Literature summary for 2.7.1.40 extracted from

Kester, H.C.M.; Uitzetter, J.H.A.A.; De Graaff, L.H.; Visser, J.
A rapid purification procedure for pyruvate kinase from the hyphal fungus Aspergillus nidulans (1988), Can. J. Microbiol., 34, 1154-1158.

Search for more literature for 2.7.1.40

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Aspergillus nidulans	5829	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
additional information	-	amino acid composition	Aspergillus nidulans
65000	-	4 * 65000, SDS-PAGE	Aspergillus nidulans

Organism

Organism	UniProt	Comment	Textmining
Aspergillus nidulans	-	wild-type strain WG 096 (yA2, paba A1) and transformant 9/402	-

Purification (Commentary)

Purification (Comment)	Organism
affinity chromatography on Mikacion brilliant yellow 6 GS-Sepharose CL-4B	Aspergillus nidulans

Source Tissue

Source Tissue	Comment	Organism	Textmining
mycelium	-	Aspergillus nidulans	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	-	Aspergillus nidulans

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ADP + phosphoenolpyruvate	-	Aspergillus nidulans	ATP + pyruvate	-	?

Subunits

Subunits	Comment	Organism
tetramer	4 * 65000, SDS-PAGE	Aspergillus nidulans

pI Value

Organism	Comment	pI Value Maximum	pI Value
Aspergillus nidulans	isoelectric focusing	4.8	4.7

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)