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Literature summary for 2.7.1.40 extracted from
- Purification and properties of pyruvate kinase from Mycobacterium smegmatis (1983), Arch. Biochem. Biophys., 225, 320-330.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| D-glucose 6-phosphate | activation | Mycolicibacterium smegmatis |  |
General Stability
| General Stability | Organism |
|---|---|
| 0.1 M KCl stabilizes enzyme in dilute solutions | Mycolicibacterium smegmatis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ATP | glucose 6-phosphate reverses | Mycolicibacterium smegmatis | |
| Ba2+ | in decreasing order of inhibitory efficiency: Ni2+, Zn2+, Cu2+, Ca2+, Ba2+ | Mycolicibacterium smegmatis | |
| Ca2+ | in decreasing order of inhibitory efficiency: Ni2+, Zn2+, Cu2+, Ca2+, Ba2+ | Mycolicibacterium smegmatis | |
| Cu2+ | in decreasing order of inhibitory efficiency: Ni2+, Zn2+, Cu2+, Ca2+, Ba2+ | Mycolicibacterium smegmatis | |
| Ni2+ | in decreasing order of inhibitory efficiency: Ni2+, Zn2+, Cu2+, Ca2+, Ba2+ | Mycolicibacterium smegmatis | |
| phosphate | - |
Mycolicibacterium smegmatis | |
| sulfate | - |
Mycolicibacterium smegmatis | |
| Zn2+ | in decreasing order of inhibitory efficiency: Ni2+, Zn2+, Cu2+, Ca2+, Ba2+ | Mycolicibacterium smegmatis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetic parameters | Mycolicibacterium smegmatis | |
| 0.04 | 0.063 | phosphoenolpyruvate | - |
Mycolicibacterium smegmatis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | not | Mycolicibacterium smegmatis | |
| Mg2+ | requirement | Mycolicibacterium smegmatis | |
| Mg2+ | kinetics | Mycolicibacterium smegmatis | |
| Mn2+ | requirement | Mycolicibacterium smegmatis | |
| Mn2+ | can partially replace Mg2+ | Mycolicibacterium smegmatis | |
| Mn2+ | kinetics | Mycolicibacterium smegmatis | |
| monovalent cation | not | Mycolicibacterium smegmatis | |
| additional information | no activation by Ba2+ | Mycolicibacterium smegmatis | |
| additional information | no activation by Cu2+, Ni2+ | Mycolicibacterium smegmatis | |
| Zn2+ | not | Mycolicibacterium smegmatis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 230700 | - |
gel filtration | Mycolicibacterium smegmatis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycolicibacterium smegmatis | - |
- |
- |
| Mycolicibacterium smegmatis CDC 46 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Mycolicibacterium smegmatis |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + pyruvate = ADP + phosphoenolpyruvate | allosteric enzyme: homotropic | Mycolicibacterium smegmatis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Mycolicibacterium smegmatis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 220 | - |
- |
Mycolicibacterium smegmatis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, in 50 mM phosphate buffer, pH 7, 100 mM KCl, 2 mM phosphoenolpyruvate, 0.1% 2-mercaptoethanol, 50% v/v glycerol, at least 6 months | Mycolicibacterium smegmatis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + phosphoenolpyruvate | best nucleoside diphosphate substrate | Mycolicibacterium smegmatis | ATP + pyruvate | - |
? | |
| ADP + phosphoenolpyruvate | positive cooperativity for phosphoenolpyruvate | Mycolicibacterium smegmatis | ATP + pyruvate | - |
? | |
| ADP + phosphoenolpyruvate | best nucleoside diphosphate substrate | Mycolicibacterium smegmatis CDC 46 | ATP + pyruvate | - |
? | |
| ADP + phosphoenolpyruvate | positive cooperativity for phosphoenolpyruvate | Mycolicibacterium smegmatis CDC 46 | ATP + pyruvate | - |
? | |
| CDP + phosphoenolpyruvate | less effective than ADP | Mycolicibacterium smegmatis | CTP + pyruvate | - |
? | |
| CDP + phosphoenolpyruvate | less effective than ADP | Mycolicibacterium smegmatis CDC 46 | CTP + pyruvate | - |
? | |
| additional information | allosteric enzyme: homotropic | Mycolicibacterium smegmatis | ? | - |
? | |
| additional information | allosteric enzyme: homotropic | Mycolicibacterium smegmatis CDC 46 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4* 57540, SDS-PAGE | Mycolicibacterium smegmatis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 28 | - |
assay at | Mycolicibacterium smegmatis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
up to, in 50 mM HEPES-potassium hydroxide buffer, pH 7.5, 0.1 M mercaptoethanol, 20% v/v glycerol, 30 min stable | Mycolicibacterium smegmatis |
| 70 | - |
30 min, in 50 mM HEPES-potassium hydroxide buffer, pH 7.5, 0.1 M 2-mercaptoethanol, 20% v/v glycerol, 85% loss of activity | Mycolicibacterium smegmatis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Mycolicibacterium smegmatis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | 7.1 | - |
Mycolicibacterium smegmatis |
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Release 2023.1 (January 2023)
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