Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-fructose | severe inhibition with fructokinase-Ia and-Ib, slight with fructokinase II; substrate inhibition | Hordeum vulgare |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
D-fructose | fructokinase Ib, pH 7.6, 25°C | Hordeum vulgare | |
0.012 | - |
D-fructose | fructokinase II, pH 7.6, 25°C | Hordeum vulgare | |
0.12 | - |
D-fructose | fructokinase Ia, pH 7.6, 25°C | Hordeum vulgare |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Hordeum vulgare | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | stimulates | Hordeum vulgare |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
fructokinase-Ia, and fructokinase-1b, gel filtration | Hordeum vulgare |
73000 | - |
fructokinase II, gel filtration | Hordeum vulgare |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose | Hordeum vulgare | - |
ADP + D-fructose 6-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hordeum vulgare | - |
L. cv. Brant, barley | - |
Purification (Comment) | Organism |
---|---|
partial, 3 forms: fructokinase-Ia,-Ib,-II | Hordeum vulgare |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
51.8 | - |
fructokinase Ia and Ib | Hordeum vulgare |
60.3 | - |
fructokinase II | Hordeum vulgare |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose | - |
Hordeum vulgare | ADP + D-fructose 6-phosphate | - |
? | |
UTP + D-fructose | - |
Hordeum vulgare | UDP + D-fructose 6-phosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Hordeum vulgare |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Hordeum vulgare |