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Literature summary for 2.7.1.39 extracted from

  • Theze, J.; Kleidman, L.; Saint Girons, I.
    Homoserine kinase from Escherichia coli K-12: properties, inhibition by L-threonine, and regulation of biosynthesis (1974), J. Bacteriol., 118, 577-581.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
NH4+ 30% of the activity obtained with 0.5 M K+ Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
alpha-amino-beta-hydroxy valeric acid
-
Escherichia coli
L-2-aminobutyric acid 40 mM, 50% inhibition Escherichia coli
L-Thr
-
Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
ATP pH 7.8, 27°C Escherichia coli
0.3
-
L-homoserine pH 7.8, 27°C Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
K+ activates Escherichia coli
Mg2+ absolute requirement, optimal activity between 10 mM and 20 mM Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
K-12
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-homoserine
-
Escherichia coli ADP + O-phospho-L-homoserine
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
57
-
half-life is 10 min in absence of L-Thr ot L-homoserine Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1
-
L-Thr pH 7.8, 27°C Escherichia coli