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Literature summary for 2.7.1.40 extracted from

  • Papadopoulos, A.I.; Anagnostis, A.; Lazarou, D.
    Effect of insecticide injection on pyruvate kinase of the insect Tenebrio molitor (Coleopteran) (2005), Pestic. Biochem. Physiol., 82, 115-124.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
methyl paraoxon in larvae, low doses of methyl paraoxon and methyl parathion activatd the enzyme but as the dose increases the Km value returned to normal levels Tenebrio molitor
methyl parathion in larvae, low doses of methyl paraoxon and methyl parathion activated the enzyme but as the dose increases the Km value returned to normal levels Tenebrio molitor

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Km value of the enzyme towards phosphoenolpyruvate is higher in the pupae stage than in the rest developmental stages although the same enzyme type appears to be present throughout the life span of the insect. Starvation results to activation of the enzyme by increasing the Km value in the two feeding stages, namely larvae and adult, while injury has the opposite effect Tenebrio molitor
0.22
-
phosphoenolpyruvate enzyme from larva Tenebrio molitor
0.26
-
phosphoenolpyruvate enzyme from adult Tenebrio molitor
0.6
-
phosphoenolpyruvate enzyme from pupae Tenebrio molitor

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Tenebrio molitor 5829
-

Organism

Organism UniProt Comment Textmining
Tenebrio molitor
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Tenebrio molitor

Source Tissue

Source Tissue Comment Organism Textmining
adult
-
Tenebrio molitor
-
larva
-
Tenebrio molitor
-
pupa
-
Tenebrio molitor
-
whole body homogenate Tenebrio molitor
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
9.69
-
-
Tenebrio molitor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + phosphoenolpyruvate
-
Tenebrio molitor ATP + pyruvate
-
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