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Literature summary for 2.7.1.4 extracted from
- Identification and biochemical characterization of the fructokinase gene family in Arabidopsis thaliana (2017), BMC Plant Biol., 17, 83 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Arabidopsis thaliana |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-fructose | substrate inhibition above 1 mM; substrate inhibition above 1 mM; substrate inhibition above 1 mM; substrate inhibition above 1 mM; substrate inhibition above 1 mM | Arabidopsis thaliana |  |
| EDTA | - |
Arabidopsis thaliana | |
| GTP | FRK1 activity decreases by 35% activity in the presence of 1 mM GTP; FRK2 activity decreases by about 50% activity in the presence of 1 mM GTP; FRK3 activity decreases by about 50% activity in the presence of 1 mM GTP | Arabidopsis thaliana | |
| additional information | no substrate inhibition by D-fructose (above 1 mM); no substrate inhibition by D-fructose (above 1 mM) | Arabidopsis thaliana | |
| UTP | FRK1 activity decreases by 85% activity in the presence of 1 mM UTP; FRK2 activity decreases by 75% activity in the presence of 1 mM UTP; FRK3 activity decreases by 75% activity in the presence of 1 mM UTP | Arabidopsis thaliana | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.012 | - |
D-fructose | pH 7.5, temperature not specified in the publication, FRK7 | Arabidopsis thaliana | |
| 0.052 | - |
ATP | pH 7.5, temperature not specified in the publication, FRK1 | Arabidopsis thaliana | |
| 0.052 | - |
ATP | pH 7.5, temperature not specified in the publication, FRK3 | Arabidopsis thaliana | |
| 0.085 | - |
ATP | pH 7.5, temperature not specified in the publication, FRK2 | Arabidopsis thaliana | |
| 0.095 | - |
ATP | pH 7.5, temperature not specified in the publication, FRK4 | Arabidopsis thaliana | |
| 0.16 | - |
ATP | pH 7.5, temperature not specified in the publication, FRK6 | Arabidopsis thaliana | |
| 0.18 | - |
ATP | pH 7.5, temperature not specified in the publication, FRK5 | Arabidopsis thaliana | |
| 0.23 | - |
D-fructose | pH 7.5, temperature not specified in the publication, FRK4 | Arabidopsis thaliana | |
| 0.26 | - |
D-fructose | pH 7.5, temperature not specified in the publication, FRK6 | Arabidopsis thaliana | |
| 0.28 | - |
ATP | pH 7.5, temperature not specified in the publication, FRK7 | Arabidopsis thaliana | |
| 0.32 | - |
D-fructose | pH 7.5, temperature not specified in the publication, FRK5 | Arabidopsis thaliana | |
| 0.37 | - |
D-fructose | pH 7.5, temperature not specified in the publication, FRK2 | Arabidopsis thaliana | |
| 0.47 | - |
D-fructose | pH 7.5, temperature not specified in the publication, FRK1 | Arabidopsis thaliana | |
| 0.48 | - |
D-fructose | pH 7.5, temperature not specified in the publication, FRK3 | Arabidopsis thaliana | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-fructose | Arabidopsis thaliana | to enter metabolism, fructose is phosphorylated by a fructokinase | ADP + D-fructose 6-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | O82616 | - |
- |
| Arabidopsis thaliana | Q9C524 | - |
- |
| Arabidopsis thaliana | Q9FLH8 | - |
- |
| Arabidopsis thaliana | Q9LNE3 | - |
- |
| Arabidopsis thaliana | Q9LNE4 | - |
- |
| Arabidopsis thaliana | Q9M1B9 | - |
- |
| Arabidopsis thaliana | Q9SID0 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seedling | - |
Arabidopsis thaliana | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-fructose | to enter metabolism, fructose is phosphorylated by a fructokinase | Arabidopsis thaliana | ADP + D-fructose 6-phosphate | - |
? | |
| ATP + D-fructose | no activity with ribose, xylose, fucose, glucose, tagatose or arabinose | Arabidopsis thaliana | ADP + D-fructose 6-phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| At1g06020 | - |
Arabidopsis thaliana |
| At1g66430 | - |
Arabidopsis thaliana |
| At2g31390 | - |
Arabidopsis thaliana |
| At3g59480 | - |
Arabidopsis thaliana |
| At4g10260 | - |
Arabidopsis thaliana |
| At5g51830 | - |
Arabidopsis thaliana |
| Frk1 | - |
Arabidopsis thaliana |
| Frk2 | - |
Arabidopsis thaliana |
| Frk3 | ambiguous | Arabidopsis thaliana |
| Frk4 | ambiguous | Arabidopsis thaliana |
| FRK5 | ambiguous | Arabidopsis thaliana |
| FRK6 | ambiguos | Arabidopsis thaliana |
| FRK7 | ambiguous | Arabidopsis thaliana |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.5 | - |
ATP | pH 7.5, temperature not specified in the publication, FRK7 | Arabidopsis thaliana | |
| 1.5 | - |
D-fructose | pH 7.5, temperature not specified in the publication, FRK7 | Arabidopsis thaliana | |
| 4.8 | - |
ATP | pH 7.5, temperature not specified in the publication, FRK5 | Arabidopsis thaliana | |
| 4.8 | - |
D-fructose | pH 7.5, temperature not specified in the publication, FRK5 | Arabidopsis thaliana | |
| 10.3 | - |
ATP | pH 7.5, temperature not specified in the publication, FRK2 | Arabidopsis thaliana | |
| 10.3 | - |
D-fructose | pH 7.5, temperature not specified in the publication, FRK2 | Arabidopsis thaliana | |
| 11.1 | - |
ATP | pH 7.5, temperature not specified in the publication, FRK4 | Arabidopsis thaliana | |
| 11.1 | - |
D-fructose | pH 7.5, temperature not specified in the publication, FRK4 | Arabidopsis thaliana | |
| 12.8 | - |
ATP | pH 7.5, temperature not specified in the publication, FRK6 | Arabidopsis thaliana | |
| 12.8 | - |
D-fructose | pH 7.5, temperature not specified in the publication, FRK6 | Arabidopsis thaliana | |
| 14.2 | - |
ATP | pH 7.5, temperature not specified in the publication, FRK1 | Arabidopsis thaliana | |
| 14.2 | - |
D-fructose | pH 7.5, temperature not specified in the publication, FRK1 | Arabidopsis thaliana | |
| 14.3 | - |
ATP | pH 7.5, temperature not specified in the publication, FRK3 | Arabidopsis thaliana | |
| 14.3 | - |
D-fructose | pH 7.5, temperature not specified in the publication, FRK3 | Arabidopsis thaliana | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | to enter metabolism, fructose is phosphorylated by a fructokinase | Arabidopsis thaliana |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 120 | - |
ATP | pH 7.5, temperature not specified in the publication, FRK2 | Arabidopsis thaliana | |
| 120 | - |
ATP | pH 7.5, temperature not specified in the publication, FRK4 | Arabidopsis thaliana | |
| 270 | - |
ATP | pH 7.5, temperature not specified in the publication, FRK3 | Arabidopsis thaliana | |
| 270 | - |
ATP | pH 7.5, temperature not specified in the publication, FRK5 | Arabidopsis thaliana | |
| 273 | - |
ATP | pH 7.5, temperature not specified in the publication, FRK1 | Arabidopsis thaliana | |
| 800 | - |
ATP | pH 7.5, temperature not specified in the publication, FRK6 | Arabidopsis thaliana | |
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