Activating Compound | Comment | Organism | Structure |
---|---|---|---|
D-fructose 1,6-diphosphate | activation | Salmonella enterica subsp. enterica serovar Typhimurium | |
D-fructose 1,6-diphosphate | kinetics | Salmonella enterica subsp. enterica serovar Typhimurium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.16 | 0.17 | ADP | isozyme II, 30°C, pH 6.8 | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.2 | 0.35 | ADP | enzyme form I | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.2 | 0.35 | ADP | 30°C, pH 6.8 | Salmonella enterica subsp. enterica serovar Typhimurium |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | requirement | Salmonella enterica subsp. enterica serovar Typhimurium | |
Mg2+ | positive cooperativity | Salmonella enterica subsp. enterica serovar Typhimurium | |
Mn2+ | requirement | Salmonella enterica subsp. enterica serovar Typhimurium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
4 * 47000, isozyme II, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
54000 | - |
4 * 54000, isozyme I, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
190000 | - |
isozyme PKII, gel filtration | Salmonella enterica subsp. enterica serovar Typhimurium |
220000 | - |
isozyme PKII, gel filtration | Salmonella enterica subsp. enterica serovar Typhimurium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica subsp. enterica serovar Typhimurium | - |
strain LT-2, highest activity in cells grown on glycolytic substrates, e.g. glucose, galactose, fructose or glycerol | - |
Purification (Comment) | Organism |
---|---|
2 isoforms, I and II | Salmonella enterica subsp. enterica serovar Typhimurium |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Salmonella enterica subsp. enterica serovar Typhimurium | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Storage Stability | Organism |
---|---|
4°C, in 50 mM Tris-HCl buffer, isozyme I up to 10 days, isozyme II 4 days | Salmonella enterica subsp. enterica serovar Typhimurium |
EDTA, 2-mercaptoethanol, dithiothreitol, glycerol and KCl stabilize, being more effective at refrigerated conditions than at room temperature | Salmonella enterica subsp. enterica serovar Typhimurium |
frozen, in 50 mM Tris-HCl buffer, isozyme I at least 9 months, isozyme II 4 months | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + phosphoenolpyruvate | positive cooperativity for phosphoenolpyruvate | Salmonella enterica subsp. enterica serovar Typhimurium | ATP + pyruvate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 54000, isozyme I, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
tetramer | 4 * 47000, isozyme II, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
at pH 7-8, stable | Salmonella enterica subsp. enterica serovar Typhimurium |
37 | - |
at pH 7-8, stable | Salmonella enterica subsp. enterica serovar Typhimurium |
55 | - |
15 min, 12% loss of activity, isozyme I, inactivation of isozyme II | Salmonella enterica subsp. enterica serovar Typhimurium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
more active in HEPES than in other buffers | Salmonella enterica subsp. enterica serovar Typhimurium |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 6.8 | increase of activity in this range, decline at higher pH-values | Salmonella enterica subsp. enterica serovar Typhimurium |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | at 25°C and 37°C, stable | Salmonella enterica subsp. enterica serovar Typhimurium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
AMP | activation | Salmonella enterica subsp. enterica serovar Typhimurium |