Literature summary for 2.7.1.39 extracted from

Lee, M.; Leustek, T.
Identification of the gene encoding homoserine kinase from Arabidopsis thaliana and characterization of the recombinant enzyme derived from the gene (1999), Arch. Biochem. Biophys., 372, 135-142.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression of a His-tagged construct in Escherichia coli	Arabidopsis thaliana

General Stability

General Stability	Organism
enzyme stored with 3 mM ATP loses 50% of its activity after two freezing cycles. In presence of 50% v/v glycerol the enzyme loses 50% of its activity after 10 cycles. In the basic storage buffer, the enzyme loses 80% of its activity after a single freeze-thaw cycle	Arabidopsis thaliana

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.32	-	ATP	-	Arabidopsis thaliana
0.4	-	L-homoserine	-	Arabidopsis thaliana

Metals/Ions

Metals/Ions	Comment	Organism	Structure
K+	requires 40 mM K+	Arabidopsis thaliana
Mn2+	requires 3 mM	Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + L-homoserine	Arabidopsis thaliana	formation of O-phospho-L-homoserine, a branch point intermediate in the pathways for Met and Thr in plants	ADP + O-phospho-L-homoserine	-	?

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	Q8L7R2	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
3.09	-	L-homoserine-dependent ADP synthesis assay at pH 8.5 and 37°C	Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + L-homoserine	-	Arabidopsis thaliana	ADP + O-phospho-L-homoserine	-	?
ATP + L-homoserine	formation of O-phospho-L-homoserine, a branch point intermediate in the pathways for Met and Thr in plants	Arabidopsis thaliana	ADP + O-phospho-L-homoserine	-	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
47	-	-	Arabidopsis thaliana

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	-	Arabidopsis thaliana

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Release 2023.1 (January 2023)