Activating Compound | Comment | Organism | Structure |
---|---|---|---|
AMP | allosteric activator | Thermotoga maritima |
Cloned (Comment) | Organism |
---|---|
- |
Pyrobaculum aerophilum |
- |
Aeropyrum pernix |
expression in Escherichia coli | Thermotoga maritima |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | allosteric inhibitor | Thermotoga maritima | |
KCl | 40 mM, 50-60% decrease in activity of recombinant enzyme | Thermotoga maritima | |
NH4Cl | 40 mM, 50-60% decrease in activity of recombinant enzyme | Thermotoga maritima |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | divalent cation required, at 1 mM Ca2+ activation results in 14% of the activity with Mg2+ | Aeropyrum pernix | |
Ca2+ | divalent cation required, at 1 mM Ca2+ activation results in 3% of the activity with Mg2+ | Thermotoga maritima | |
Ca2+ | divalent cation required, at 1 mM Ca2+ activation results in 7% of the activity with Mg2+ | Archaeoglobus fulgidus | |
Co2+ | divalent cation required, at 1 mM Co2+ activation results in 120% of the activity with Mg2+ | Thermotoga maritima | |
Co2+ | divalent cation required, at 1 mM Co2+ activation results in 170% of the activity with Mg2+ | Aeropyrum pernix | |
Co2+ | divalent cation required, at 1 mM Co2+ activation results in 80% of the activity with Mn2+ | Pyrobaculum aerophilum | |
Cu2+ | divalent cation required, at 1 mM Cu2+ activation results in 86% of the activity with Mg2+ | Archaeoglobus fulgidus | |
Mg2+ | divalent cation required | Thermotoga maritima | |
Mg2+ | divalent cation required | Aeropyrum pernix | |
Mg2+ | divalent cation required, at 1 mM highest activity with Mg2+ | Archaeoglobus fulgidus | |
Mn2+ | divalent cation required | Pyrobaculum aerophilum | |
Mn2+ | divalent cation required, at 1 mM Mn2+ activation results in 160% of the activity with Mg2+ | Aeropyrum pernix | |
Mn2+ | divalent cation required, at 1 mM Mn2+ activation results in 35% of the activity with Mg2+ | Thermotoga maritima | |
Mn2+ | divalent cation required, at 1 mM Mn2+ activation results in 63% of the activity with Mg2+ | Archaeoglobus fulgidus | |
additional information | 1 mM Mg2+ does not shows significant activity. No activity is observed with Ca2+ and Zn2+ | Thermotoga maritima | |
additional information | 1 mM Mg2+ does not shows significant activity. No activity is observed with Ca2+ and Zn2+ | Pyrobaculum aerophilum | |
Ni2+ | divalent cation required, at 1 mM Ni2+ activation results in 2% of the activity with Mg2+ | Archaeoglobus fulgidus | |
Zn2+ | divalent cation required, at 1 mM Zn2 activation results in 2.5% of the activity with Mg2+ | Thermotoga maritima | |
Zn2+ | divalent cation required, at 1 mM Zn2+ activation results in 11% of the activity with Mg2+ | Aeropyrum pernix | |
Zn2+ | divalent cation required, at 1 mM Zn2+ activation results in 6.5% of the activity with Mg2+ | Archaeoglobus fulgidus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
4 * 48000, SDS-PAGE | Pyrobaculum aerophilum |
49000 | - |
4 * 49000, SDS-PAGE | Archaeoglobus fulgidus |
51000 | - |
4 * 51000, SDS-PAGE | Aeropyrum pernix |
51000 | - |
4 * 51000, native enzyme, SDS-PAGE | Thermotoga maritima |
56000 | - |
4 * 56000, recombinant enzyme, SDS-PAGE | Thermotoga maritima |
190000 | - |
recombinant enzyme, gel filtration | Thermotoga maritima |
194000 | - |
native enzyme, gel filtration | Thermotoga maritima |
203000 | - |
gel filtration | Archaeoglobus fulgidus |
205000 | - |
gel filtration | Pyrobaculum aerophilum |
207000 | - |
gel filtration | Aeropyrum pernix |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aeropyrum pernix | - |
- |
- |
Archaeoglobus fulgidus | - |
- |
- |
Archaeoglobus fulgidus 7324 | - |
- |
- |
Pyrobaculum aerophilum | - |
- |
- |
Thermotoga maritima | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Archaeoglobus fulgidus |
- |
Thermotoga maritima |
- |
Pyrobaculum aerophilum |
- |
Aeropyrum pernix |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
320 | - |
- |
Thermotoga maritima |
1000 | - |
80°C | Archaeoglobus fulgidus |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 51000, SDS-PAGE | Aeropyrum pernix |
tetramer | 4 * 48000, SDS-PAGE | Pyrobaculum aerophilum |
tetramer | 4 * 49000, SDS-PAGE | Archaeoglobus fulgidus |
tetramer | 4 * 51000, native enzyme, SDS-PAGE | Thermotoga maritima |
tetramer | 4 * 56000, recombinant enzyme, SDS-PAGE | Thermotoga maritima |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
recombinant enzyme | Thermotoga maritima |
85 | - |
- |
Archaeoglobus fulgidus |
95 | - |
above | Aeropyrum pernix |
98 | - |
above | Pyrobaculum aerophilum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
120 min, stable | Archaeoglobus fulgidus |
90 | - |
half-life: 20 min | Archaeoglobus fulgidus |
100 | - |
half-life: 220 min | Pyrobaculum aerophilum |
100 | - |
120 min, stable | Aeropyrum pernix |
100 | - |
almost complete loss of activity after 7 min. Addition of 1 M (NH4)2SO4 effectively stabilizes the enzyme agianst heat inactivation, retaining about 50% residual activity after incubation at 120 min | Archaeoglobus fulgidus |
100 | - |
half-life: about 20 min, almost complete loss of activity after 120 min, native and recombinant enzyme, about 40% residual activity after 120 min incubation in presence of 1 M (NH4)2SO4 | Thermotoga maritima |
110 | - |
half-life: 30 min. (NH4)2SO4, NaCl or KCl, each at 1 mM, do not stabilize the enzyme agianst heat inactivation | Aeropyrum pernix |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.9 | - |
recombiannt enzyme | Thermotoga maritima |
6 | - |
- |
Pyrobaculum aerophilum |
6 | - |
native enzyme | Thermotoga maritima |
6.1 | - |
- |
Aeropyrum pernix |
6.6 | - |
- |
Archaeoglobus fulgidus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7 | about 30% of maximal activity at pH 5.5 and 7.0 | Thermotoga maritima |
5.5 | 7.5 | about 50% of maximal activity at pH 5.5 and pH 7.5 | Archaeoglobus fulgidus |
5.5 | 7.5 | about 50% of maximal activity at pH 5.5 and pH 7.5 | Aeropyrum pernix |