BRENDA - Enzyme Database show
show all sequences of 2.7.1.39

Identification of the gene encoding homoserine kinase from Arabidopsis thaliana and characterization of the recombinant enzyme derived from the gene

Lee, M.; Leustek, T.; Arch. Biochem. Biophys. 372, 135-142 (1999)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression of a His-tagged construct in Escherichia coli
Arabidopsis thaliana
General Stability
General Stability
Organism
enzyme stored with 3 mM ATP loses 50% of its activity after two freezing cycles. In presence of 50% v/v glycerol the enzyme loses 50% of its activity after 10 cycles. In the basic storage buffer, the enzyme loses 80% of its activity after a single freeze-thaw cycle
Arabidopsis thaliana
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.32
-
ATP
-
Arabidopsis thaliana
0.4
-
L-homoserine
-
Arabidopsis thaliana
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
K+
requires 40 mM K+
Arabidopsis thaliana
Mn2+
requires 3 mM
Arabidopsis thaliana
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + L-homoserine
Arabidopsis thaliana
formation of O-phospho-L-homoserine, a branch point intermediate in the pathways for Met and Thr in plants
ADP + O-phospho-L-homoserine
-
Arabidopsis thaliana
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
Q8L7R2
-
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.09
-
L-homoserine-dependent ADP synthesis assay at pH 8.5 and 37°C
Arabidopsis thaliana
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + L-homoserine
-
641472
Arabidopsis thaliana
ADP + O-phospho-L-homoserine
-
641472
Arabidopsis thaliana
?
ATP + L-homoserine
formation of O-phospho-L-homoserine, a branch point intermediate in the pathways for Met and Thr in plants
641472
Arabidopsis thaliana
ADP + O-phospho-L-homoserine
-
641472
Arabidopsis thaliana
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
47
-
-
Arabidopsis thaliana
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
Commentary
Organism
expression of a His-tagged construct in Escherichia coli
Arabidopsis thaliana
General Stability (protein specific)
General Stability
Organism
enzyme stored with 3 mM ATP loses 50% of its activity after two freezing cycles. In presence of 50% v/v glycerol the enzyme loses 50% of its activity after 10 cycles. In the basic storage buffer, the enzyme loses 80% of its activity after a single freeze-thaw cycle
Arabidopsis thaliana
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.32
-
ATP
-
Arabidopsis thaliana
0.4
-
L-homoserine
-
Arabidopsis thaliana
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
K+
requires 40 mM K+
Arabidopsis thaliana
Mn2+
requires 3 mM
Arabidopsis thaliana
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + L-homoserine
Arabidopsis thaliana
formation of O-phospho-L-homoserine, a branch point intermediate in the pathways for Met and Thr in plants
ADP + O-phospho-L-homoserine
-
Arabidopsis thaliana
?
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.09
-
L-homoserine-dependent ADP synthesis assay at pH 8.5 and 37°C
Arabidopsis thaliana
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + L-homoserine
-
641472
Arabidopsis thaliana
ADP + O-phospho-L-homoserine
-
641472
Arabidopsis thaliana
?
ATP + L-homoserine
formation of O-phospho-L-homoserine, a branch point intermediate in the pathways for Met and Thr in plants
641472
Arabidopsis thaliana
ADP + O-phospho-L-homoserine
-
641472
Arabidopsis thaliana
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
47
-
-
Arabidopsis thaliana
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Arabidopsis thaliana
Other publictions for EC 2.7.1.39
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737948
Brewer
Mutations in the Arabidopsis h ...
Arabidopsis thaliana
BMC Plant Biol.
14
317
2014
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1
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722043
De Pascale
Identification and characteriz ...
Schizosaccharomyces pombe
ChemBioChem
12
1179-1182
2011
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1
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6
2
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722151
Kingsbury
Homoserine toxicity in Sacchar ...
Candida albicans, Saccharomyces cerevisiae, Saccharomyces cerevisiae YJM145
Eukaryot. Cell
9
717-728
2010
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2
2
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722152
Kingsbury
Fungal homoserine kinase (thr1 ...
Candida albicans, Saccharomyces cerevisiae
Eukaryot. Cell
9
729-737
2010
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2
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704525
Stockbridge
The intrinsic reactivity of AT ...
Methanocaldococcus jannaschii
J. Biol. Chem.
284
22747-22757
2009
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706213
van Damme
Downy mildew resistance in Ara ...
Arabidopsis thaliana
Plant Cell
21
2179-2189
2009
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1
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1
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5
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1
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690392
Rinder
Regulation of aspartate-derive ...
Escherichia coli
Amino Acids
34
213-222
2008
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1
1
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1
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3
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694038
Kingsbury
Threonine biosynthetic genes a ...
