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Literature summary for 2.7.1.4 extracted from
- The fructokinase from Rhizobium leguminosarum biovar trifolii belongs to group I fructokinase enzymes and is encoded separately from other carbohydrate metabolism enzymes (1996), Microbiology, 142, 321-330.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Rhizobium leguminosarum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.31 | - |
D-fructose | pH 8, 25°C | Rhizobium leguminosarum |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | can partially replace Mg2+ or Mn2+ in activation | Rhizobium leguminosarum | |
| Cd2+ | can partially replace Mg2+ in activation | Rhizobium leguminosarum | |
| Co2+ | can partially replace Mg2+ in activation | Rhizobium leguminosarum | |
| Mg2+ | required | Rhizobium leguminosarum | |
| Mn2+ | can partially replace Mg2+ in activation | Rhizobium leguminosarum | |
| additional information | no activation by Cu2+ | Rhizobium leguminosarum | |
| additional information | no activation by Zn2+ | Rhizobium leguminosarum | |
| additional information | no activation by Ba2+ | Rhizobium leguminosarum | |
| additional information | no activation by Fe2+ | Rhizobium leguminosarum |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36500 | - |
SDS-PAGE | Rhizobium leguminosarum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-fructose | Rhizobium leguminosarum | - |
ADP + D-fructose 6-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhizobium leguminosarum | - |
bv.trifolii | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rhizobium leguminosarum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 10.8 | - |
- |
Rhizobium leguminosarum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-fructose | - |
Rhizobium leguminosarum | ADP + D-fructose 6-phosphate | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Rhizobium leguminosarum |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Rhizobium leguminosarum | IsoGel agarose | - |
4.65 |
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Release 2023.1 (January 2023)
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