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Literature summary for 2.7.1.4 extracted from
- Molecular and biochemical characterization of Entamoeba histolytica fructokinase (2015), Parasitol. Res., 114, 1939-1947.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Entamoeba histolytica |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-fructose | substrate inhibition, inhibitory above 1 mM | Entamoeba histolytica |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.156 | - |
D-fructose | pH 7, 37°C | Entamoeba histolytica | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required, optimum concentration 10 mM | Entamoeba histolytica | |
| Mn2+ | 83% of the activity with Mg2+ | Entamoeba histolytica | |
| additional information | no activity with Ca2+ | Entamoeba histolytica |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33600 | - |
- |
Entamoeba histolytica |
| 35000 | - |
- |
Entamoeba histolytica |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Entamoeba histolytica | C4M2I2 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 26.3 | - |
pH 7, 37°C | Entamoeba histolytica |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C in 50% (v/v) glycerol, stable for at least 6 months | Entamoeba histolytica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-fructose | - |
Entamoeba histolytica | ADP + D-fructose 6-phosphate | - |
? | |
| ATP + D-mannose | - |
Entamoeba histolytica | ADP + D-mannose 6-phosphate | - |
? | |
| additional information | no substrates: glucose, galactose | Entamoeba histolytica | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 35000, SDS-PAGE, x * 33600, calculated | Entamoeba histolytica |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CL6EHI_054510 | - |
Entamoeba histolytica |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Entamoeba histolytica |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 14.3 | - |
D-fructose | pH 7, 37°C | Entamoeba histolytica | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Entamoeba histolytica |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Entamoeba histolytica | fructokinase messenger RNA is modestly but significantly upregulated in amoebae switched to fructose medium as well as in fructose-adapted Entamoeba histolytica | up |
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Release 2023.1 (January 2023)
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