Cryptococcus neoformans
Microbiology
154
2767-2775
2008
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1
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663091
Lee
Methionine and threonine synth ...
Arabidopsis thaliana
Plant J.
41
685-696
2005
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2
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673979
Paik
Identification of virulence de ...
Streptococcus sanguinis, Streptococcus sanguinis SK36
Infect. Immun.
73
6064-6074
2005
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9
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661325
Bareich
Small molecule functional disc ...
Schizosaccharomyces pombe
Bioorg. Med. Chem.
12
807-815
2004
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662779
Fernandez
Role of homoserine and threoni ...
Streptomyces sp., Streptomyces sp. NRRL 5331
Microbiology
150
1467-1474
2004
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641475
Krishna
Structural basis for the catal ...
Methanocaldococcus jannaschii
Biochemistry
40
10810-10818
2001
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641474
Zhou
Structure and mechanism of hom ...
Methanocaldococcus jannaschii
Structure Fold. Des.
8
1247-1257
2000
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641472
Lee
Identification of the gene enc ...
Arabidopsis thaliana
Arch. Biochem. Biophys.
372
135-142
1999
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641476
Patte
ThrH, a homoserine kinase isoz ...
Pseudomonas aeruginosa
Microbiology
145
845-853
1999
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641471
Huo
Substrate specificity and iden ...
Escherichia coli
Biochemistry
35
16180-16185
1996
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26
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641473
Huo
Functional group characterizat ...
Escherichia coli
Arch. Biochem. Biophys.
330
373-379
1996
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641467
Riesmeier
-
Purification to homogeneity an ...
Triticum aestivum
Phytochemistry
32
581-584
1993
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641468
Ramos
Inhibition by different amino ...
Saccharomyces cerevisiae
FEBS Lett.
278
123-126
1991
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2
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641469
Mannhaupt
Yeast homoserine kinase. Chara ...
Saccharomyces cerevisiae
Eur. J. Biochem.
191
115-122
1990
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641464
Finkelnburg
-
Homoserine kinase from phototr ...
Rhodospirillum rubrum, Rhodospirillum rubrum S1
FEMS Microbiol. Lett.
48(1-2)
93-96
1987
1
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1
-
2
-
-
1
2
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-
1
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-
-
4
-
-
-
-
-
-
-
-
-
-
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-
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-
641463
Shames
Homoserine kinase of Escherich ...
Escherichia coli
Arch. Biochem. Biophys.
235
359-370
1984
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-
-
-
-
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4
3
-
-
-
-
-
2
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-
1
1
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-
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3
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1
-
-
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3
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4
3
3
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-
1
-
-
-
-
3
-
-
-
1
-
-
-
-
-
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-
641462
Baum
-
Feedback inhibition of homoser ...
Raphanus sativus
Phytochemistry
22
2409-2412
1983
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-
-
-
-
-
3
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-
2
-
2
-
1
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1
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3
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1
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3
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2
-
2
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1
-
-
3
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-
-
-
-
1
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-
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-
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-
641461
Thoen
-
Biosynthesis of threonine from ...
Pisum sativum
Plant Sci. Lett.
13
103-112
1978
1
-
-
-
-
-
13
2
-
5
1
1
-
1
-
-
1
-
-
2
1
1
2
-
-
-
1
-
1
1
-
-
3
-
-
1
-
-
-
-
-
-
-
13
3
2
-
5
1
1
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-
1
-
2
1
1
2
-
-
-
1
-
1
1
-
-
-
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-
-
-
-
641460
Aarnes
-
Homoserine kinase from barley ...
Hordeum vulgare
Plant Sci. Lett.
7
187-194
1976
1
-
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1
2
-
5
-
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1
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1
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-
1
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-
1
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1
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1
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1
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2
-
5
-
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1
-
1
-
-
1
-
-
-
1
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641470
Burr
Homoserine kinase from Escheri ...
Escherichia coli
Eur. J. Biochem.
62
519-526
1976
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-
-
-
-
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9
2
-
1
2
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-
2
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-
1
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-
1
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1
1
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-
-
-
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9
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9
9
2
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1
2
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1
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1
-
1
1
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641465
Theze
Homoserine kinase from Escheri ...
Escherichia coli
J. Bacteriol.
118
577-581
1974
1
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3
2
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2
1
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2
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1
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1
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1
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1
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1
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3
1
2
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2
1
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-
1
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1
-
1
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-
641466
Miyajima
Regulation of aspartate family ...
Brevibacterium flavum
J. Biochem.
71
219-226
1972
1
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2
2
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3
1
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2
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1
1
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-
1
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1
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-
1
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1
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2
-
2
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3
1
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-
1
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1
-
1
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1
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