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Literature summary for 2.7.1.40 extracted from

  • Crow, V.L.; Pritchard, G.G.
    Pyruvate kinase from Streptococcus lactis (1982), Methods Enzymol., 90, 165-170.
No PubMed abstract available
Search for more literature for 2.7.1.40

Activating Compound

Activating Compound Comment Organism Structure
D-fructose 1,6-diphosphate activation Lactococcus lactis
D-fructose 1,6-diphosphate requirement with Mg2+ Lactococcus lactis
D-fructose 1,6-diphosphate with Mn2+ Lactococcus lactis
D-fructose 6-phosphate requirement with Mg2+, activation with Mn2+ Lactococcus lactis
D-glucose 6-phosphate requirement with Mg2+, activation with Mn2+ Lactococcus lactis
D-ribose 5-phosphate requirement with Mg2+, activation with Mn2+ Lactococcus lactis
D-tagatose 1,6-diphosphate requirement with Mg2+, activation with Mn2+ Lactococcus lactis
D-tagatose 6-phosphate requirement with Mg2+, activation with Mn2+ Lactococcus lactis
dihydroxyacetone phosphate activation Lactococcus lactis
dihydroxyacetone phosphate requirement with Mg2+ Lactococcus lactis
dihydroxyacetone phosphate with Mn2+ Lactococcus lactis
erythrose 4-phosphate requirement with Mg2+, activation with Mn2+ Lactococcus lactis
glyceraldehyde 3-phosphate activation Lactococcus lactis
glyceraldehyde 3-phosphate requirement with Mg2+ Lactococcus lactis
glyceraldehyde 3-phosphate with Mn2+ Lactococcus lactis
phosphorylated hexoses activation Lactococcus lactis
phosphorylated hexoses requirement with Mg2+ Lactococcus lactis
phosphorylated hexoses with Mn2+ Lactococcus lactis

Inhibitors

Inhibitors Comment Organism Structure
AMP in a cooperative manner with ATP Lactococcus lactis
ATP in a cooperative manner with AMP Lactococcus lactis
phosphate strong Lactococcus lactis
sulfate
-
 Lactococcus lactis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.098 0.1 GDP
-
 Lactococcus lactis

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activation Lactococcus lactis
divalent cation requirement Lactococcus lactis
K+ requirement Lactococcus lactis
K+ as good as NH4+ Lactococcus lactis
Mg2+ no activity in the absence of sugar phosphate activator Lactococcus lactis
Mn2+ best activator Lactococcus lactis
Mn2+ activation Lactococcus lactis
Mn2+ the activity of the sugar phosphate-activated enzyme is reduced to 50% if Mn2+ is omitted Lactococcus lactis
monovalent cation requirement Lactococcus lactis
NH4+ requirement Lactococcus lactis
NH4+ as good as K+ Lactococcus lactis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
235000
-
 equilibrium sedimentation Lactococcus lactis

Organism

Organism UniProt Comment Textmining
Lactococcus lactis
-
-
-
Lactococcus lactis C10
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Lactococcus lactis

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Lactococcus lactis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Lactococcus lactis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + phosphoenolpyruvate best nucleoside diphosphate substrate Lactococcus lactis ATP + pyruvate
-
 ?
ADP + phosphoenolpyruvate best nucleoside diphosphate substrate Lactococcus lactis C10 ATP + pyruvate
-
 ?
GDP + phosphoenolpyruvate less effective than ADP Lactococcus lactis GTP + pyruvate
-
 ?
GDP + phosphoenolpyruvate less effective than ADP Lactococcus lactis C10 GTP + pyruvate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Lactococcus lactis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Lactococcus lactis