Crystallogr. Sect. F, 61, 759-761.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
D-ribose 5-phosphate
-
Geobacillus stearothermophilus
additional information not activated by fructose 1,6-bisphosphate Geobacillus stearothermophilus

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain PB25 Geobacillus stearothermophilus

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging and sitting drop vapour diffusion methods Geobacillus stearothermophilus

Protein Variants

Protein Variants Comment Organism
W416F/V435W high affinity for phosphoenolpyruvate compared to the wild-type enzyme, but its saturation curve is still sigmoidal and hyperbolic in the presence of allosteric activators Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Butyl-Toyopearl 650S chromatography and Resource Q column chromatography Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + phosphoenolpyruvate
-
Geobacillus stearothermophilus ATP + pyruvate
-
?

Cofactor

Cofactor Comment Organism Structure
ADP
-
Geobacillus stearothermophilus
AMP
-
Geobacillus stearothermophilus