Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-fructose | above 0.4 mM at pH 8.2, no substrate inhibition at pH 6.6; substrate inhibition | Glycine max | |
MgADP- | - |
Glycine max | |
Mn2+ | can partially replace Mg2+ in activation, inhibition above 0.5 mM at 1 mM ATP | Glycine max |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.055 | - |
D-fructose | 30°C, pH 6.6 | Glycine max | |
0.077 | - |
D-fructose | 30°C, pH 8.2 | Glycine max |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Glycine max | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cs+ | slight stimulation | Glycine max | |
K+ | monovalent cation required for maximal activity, K+ most effective | Glycine max | |
Li+ | slight stimulation | Glycine max | |
Mg2+ | at 1 mM ATP maximal activity with 1.5 mM MgCl2, higher concentrations inhibit | Glycine max | |
Mg2+ | Km: 0.095 mM for MgATP2- | Glycine max | |
Mn2+ | can partially replace Mg2+ in activation | Glycine max | |
Mn2+ | inhibition above 0.5 mM at 1 mM ATP | Glycine max | |
Na+ | slight stimulation | Glycine max | |
NH4+ | can partially replace K+ in activation | Glycine max | |
Rb+ | can partially replace K+ in activation | Glycine max |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
84000 | - |
gel filtration | Glycine max |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose | Glycine max | - |
ADP + D-fructose 6-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
nodule | - |
Glycine max | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
11.2 | - |
- |
Glycine max |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose | - |
Glycine max | ADP + D-fructose 6-phosphate | - |
? | |
ATP + D-fructose | specific for fructose | Glycine max | ADP + D-fructose 6-phosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Glycine max |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
- |
Glycine max |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.4 | 9.3 | more than 90% of activity maximum at pH 7.4 and pH 9.3 | Glycine max |