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Literature summary for 2.7.1.36 extracted from
- Biochemical characterization of recombinant mevalonate kinase from Bacopa monniera (2015), Int. J. Biol. Macromol., 72, 776-783.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Bacopa monnieri |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1474 | - |
ATP | at pH 7.0 and 30°C | Bacopa monnieri |  |
| 0.3319 | - |
(R)-mevalonate | at pH 7.0 and 30°C | Bacopa monnieri | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required for full activity, not replaceable by other divalent ions | Bacopa monnieri | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 46000 | - |
1 * 46000, SDS-PAGE | Bacopa monnieri |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + (R)-mevalonate | Bacopa monnieri | - |
ADP + (R)-5-phosphomevalonate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacopa monnieri | M1F2U2 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography and gel filtration | Bacopa monnieri |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + (R)-mevalonate | - |
Bacopa monnieri | ADP + (R)-5-phosphomevalonate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 46000, SDS-PAGE | Bacopa monnieri |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ATP: mevalonate 5-phosphotransferase | - |
Bacopa monnieri |
| mevalonate phosphokinase | - |
Bacopa monnieri |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Bacopa monnieri |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 35 | more than 50% activity between 25°C and 35°C | Bacopa monnieri |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 30 | the enzyme is stable at 20-25°C for 80 min and at optimum temperature 30°C for 40 min | Bacopa monnieri |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 143.8 | - |
(R)-mevalonate | at pH 7.0 and 30°C | Bacopa monnieri | |
| 150.9 | - |
ATP | at pH 7.0 and 30°C | Bacopa monnieri | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Bacopa monnieri |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 9.5 | more than 50% activity between pH 6.5 and 9.5 | Bacopa monnieri |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | the enzyme is most stable at pH 7.0-8.0 at 25°C with half-life of 27-49 h | Bacopa monnieri |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 433.3 | - |
(R)-mevalonate | at pH 7.0 and 30°C | Bacopa monnieri | |
| 1023 | - |
ATP | at pH 7.0 and 30°C | Bacopa monnieri | |
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Release 2023.1 (January 2023)
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