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Literature summary for 2.7.1.40 extracted from

  • Abe, K.; Yamada, T.
    Purification and properties of pyruvate kinase from Streptococcus mutans (1982), J. Bacteriol., 149, 299-305.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.40

Activating Compound

Activating Compound Comment Organism Structure
D-fructose 1,6-diphosphate not Streptococcus mutans
D-glucose 6-phosphate requirement Streptococcus mutans
D-ribose 5-phosphate activation Streptococcus mutans
D-Ribulose 1,5-diphosphate not Streptococcus mutans
glyceraldehyde 3-phosphate not Streptococcus mutans
additional information no activation by glucose 1,6-diphosphate, 5'-IMP, 2',3'-AMP, 2',3'-GMP, 2',3'-UMP Streptococcus mutans

General Stability

General Stability Organism
dialysis for 20 h against 40 mM Tris-HCl, Tris-maleate or morpholinepropanesulfonic acid-NaOH buffer, pH 7, inactivates, but stable to dialysis against phosphate buffer, pH 7 Streptococcus mutans
phosphate restores activity of dialyzed, partially purified, not fully purified, enzyme preparations Streptococcus mutans

Inhibitors

Inhibitors Comment Organism Structure
phosphate at high concentrations; strong Streptococcus mutans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic parameters Streptococcus mutans
0.026
-
 GDP pH 7.5 Streptococcus mutans
0.04 0.063 phosphoenolpyruvate pH 7.5 Streptococcus mutans
0.049 0.1 IDP
-
 Streptococcus mutans
0.22
-
 phosphoenolpyruvate in the presence of glucose 6-phosphate, at pH 7.5 Streptococcus mutans
0.25 0.26 GDP UDP, at pH 7.5 Streptococcus mutans
0.39
-
 ADP in the presence of glucose 6-phosphate, pH 7.5 Streptococcus mutans

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ not Streptococcus mutans
divalent cation requirement Streptococcus mutans
divalent cation requires both a divalent and a monovalent cation Streptococcus mutans
K+ requirement Streptococcus mutans
K+ only together with activating divalent cation Streptococcus mutans
Mg2+ requirement Streptococcus mutans
Mn2+ requirement Streptococcus mutans
Mn2+ only together with K+ Streptococcus mutans
monovalent cation requirement Streptococcus mutans
monovalent cation requires both a divalent and a monovalent cation Streptococcus mutans
additional information absolute requirement for a bivalent and a monovalent cation with Mg2+ and K+ fulfilling this Streptococcus mutans
additional information no activation by Li+ Streptococcus mutans
Na+ not Streptococcus mutans
NH4+ requirement Streptococcus mutans
NH4+ can replace K+ Streptococcus mutans
NH4+ only together with activating divalent cation Streptococcus mutans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
 4 * 44000, SDS-PAGE Streptococcus mutans
180000 190000 gel filtration Streptococcus mutans

Organism

Organism UniProt Comment Textmining
Streptococcus mutans
-
-
-
Streptococcus mutans JC2
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptococcus mutans

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Streptococcus mutans
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Streptococcus mutans

Storage Stability

Storage Stability Organism
4°C, in 40 mM potassium phosphate buffer, pH 7, 10 mM 2-mercaptoethanol, 3 months Streptococcus mutans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + phosphoenolpyruvate
-
 Streptococcus mutans ATP + pyruvate
-
 ?
ADP + phosphoenolpyruvate
-
 Streptococcus mutans JC2 ATP + pyruvate
-
 ?
CDP + phosphoenolpyruvate poor substrate Streptococcus mutans CTP + pyruvate
-
 ?
CDP + phosphoenolpyruvate less effective than ADP Streptococcus mutans CTP + pyruvate
-
 ?
CDP + phosphoenolpyruvate poor substrate Streptococcus mutans JC2 CTP + pyruvate
-
 ?
CDP + phosphoenolpyruvate less effective than ADP Streptococcus mutans JC2 CTP + pyruvate
-
 ?
GDP + phosphoenolpyruvate less effective than ADP Streptococcus mutans GTP + pyruvate
-
 ?
GDP + phosphoenolpyruvate less effective than ADP Streptococcus mutans JC2 GTP + pyruvate
-
 ?
IDP + phosphoenolpyruvate less effective than ADP Streptococcus mutans ITP + pyruvate
-
 ?
TDP + phosphoenolpyruvate poor substrate Streptococcus mutans TTP + pyruvate
-
 ?
UDP + phosphoenolpyruvate less effective than ADP Streptococcus mutans UTP + pyruvate
-
 ?
UDP + phosphoenolpyruvate less effective than ADP Streptococcus mutans JC2 UTP + pyruvate
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 44000, SDS-PAGE Streptococcus mutans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8 7.7
-
 Streptococcus mutans

Cofactor

Cofactor Comment Organism Structure
AMP not Streptococcus mutans
GMP not Streptococcus mutans